Click here to switch to the map view.

The map label for this gene is thiL [H]

Identifier: 170025472

GI number: 170025472

Start: 3559482

End: 3560471

Strand: Reverse

Name: thiL [H]

Synonym: YPK_3255

Alternate gene names: 170025472

Gene position: 3560471-3559482 (Counterclockwise)

Preceding gene: 170025473

Following gene: 170025471

Centisome position: 75.93

GC content: 49.29

Gene sequence:

>990_bases
ATGGCATGTGGCGAATTTGACCTCATTGCCCGCTACTTTGACCGGTTTAGAAGTAACCGCCGGGATGTGGAGCTGGGTAT
TGGAGACGACTGCGCACTTCTTACAGTGGCAGAGAAACAGCTGTTGGCCATCAGTACCGATACACTGGTGTCCGGTGTTC
ATTTCCTTCCGGATATCGATCCGGCCGATCTTGGTTACAAATCTCTAGCGGTAAACCTCAGCGATTTAGCTGCGATGGGT
GCCGATCCTGCCTGGTTATCGCTTGCTTTGACTCTGCCATCGGTAAATGAAGATTGGCTACAGGCATTCAGTGACAGCTT
ATTTGATCAACTCAATTACTATGGTATGCAATTGATCGGTGGTGATACCACCCGTGGGCCATTGAGTCTGACACTGACTA
TTCACGGTTTTGTCCCCGAAGGCCGTGCATTGGCCCGTGCTGGTGCGCGGATTGGTGACTGGATCTATGTTACTGGCACA
TTGGGTGACAGTGCTGCGGGTCTGGCTATTTTGCAAAATGAGCTGCGGGTAGATAACGAAACCGATCGAGCGGCATTAAT
TCATCGTCATTTACGACCACAACCCCGAGTTTTACAGGGGCAAGCATTGCGTATGCTTGCTAGTGCTGCCATTGATATTT
CTGATGGGTTGATTTCTGATCTGCAGCACATTCTGAAAGCCAGCCATTGTGGTGCTCGTATTGAGCTCGACATGTTGCCG
TATTCCGATGCATTAAAAAATCAGGTTGATACTGAGCAAGCTCTCCGCTGGGCATTGAGTGGGGGTGAAGACTATGAACT
GTGCTTTACCGTACCCGAGATCAACCGGGGCGCGTTGGAAGTGGCACTCAGTAATACCGGAGCGGGTTATACCTGTATTG
GCCAAATAGGCCCTCAATCGGAAGGCGTTAAATTCTTCCGTGAAGGTAAAATAGTCAAACTGGATTTCCGCGGTTTTGAT
CATTTCTCAACAGGTAAATCTCATGGATGA

Upstream 100 bases:

>100_bases
TGCTGCAACGTCAAGATAGAAGGATATACATTAACGATATAAATTAAGGCCGGTATCCGGCCTTAATTTATATATCAGTT
AACATTTCTGGAATTGTATT

Downstream 100 bases:

>100_bases
AGCCAAGCGCCGTCTACGGTTGAGTAATCCCTGGCACTTGCTGGCGACCGGTTTTGGCAGTGGGCTATCACCATGGGCAC
CGGGTACTATGGGGTCAATT

Product: thiamine monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MACGEFDLIARYFDRFRSNRRDVELGIGDDCALLTVAEKQLLAISTDTLVSGVHFLPDIDPADLGYKSLAVNLSDLAAMG
ADPAWLSLALTLPSVNEDWLQAFSDSLFDQLNYYGMQLIGGDTTRGPLSLTLTIHGFVPEGRALARAGARIGDWIYVTGT
LGDSAAGLAILQNELRVDNETDRAALIHRHLRPQPRVLQGQALRMLASAAIDISDGLISDLQHILKASHCGARIELDMLP
YSDALKNQVDTEQALRWALSGGEDYELCFTVPEINRGALEVALSNTGAGYTCIGQIGPQSEGVKFFREGKIVKLDFRGFD
HFSTGKSHG

Sequences:

>Translated_329_residues
MACGEFDLIARYFDRFRSNRRDVELGIGDDCALLTVAEKQLLAISTDTLVSGVHFLPDIDPADLGYKSLAVNLSDLAAMG
ADPAWLSLALTLPSVNEDWLQAFSDSLFDQLNYYGMQLIGGDTTRGPLSLTLTIHGFVPEGRALARAGARIGDWIYVTGT
LGDSAAGLAILQNELRVDNETDRAALIHRHLRPQPRVLQGQALRMLASAAIDISDGLISDLQHILKASHCGARIELDMLP
YSDALKNQVDTEQALRWALSGGEDYELCFTVPEINRGALEVALSNTGAGYTCIGQIGPQSEGVKFFREGKIVKLDFRGFD
HFSTGKSHG
>Mature_328_residues
ACGEFDLIARYFDRFRSNRRDVELGIGDDCALLTVAEKQLLAISTDTLVSGVHFLPDIDPADLGYKSLAVNLSDLAAMGA
DPAWLSLALTLPSVNEDWLQAFSDSLFDQLNYYGMQLIGGDTTRGPLSLTLTIHGFVPEGRALARAGARIGDWIYVTGTL
GDSAAGLAILQNELRVDNETDRAALIHRHLRPQPRVLQGQALRMLASAAIDISDGLISDLQHILKASHCGARIELDMLPY
SDALKNQVDTEQALRWALSGGEDYELCFTVPEINRGALEVALSNTGAGYTCIGQIGPQSEGVKFFREGKIVKLDFRGFDH
FSTGKSHG

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=324, Percent_Identity=75.3086419753086, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 35738; Mature: 35607

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MACGEFDLIARYFDRFRSNRRDVELGIGDDCALLTVAEKQLLAISTDTLVSGVHFLPDID
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCEEEEEEHHHHHHCHHHCCCCC
PADLGYKSLAVNLSDLAAMGADPAWLSLALTLPSVNEDWLQAFSDSLFDQLNYYGMQLIG
CCCCCHHHEEEEHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEC
GDTTRGPLSLTLTIHGFVPEGRALARAGARIGDWIYVTGTLGDSAAGLAILQNELRVDNE
CCCCCCCEEEEEEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCC
TDRAALIHRHLRPQPRVLQGQALRMLASAAIDISDGLISDLQHILKASHCGARIELDMLP
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
YSDALKNQVDTEQALRWALSGGEDYELCFTVPEINRGALEVALSNTGAGYTCIGQIGPQS
CHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCC
EGVKFFREGKIVKLDFRGFDHFSTGKSHG
CCCHHHHCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ACGEFDLIARYFDRFRSNRRDVELGIGDDCALLTVAEKQLLAISTDTLVSGVHFLPDID
CCCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCEEEEEEHHHHHHCHHHCCCCC
PADLGYKSLAVNLSDLAAMGADPAWLSLALTLPSVNEDWLQAFSDSLFDQLNYYGMQLIG
CCCCCHHHEEEEHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEEEC
GDTTRGPLSLTLTIHGFVPEGRALARAGARIGDWIYVTGTLGDSAAGLAILQNELRVDNE
CCCCCCCEEEEEEEEEECCCCHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCC
TDRAALIHRHLRPQPRVLQGQALRMLASAAIDISDGLISDLQHILKASHCGARIELDMLP
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECC
YSDALKNQVDTEQALRWALSGGEDYELCFTVPEINRGALEVALSNTGAGYTCIGQIGPQS
CHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCEEEEECCCCCC
EGVKFFREGKIVKLDFRGFDHFSTGKSHG
CCCHHHHCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]