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The map label for this gene is ppnK

Identifier: 170025197

GI number: 170025197

Start: 3275347

End: 3276114

Strand: Direct

Name: ppnK

Synonym: YPK_2975

Alternate gene names: 170025197

Gene position: 3275347-3276114 (Clockwise)

Preceding gene: 170025195

Following gene: 170025198

Centisome position: 69.85

GC content: 46.74

Gene sequence:

>768_bases
ATGGTTGAACAACAGATTGCTCATGACCTCAATTTAACCGATGCCATAACAGGGAGTTTGGCAGATATTGGCCAAAAAGC
AGATTTGGCTGTTGTTGTCGGCGGTGATGGCAACATGCTCGGTGCTGCACGGGTACTTGCCCGTTATGATATAAAAGTGA
TTGGTGTCAACCGAGGTAATCTGGGTTTCTTGACCGATTTAGACCCGGATAATGCTCTGCAGCAGCTTTCAGACGTTTTG
GAAGGCGAGTACCTGAGTGAACAACGCTTCCTGCTAGAAACGCATGTACGACGTACAAATCAACAAAGCCGTATCAGTAC
CGCAATCAATGAAGTGGTTTTGCATCCAGGCAAAGTCGCCCATATGATCGAATTCGAAGTGTATATTGATGACCGCTTCG
CCTTTTCTCAACGCTCTGATGGCTTGATCATCGCAACCCCGACCGGTTCTACCGCCTATTCACTTTCCGCAGGCGGCCCA
ATCTTGACACCAACTCTGGATGCCATCGTATTGGTACCAATGTTTCCACACACTCTTACTGCGCGCCCATTGGTTATCAG
CAGCAGTAGCACCATCCGACTGAAGTTTTCGCACATTACCAGCGACCTGGAAATCAGTTGCGATAGCCAGATAGCCCTAC
CCATTCAGGAAGGGGAAGAGGTTTTAATCCGGCGCAGTGATTTTCACCTCAATCTTATTCATCCAAAGGACTATAGCTAT
TTCAATACGTTAAGCACCAAATTGGGTTGGTCAAAAAAATTATTCTAA

Upstream 100 bases:

>100_bases
ATTCGATTGTATTGGTATTGTCGGTCACCCGCGACACCCGGCTGCACTTGCCACCCATGAAATACTCTACCACTGGCTAA
AAGCCAGAGGCTACGCCGTT

Downstream 100 bases:

>100_bases
AAATCACCTCAGCTACTTTACTGTATATAAAACCAGTCTATACTGTATTCAAATACAGACATGTGTTTATGTACAGGGGG
TTTACCATGCTGGTCCAATT

Product: ATP-NAD/AcoX kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVL
EGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGP
ILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSDLEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSY
FNTLSTKLGWSKKLF

Sequences:

>Translated_255_residues
MVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVL
EGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGP
ILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSDLEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSY
FNTLSTKLGWSKKLF
>Mature_255_residues
MVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGNLGFLTDLDPDNALQQLSDVL
EGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVAHMIEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGP
ILTPTLDAIVLVPMFPHTLTARPLVISSSSTIRLKFSHITSDLEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSY
FNTLSTKLGWSKKLF

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=259, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788968, Length=255, Percent_Identity=84.7058823529412, Blast_Score=447, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6325068, Length=215, Percent_Identity=33.953488372093, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6320794, Length=245, Percent_Identity=30.2040816326531, Blast_Score=119, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6322509, Length=240, Percent_Identity=30, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI28573828, Length=253, Percent_Identity=28.0632411067194, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573826, Length=253, Percent_Identity=28.0632411067194, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573830, Length=253, Percent_Identity=28.0632411067194, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573832, Length=253, Percent_Identity=28.0632411067194, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161077047, Length=251, Percent_Identity=28.2868525896414, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI20129957, Length=276, Percent_Identity=24.6376811594203, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653424, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281363321, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI281363323, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24653422, Length=276, Percent_Identity=24.6376811594203, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_YERPE (Q8ZH09)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AB0136
- RefSeq:   NP_670373.1
- RefSeq:   YP_002346149.1
- ProteinModelPortal:   Q8ZH09
- SMR:   Q8ZH09
- IntAct:   Q8ZH09
- GeneID:   1148021
- GeneID:   1173954
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1106
- KEGG:   ypk:y3074
- KEGG:   ypm:YP_1050
- HOGENOM:   HBG713904
- OMA:   DGTMLNA
- ProtClustDB:   PRK03378
- BioCyc:   YPES187410:Y3074-MONOMER
- BioCyc:   YPES214092:YPO1106-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 28031; Mature: 28031

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGN
CCCHHHHHCCCCHHHHCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCC
LGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVA
EEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEE
HMIEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLT
EEEEEEEEECCEEEECCCCCCEEEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCC
ARPLVISSSSTIRLKFSHITSDLEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSY
CCEEEEECCCEEEEEEEECCCCEEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCH
FNTLSTKLGWSKKLF
HHHHHHHCCCCCCCC
>Mature Secondary Structure
MVEQQIAHDLNLTDAITGSLADIGQKADLAVVVGGDGNMLGAARVLARYDIKVIGVNRGN
CCCHHHHHCCCCHHHHCCCHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCEEEEEECCCC
LGFLTDLDPDNALQQLSDVLEGEYLSEQRFLLETHVRRTNQQSRISTAINEVVLHPGKVA
EEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEE
HMIEFEVYIDDRFAFSQRSDGLIIATPTGSTAYSLSAGGPILTPTLDAIVLVPMFPHTLT
EEEEEEEEECCEEEECCCCCCEEEEECCCCCEEEECCCCCEECCCCCEEEEEECCCCCCC
ARPLVISSSSTIRLKFSHITSDLEISCDSQIALPIQEGEEVLIRRSDFHLNLIHPKDYSY
CCEEEEECCCEEEEEEEECCCCEEEECCCEEEEECCCCCEEEEEECCCEEEEECCCCCCH
FNTLSTKLGWSKKLF
HHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430