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The map label for this gene is nqrE [H]

Identifier: 170025518

GI number: 170025518

Start: 3614621

End: 3615217

Strand: Reverse

Name: nqrE [H]

Synonym: YPK_3301

Alternate gene names: 170025518

Gene position: 3615217-3614621 (Counterclockwise)

Preceding gene: 170025519

Following gene: 170025517

Centisome position: 77.09

GC content: 48.07

Gene sequence:

>597_bases
ATGGAACATTATATCAGTCTGTTGGTCCGCGCAGTGTTTGTGGAGAATATGGCGTTAGCGTTCTTTCTGGGAATGTGTAC
TTTCCTGGCCGTCTCTAAAAAAGTGTCAACGGCCTTTGGCTTAGGCATTGCGGTAACGGTTGTTCTGGGCATCTCCGTGC
CAGCCAATAATTTGGTTTATAACCTGGTTCTGCGAGATGGCGCATTGGTTGAAGGGGTTGACCTTAGCTTCCTGAATTTC
ATTACCTTCATTGGGGTCATTGCCGCCATTGTTCAGGTGTTGGAGATGATCCTCGATCGTTACTTCCCTGCGTTATATAA
CGCGCTGGGTATTTTTCTGCCGCTAATCACCGTGAACTGCGCCATCTTTGGTGGCGTGTCGTTTATGGCACAGCGTGATT
ACAACTTCCCTGAATCCATCGTTTATGGCTTTGGTTCTGGTATGGGGTGGATGCTGGCGATTGTTGCATTAGCGGGGATC
CGCGAAAAAATGAAATATGCCAATGTGCCTGCGGGCTTACAGGGGCTAGGGATCACCTTTATCTCAACCGGATTGATGGC
ATTAGGTTTTATGTCTTTCGCCGGTGTGAACCTATAA

Upstream 100 bases:

>100_bases
ATTATTCCTGCTGGCACCTAGTGCATTCTTTATTATTGGTCTGCTGATTTGGGGCCTACGGACCTTGAAACCTGCGCAGA
TTGAGAAGGAGTAATTGACG

Downstream 100 bases:

>100_bases
AGGCAGGAATAATTCATGGAAATAATTCTGGGCGTAGTTATGTTCACCCTCATCGTGCTGGCGCTGACGGTGATGATTTT
ATTCGCTAAATCAAAATTGG

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: Na(+)-NQR subunit E; Na(+)-translocating NQR subunit E; NQR complex subunit E; NQR-1 subunit E [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MEHYISLLVRAVFVENMALAFFLGMCTFLAVSKKVSTAFGLGIAVTVVLGISVPANNLVYNLVLRDGALVEGVDLSFLNF
ITFIGVIAAIVQVLEMILDRYFPALYNALGIFLPLITVNCAIFGGVSFMAQRDYNFPESIVYGFGSGMGWMLAIVALAGI
REKMKYANVPAGLQGLGITFISTGLMALGFMSFAGVNL

Sequences:

>Translated_198_residues
MEHYISLLVRAVFVENMALAFFLGMCTFLAVSKKVSTAFGLGIAVTVVLGISVPANNLVYNLVLRDGALVEGVDLSFLNF
ITFIGVIAAIVQVLEMILDRYFPALYNALGIFLPLITVNCAIFGGVSFMAQRDYNFPESIVYGFGSGMGWMLAIVALAGI
REKMKYANVPAGLQGLGITFISTGLMALGFMSFAGVNL
>Mature_198_residues
MEHYISLLVRAVFVENMALAFFLGMCTFLAVSKKVSTAFGLGIAVTVVLGISVPANNLVYNLVLRDGALVEGVDLSFLNF
ITFIGVIAAIVQVLEMILDRYFPALYNALGIFLPLITVNCAIFGGVSFMAQRDYNFPESIVYGFGSGMGWMLAIVALAGI
REKMKYANVPAGLQGLGITFISTGLMALGFMSFAGVNL

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. NqrA to nqrE are probably involved in the second step, the conversion of ubisemiqui

COG id: COG2209

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=196, Percent_Identity=41.3265306122449, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR010967 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 21247; Mature: 21247

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHYISLLVRAVFVENMALAFFLGMCTFLAVSKKVSTAFGLGIAVTVVLGISVPANNLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
NLVLRDGALVEGVDLSFLNFITFIGVIAAIVQVLEMILDRYFPALYNALGIFLPLITVNC
HHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIFGGVSFMAQRDYNFPESIVYGFGSGMGWMLAIVALAGIREKMKYANVPAGLQGLGITF
HHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ISTGLMALGFMSFAGVNL
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEHYISLLVRAVFVENMALAFFLGMCTFLAVSKKVSTAFGLGIAVTVVLGISVPANNLVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
NLVLRDGALVEGVDLSFLNFITFIGVIAAIVQVLEMILDRYFPALYNALGIFLPLITVNC
HHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIFGGVSFMAQRDYNFPESIVYGFGSGMGWMLAIVALAGIREKMKYANVPAGLQGLGITF
HHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
ISTGLMALGFMSFAGVNL
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]