Click here to switch to the map view.

The map label for this gene is panB [H]

Identifier: 170025687

GI number: 170025687

Start: 3814327

End: 3815124

Strand: Direct

Name: panB [H]

Synonym: YPK_3472

Alternate gene names: 170025687

Gene position: 3814327-3815124 (Clockwise)

Preceding gene: 170025686

Following gene: 170025688

Centisome position: 81.34

GC content: 52.13

Gene sequence:

>798_bases
ATGAAAACCACCACCATGAGCCAATTACGCCAATGGAAACAAGAGAAACGTAAATTCGCAACGCTGACGGCCTATGATGC
CAGTTTTGCCCAGTTATTTGCTGAACAGGGTATTCAGGTGCTATTGGTGGGGGATTCGCTCGGTATGACGTTACAAGGGT
TCGATTCCACGCTGCCAGTGACCGTTGCCGATGTGGCCTACCATACCCGTGCAGTGCGCCGTGGTGCGCCTCATTGCCTG
TTATTGGCCGATATGCCATTCATGAGTTATGCCACGCCAGAACTGGCCTTTACCCACGCGGCAGAGCTAATGCGGGCCGG
AGCCAATATGGTCAAACTGGAAGGTGGGAGCTGGCTGTGCGATACCATTCGCATGTTGGCAGAGCGCGCGGTGCCGGTTT
GTGGGCATCTGGGGCTCACGCCACAATCGGTGAATATTTTCGGCGGCTATAAAGTACAGGGTCGTGAAGAAGTGGCGGCC
AATCAACTGTTGCAAGATGCTATTGCCCTGGAACAGGCAGGGGCTCAACTGTTGGTACTGGAATGCGTGCCGGTAGAACT
GGCACAGCGGGTGACCGAAGAGTTAACCATACCGGTTATTGGTATTGGTGCGGGGAATGTCACTGACGGTCAGATTTTGG
TTATGCATGATGCACTCGGTATTACCGGGGGTCATACGCCAAAATTCAGTAAAAACTTTTTAGCACACAGTGCGGGCGAT
ATTCGTGCCGCCATTAAGCTCTATATTGAAGAAGTTGAGGGGGGGATTTATCCTGCTGAAGAGCATACATTCCAGTAA

Upstream 100 bases:

>100_bases
ATGTCTTGCAGATAATCTTATATTCCATCCCGTAATGGGCAGGTCTTGGTTTATCCGTTTGTTGATAAAGTTGTTCACTT
TAATTTCAGGAGACAGAGTG

Downstream 100 bases:

>100_bases
CTGAGCCACTAATATTTCTTCTAAAATAGGGAGTTTTCAAAATAGGATCCTCCTAAAATAGATCGTTCCTAAAACAGGAG
AAAGGGAATGCTGATTATTG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MKTTTMSQLRQWKQEKRKFATLTAYDASFAQLFAEQGIQVLLVGDSLGMTLQGFDSTLPVTVADVAYHTRAVRRGAPHCL
LLADMPFMSYATPELAFTHAAELMRAGANMVKLEGGSWLCDTIRMLAERAVPVCGHLGLTPQSVNIFGGYKVQGREEVAA
NQLLQDAIALEQAGAQLLVLECVPVELAQRVTEELTIPVIGIGAGNVTDGQILVMHDALGITGGHTPKFSKNFLAHSAGD
IRAAIKLYIEEVEGGIYPAEEHTFQ

Sequences:

>Translated_265_residues
MKTTTMSQLRQWKQEKRKFATLTAYDASFAQLFAEQGIQVLLVGDSLGMTLQGFDSTLPVTVADVAYHTRAVRRGAPHCL
LLADMPFMSYATPELAFTHAAELMRAGANMVKLEGGSWLCDTIRMLAERAVPVCGHLGLTPQSVNIFGGYKVQGREEVAA
NQLLQDAIALEQAGAQLLVLECVPVELAQRVTEELTIPVIGIGAGNVTDGQILVMHDALGITGGHTPKFSKNFLAHSAGD
IRAAIKLYIEEVEGGIYPAEEHTFQ
>Mature_265_residues
MKTTTMSQLRQWKQEKRKFATLTAYDASFAQLFAEQGIQVLLVGDSLGMTLQGFDSTLPVTVADVAYHTRAVRRGAPHCL
LLADMPFMSYATPELAFTHAAELMRAGANMVKLEGGSWLCDTIRMLAERAVPVCGHLGLTPQSVNIFGGYKVQGREEVAA
NQLLQDAIALEQAGAQLLVLECVPVELAQRVTEELTIPVIGIGAGNVTDGQILVMHDALGITGGHTPKFSKNFLAHSAGD
IRAAIKLYIEEVEGGIYPAEEHTFQ

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=265, Percent_Identity=76.2264150943396, Blast_Score=430, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6319653, Length=276, Percent_Identity=36.9565217391304, Blast_Score=168, Evalue=1e-42,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 28667; Mature: 28667

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTTTMSQLRQWKQEKRKFATLTAYDASFAQLFAEQGIQVLLVGDSLGMTLQGFDSTLPV
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCE
TVADVAYHTRAVRRGAPHCLLLADMPFMSYATPELAFTHAAELMRAGANMVKLEGGSWLC
EHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
DTIRMLAERAVPVCGHLGLTPQSVNIFGGYKVQGREEVAANQLLQDAIALEQAGAQLLVL
HHHHHHHHHCCCHHCCCCCCCCCEEEEECEEECCHHHHHHHHHHHHHHHHHHCCCEEEEE
ECVPVELAQRVTEELTIPVIGIGAGNVTDGQILVMHDALGITGGHTPKFSKNFLAHSAGD
EECCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCH
IRAAIKLYIEEVEGGIYPAEEHTFQ
HHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKTTTMSQLRQWKQEKRKFATLTAYDASFAQLFAEQGIQVLLVGDSLGMTLQGFDSTLPV
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCCCEEEEEECCCCCEEECCCCCCCE
TVADVAYHTRAVRRGAPHCLLLADMPFMSYATPELAFTHAAELMRAGANMVKLEGGSWLC
EHHHHHHHHHHHHCCCCCEEEEECCCCHHHCCCHHHHHHHHHHHHCCCCEEEECCCCCHH
DTIRMLAERAVPVCGHLGLTPQSVNIFGGYKVQGREEVAANQLLQDAIALEQAGAQLLVL
HHHHHHHHHCCCHHCCCCCCCCCEEEEECEEECCHHHHHHHHHHHHHHHHHHCCCEEEEE
ECVPVELAQRVTEELTIPVIGIGAGNVTDGQILVMHDALGITGGHTPKFSKNFLAHSAGD
EECCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHCCCCH
IRAAIKLYIEEVEGGIYPAEEHTFQ
HHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA