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The map label for this gene is gshA [H]

Identifier: 170025584

GI number: 170025584

Start: 3694675

End: 3696273

Strand: Reverse

Name: gshA [H]

Synonym: YPK_3369

Alternate gene names: 170025584

Gene position: 3696273-3694675 (Counterclockwise)

Preceding gene: 170025585

Following gene: 170025583

Centisome position: 78.82

GC content: 48.09

Gene sequence:

>1599_bases
ATGCTGATGAGTTATGGTTTTCAAGAGCGGGAGGTCGATTTGATCCCGGATGTATCACACGCGTTGACTTGGTTGGAAGC
GCATCCTAAAGCCCTAAAAGGGATCCGTCGCGGTATCGAGCGGGAAACATTGAGAGTGACTGCCGATGGCCATTTAGCCT
CGACGGGCCATCCAGAATCACTGGGTGCCGCTTTGACACATCAATGGATCACTACCGATTTCGCTGAGGCATTACTTGAG
TTTATTACTCCTGTCGATGGTGACATTGATCATCTGTTGACTTTTTTACGTGATATTCATCGTTATACTGCACGTAAGTT
GGGTGATGAACGCATGTGGCCGCTGAGCATGCCTTGTTTTATTGAGGCTGAACAGGATATTGAGCTGGCTAAATATGGTT
CGTCTAATATTGGCCGCTTTAAAACACTTTACCGTGAAGGGTTAAAAAACCGTTACGGTGCGCTGATGCAAACGATTTCT
GGTGTGCACTATAATTTTTCTTTGCCACTGGAGTTTTGGCAGGCATGGGCAGGCGTCACCGATGAGCAAAGTGGGAAGGA
GGAGATTTCCGCAGGCTATTTCCGTCTGATCCGGAACTATTACCGCTTTGGCTGGGTTATTCCTTATCTGTTTGGCGCAT
CACCGGCCATTTGCTCATCGTTCCTGCAGGGGCGCGAGACGGCGTTACCGTTTGAGCGTAATGGTAAGGGAATGTGTTAT
TTACCCTATGCTACGTCATTGCGGTTGAGTGATTTAGGCTATACCAATAAATCGCAAAGTAATTTAGGTATCACATTTAA
TGACCTGCATACCTACGTTGACGCTCTGAAACGTGCGATTCAAACACCATCAGAGGAGTACGTGGCTTTAGGTCTGAAAG
ACGGTGATCGCCATCTGCAACTGAATACTAATGTGTTGCAGATAGAGAATGAGTTGTATGCCCCTATTCGGCCTAAACGG
GTTACCCGCGCGGGAGAATCACCGTCAGACGCTTTGCTTCGTGGTGGCATTGAATATATCGAAGTGCGTTCGCTGGACAT
AAATCCATTCTCACCTATTGGTGTTGATGCGGTGCAAGCGCGTTTCCTCGATTTATTCCTGATTTGGTGTGTGCTGGCTG
ATGCACCAGAGATGAGCAGTGATGAACTGCTGTGTACGCGTAAGAATTGGGATCGGGTCATTTTAGAAGGGCGTAAACCG
GGGCAGACCATTGGGATAGGGTGTAGTGATTCCCGTCAACCGCTGGAAACGGTTGGTAAGGCACTGTTTGCTGATTTACG
TCGGGTTGCAGAGGTTCTGGATGGGAGCGAGAGTGCATCGTATCAGCAGGTTTGCGATGAACTTGTCGCCTCGTTTGATG
ATCCTGAACTGACGTTCTCTGCCCGTATTCTAAAGGTAATGCAAGAGAAGGGGATCGGTGGAGTTGGGCTTGAGTTGGCT
GAGCATTATCGAGAAATGCTGCAAAACGAACCACTGGAACTGCTGACGGAAGAACAGTTATCTGCAGAGCGCGATGCTTC
ACGGCAACGGCAATATGAGCTAGAACTCAAAGATAAGCTAAGCTTTGAAGAGTATTTAGCGCTTCATGGTGGCCAATAA

Upstream 100 bases:

>100_bases
TTGCGTTACATCGTGTTAACGATGATTACGTTGCAAGGGATCGCTTGGTGGCAATAATAGATGTGACAAACTTACGTAGT
CACATCTATGCAGTTTCAGT

Downstream 100 bases:

>100_bases
AAAAGAAAAGGCCACATCACTGTGGCCAAATTAACATCTCTAATAGAAGGGATGATGATAACAAATGCGCGTCTTTCACA
TATTAAGACGCGCAGGATGA

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLMSYGFQEREVDLIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLE
FITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTIS
GVHYNFSLPLEFWQAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCY
LPYATSLRLSDLGYTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKR
VTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKP
GQTIGIGCSDSRQPLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELA
EHYREMLQNEPLELLTEEQLSAERDASRQRQYELELKDKLSFEEYLALHGGQ

Sequences:

>Translated_532_residues
MLMSYGFQEREVDLIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLE
FITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTIS
GVHYNFSLPLEFWQAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCY
LPYATSLRLSDLGYTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKR
VTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKP
GQTIGIGCSDSRQPLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELA
EHYREMLQNEPLELLTEEQLSAERDASRQRQYELELKDKLSFEEYLALHGGQ
>Mature_532_residues
MLMSYGFQEREVDLIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPESLGAALTHQWITTDFAEALLE
FITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCFIEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTIS
GVHYNFSLPLEFWQAWAGVTDEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCY
LPYATSLRLSDLGYTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQLNTNVLQIENELYAPIRPKR
VTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQARFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKP
GQTIGIGCSDSRQPLETVGKALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELA
EHYREMLQNEPLELLTEEQLSAERDASRQRQYELELKDKLSFEEYLALHGGQ

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=73.7451737451737, Blast_Score=803, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60093; Mature: 60093

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSYGFQEREVDLIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPES
CCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
LGAALTHQWITTDFAEALLEFITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVT
EECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCC
DEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEE
LPYATSLRLSDLGYTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQ
ECCCCCEEEHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEE
LNTNVLQIENELYAPIRPKRVTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQA
ECCCEEEECCCCCCCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
RFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQPLETVGK
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHH
ALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
EHYREMLQNEPLELLTEEQLSAERDASRQRQYELELKDKLSFEEYLALHGGQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MLMSYGFQEREVDLIPDVSHALTWLEAHPKALKGIRRGIERETLRVTADGHLASTGHPES
CCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHH
LGAALTHQWITTDFAEALLEFITPVDGDIDHLLTFLRDIHRYTARKLGDERMWPLSMPCF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEE
IEAEQDIELAKYGSSNIGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLEFWQAWAGVT
EECCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCC
DEQSGKEEISAGYFRLIRNYYRFGWVIPYLFGASPAICSSFLQGRETALPFERNGKGMCY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEE
LPYATSLRLSDLGYTNKSQSNLGITFNDLHTYVDALKRAIQTPSEEYVALGLKDGDRHLQ
ECCCCCEEEHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEE
LNTNVLQIENELYAPIRPKRVTRAGESPSDALLRGGIEYIEVRSLDINPFSPIGVDAVQA
ECCCEEEECCCCCCCCCCHHHHCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHH
RFLDLFLIWCVLADAPEMSSDELLCTRKNWDRVILEGRKPGQTIGIGCSDSRQPLETVGK
HHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHH
ALFADLRRVAEVLDGSESASYQQVCDELVASFDDPELTFSARILKVMQEKGIGGVGLELA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHH
EHYREMLQNEPLELLTEEQLSAERDASRQRQYELELKDKLSFEEYLALHGGQ
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA