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The map label for this gene is csgA [H]

Identifier: 170025487

GI number: 170025487

Start: 3574754

End: 3575434

Strand: Reverse

Name: csgA [H]

Synonym: YPK_3270

Alternate gene names: 170025487

Gene position: 3575434-3574754 (Counterclockwise)

Preceding gene: 170025488

Following gene: 170025485

Centisome position: 76.24

GC content: 50.22

Gene sequence:

>681_bases
ATGGGCACAGCGAAAAGAACAGCATTGGTTATCGGTGCTTCACGGGGGTTAGGGCTAGGGTTGGTTGATGAGCTTAGTCG
CCGCGGATGGTCTGTCACCGCGACCACACGTGGCGTGGCTCAAGATACGTCAGCACATGCCGCCCATTGGTTAAAATTAG
ATATTAATCAGCCAGAAAGCATTAAGCAGTTTTTACCACAAGTACAGGGGCAGCGATTCGAGCTGATATTTGTTAACGCC
GGAATCTCTGGGCCAGCACACCAATCTGCGATCGATGCCACACCCGATGAAATCCAGGAACTGTTCCAGACTAACGCCAT
CTCGCCTATCCGAATTGCTCAACATTTGCTGGCACAACGCAATCCAACCCAGAGCGTATTGGCGTTTATGTCTTCCCAGT
TAGGCAGTATTGAACGCAATGTTTCTGGGCATAAACCGTTGTATTCAGCCAGTAAAGCCGCATTGAATATGATGACGCGT
AATCTGGTGGCTGAAGTTGCAGATCCGACCTTGACGGTATTATCGATTCATCCCGGTTGGGTGAAAACGGATATGGGCGG
TGATGCGGCACCGTTGACGATTACTACCAGTGTGAAGGGCGTGGTCGATCAAATCGAACAGGCTTCAGGGAAAGGGGGCC
ACGGATTTATTGACTATCAGGGGCATTCATTACCGTGGTAA

Upstream 100 bases:

>100_bases
CAGTGATAACTGAGCTGTTTTTTCGCTCCAGTAAGATAATATTTCATAGGGATAAGAGAATATTTATAGCGCGAATAACA
CTCACTGAAAGGAGCATGAT

Downstream 100 bases:

>100_bases
TGCTCTTTGTTCACCATTAATACGCTCCACTCTAATGAATATCCCCCATCAATAGTTCGATAGGGGATAATATTTTCAGA
TACTTCGCCGCCGATGGCTT

Product: short chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: C signal [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MGTAKRTALVIGASRGLGLGLVDELSRRGWSVTATTRGVAQDTSAHAAHWLKLDINQPESIKQFLPQVQGQRFELIFVNA
GISGPAHQSAIDATPDEIQELFQTNAISPIRIAQHLLAQRNPTQSVLAFMSSQLGSIERNVSGHKPLYSASKAALNMMTR
NLVAEVADPTLTVLSIHPGWVKTDMGGDAAPLTITTSVKGVVDQIEQASGKGGHGFIDYQGHSLPW

Sequences:

>Translated_226_residues
MGTAKRTALVIGASRGLGLGLVDELSRRGWSVTATTRGVAQDTSAHAAHWLKLDINQPESIKQFLPQVQGQRFELIFVNA
GISGPAHQSAIDATPDEIQELFQTNAISPIRIAQHLLAQRNPTQSVLAFMSSQLGSIERNVSGHKPLYSASKAALNMMTR
NLVAEVADPTLTVLSIHPGWVKTDMGGDAAPLTITTSVKGVVDQIEQASGKGGHGFIDYQGHSLPW
>Mature_225_residues
GTAKRTALVIGASRGLGLGLVDELSRRGWSVTATTRGVAQDTSAHAAHWLKLDINQPESIKQFLPQVQGQRFELIFVNAG
ISGPAHQSAIDATPDEIQELFQTNAISPIRIAQHLLAQRNPTQSVLAFMSSQLGSIERNVSGHKPLYSASKAALNMMTRN
LVAEVADPTLTVLSIHPGWVKTDMGGDAAPLTITTSVKGVVDQIEQASGKGGHGFIDYQGHSLPW

Specific function: Cell-cell signaling protein required for fruiting body morphogenesis. Necessary for cellular aggregation, for spore differentiation, and for gene expression that is initiated after 6 hour of starvation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To yeast YKL071W [H]

Homologues:

Organism=Escherichia coli, GI1787335, Length=188, Percent_Identity=28.7234042553192, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17558994, Length=231, Percent_Identity=30.7359307359307, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17558990, Length=250, Percent_Identity=29.2, Blast_Score=83, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17559962, Length=228, Percent_Identity=31.140350877193, Blast_Score=81, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193207563, Length=228, Percent_Identity=27.6315789473684, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17532177, Length=247, Percent_Identity=26.7206477732794, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17541382, Length=257, Percent_Identity=25.6809338521401, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17558992, Length=228, Percent_Identity=28.5087719298246, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558996, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17559964, Length=227, Percent_Identity=29.9559471365639, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17542178, Length=244, Percent_Identity=25.4098360655738, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322779, Length=204, Percent_Identity=31.8627450980392, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322067, Length=202, Percent_Identity=29.2079207920792, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640547, Length=244, Percent_Identity=32.7868852459016, Blast_Score=117, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 24151; Mature: 24020

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTAKRTALVIGASRGLGLGLVDELSRRGWSVTATTRGVAQDTSAHAAHWLKLDINQPES
CCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHH
IKQFLPQVQGQRFELIFVNAGISGPAHQSAIDATPDEIQELFQTNAISPIRIAQHLLAQR
HHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
NPTQSVLAFMSSQLGSIERNVSGHKPLYSASKAALNMMTRNLVAEVADPTLTVLSIHPGW
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCE
VKTDMGGDAAPLTITTSVKGVVDQIEQASGKGGHGFIDYQGHSLPW
EEECCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
GTAKRTALVIGASRGLGLGLVDELSRRGWSVTATTRGVAQDTSAHAAHWLKLDINQPES
CCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEECCCCHH
IKQFLPQVQGQRFELIFVNAGISGPAHQSAIDATPDEIQELFQTNAISPIRIAQHLLAQR
HHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCC
NPTQSVLAFMSSQLGSIERNVSGHKPLYSASKAALNMMTRNLVAEVADPTLTVLSIHPGW
CCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCE
VKTDMGGDAAPLTITTSVKGVVDQIEQASGKGGHGFIDYQGHSLPW
EEECCCCCCCCEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2152896; 2107980; 2118510; 1372277 [H]