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The map label for this gene is nusB [H]

Identifier: 170025473

GI number: 170025473

Start: 3560712

End: 3561128

Strand: Reverse

Name: nusB [H]

Synonym: YPK_3256

Alternate gene names: 170025473

Gene position: 3561128-3560712 (Counterclockwise)

Preceding gene: 170025474

Following gene: 170025472

Centisome position: 75.94

GC content: 47.72

Gene sequence:

>417_bases
GTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGCGCTGTTCAAGCGCTTTACTCTTGGCAGTTGTCTAAAAACGACAT
CGCTGATGTTGAATTACAGTTCCTGTCGGAGCAGGATGTCAAAGATGTAGACATCGCCTATTTTCGCGAATTGCTGTCAG
GGGTTGCGGTTAATGCAGCATCTCTGGATGCATTAATGGCACCTTTTCTTTCCCGTCAGCTCGAAGAATTAGGTCAGGTT
GAACGTGCTGTTTTGCGTATTGCTCTGTTTGAACTGAGCAAACGTGATGATGTCCCTTATAAGGTGGCAATCAATGAAGC
GATCGAACTGGCCAAAACTTTCGGCGCGGAAGATAGCCATAAGTTCGTTAACGGAGTGCTGGATAAAGTCGCTCCGACGG
TACGTAAGAGAAAATAA

Upstream 100 bases:

>100_bases
CTAAAGCAGGTAATAAAGGTGCAGAAGCTGCCCTGACCGCGCTTGAAATGATTAATGTAATCAAAGCTATTAAAGGCTGA
ATTTAGTTAAGGGGAATTCC

Downstream 100 bases:

>100_bases
TCTCGTGATATCCCACGTCATTGGTGTTGCAGCCAGGCAGTAATCGAGGAAATCTCGTTGAGCTGACCCCGTCAGTGATT
CGGGTGACAAATCTGCCAAG

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 138; Mature: 138

Protein sequence:

>138_residues
MKPAARRRARECAVQALYSWQLSKNDIADVELQFLSEQDVKDVDIAYFRELLSGVAVNAASLDALMAPFLSRQLEELGQV
ERAVLRIALFELSKRDDVPYKVAINEAIELAKTFGAEDSHKFVNGVLDKVAPTVRKRK

Sequences:

>Translated_138_residues
MKPAARRRARECAVQALYSWQLSKNDIADVELQFLSEQDVKDVDIAYFRELLSGVAVNAASLDALMAPFLSRQLEELGQV
ERAVLRIALFELSKRDDVPYKVAINEAIELAKTFGAEDSHKFVNGVLDKVAPTVRKRK
>Mature_138_residues
MKPAARRRARECAVQALYSWQLSKNDIADVELQFLSEQDVKDVDIAYFRELLSGVAVNAASLDALMAPFLSRQLEELGQV
ERAVLRIALFELSKRDDVPYKVAINEAIELAKTFGAEDSHKFVNGVLDKVAPTVRKRK

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=138, Percent_Identity=84.0579710144928, Blast_Score=226, Evalue=3e-61,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 15506; Mature: 15506

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAARRRARECAVQALYSWQLSKNDIADVELQFLSEQDVKDVDIAYFRELLSGVAVNAA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
SLDALMAPFLSRQLEELGQVERAVLRIALFELSKRDDVPYKVAINEAIELAKTFGAEDSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHH
KFVNGVLDKVAPTVRKRK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPAARRRARECAVQALYSWQLSKNDIADVELQFLSEQDVKDVDIAYFRELLSGVAVNAA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHH
SLDALMAPFLSRQLEELGQVERAVLRIALFELSKRDDVPYKVAINEAIELAKTFGAEDSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHH
KFVNGVLDKVAPTVRKRK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA