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The map label for this gene is potH [H]

Identifier: 170024953

GI number: 170024953

Start: 3008186

End: 3009151

Strand: Reverse

Name: potH [H]

Synonym: YPK_2728

Alternate gene names: 170024953

Gene position: 3009151-3008186 (Counterclockwise)

Preceding gene: 170024954

Following gene: 170024952

Centisome position: 64.17

GC content: 50.0

Gene sequence:

>966_bases
GTGATACCAGAATCCACGAGTGGGGCCGCAGAACCCCCGATCCGCTCAGTTGGGCCGGTCAGGGCGCTGATACAACGCTT
CCAAATGGCGCATGGCCGTAAACTGGTCATCGCCATGCCATATTTATGGCTATTCCTCTTATTTATGCTGCCTTTCCTGA
TCGTGTTTAAAATCAGTTTGGCGGAAATGGTGCGGGCGGTTCCCCCTTATACCGATCTCACCACTTGGCTGGACGGCAAG
CTGGATATCTCGCTAAATCTGGGTAATTACCTGCTACTGCTGGATGATCCGTTATATATCGATGCCTATTTGCAATCGTT
GCAAGTGGCGGCGGTATCAACGCTGTGCTGCCTGCTTATTGGCTACCCATTGGCTTGGGCGATTGCTCACAGTAAACCAT
CGACCCGCAACATCTTATTGCTGTTGGTTATCTTGCCGTCATGGACGTCATTTCTGATCCGGGTTTATGCCTGGATGGGG
ATCCTAAAAAATAACGGCATCTTGAATAACTTTTTAATATGGACCGGAATTATTGATCAGCCGCTAGTCATCTTACACAC
CAATCTGGCCGTTTATATTGGCGTGGTGTATTCCTACCTGCCATTTATGGTGTTGCCGATTTATACCGCATTAACGCGAC
TGGATTACTCGCTGGTAGAAGCTGCGTTGGATCTCGGGGCCAGGCCGTTCAAGACGTTTATCAGTGTGATTATCCCGTTG
ACCAAAGGCGGTATTGTCGCTGGCTCTATGTTGGTATTTATCCCTGCAGTAGGGGAGTTTGTGATCCCCGAGCTCCTTGG
TGGGCCGGATAGCATCATGATTGGCCGCATTTTGTGGCAGGAGTTTTTCAATAATCGCGACTGGCCGGTGGCCTCTGCGG
TGGCAACCGTGATGTTAGTGCTGCTGATTGCCCCGATACTCTGGTTCCACAAACATCAAAATAAAGATATTGGGGGCGCG
AGATGA

Upstream 100 bases:

>100_bases
TAAAGGCATGCCAACGTGGGGTGATGAAGTGCAACTCTGTTGGGATGCTGACAGTTGCGTGGTTTTAGGGAGCTAATTAA
TTGTTTGGGGAGCCAATATT

Downstream 100 bases:

>100_bases
ATAACTTACCGGAGGTGCGTTCTGTCTGGCGTCGGGTGATCCTGACCGTCGGTTATACCTTCCTGTATGCCCCAATGTTG
ATGTTGGTAATTTATTCGTT

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MIPESTSGAAEPPIRSVGPVRALIQRFQMAHGRKLVIAMPYLWLFLLFMLPFLIVFKISLAEMVRAVPPYTDLTTWLDGK
LDISLNLGNYLLLLDDPLYIDAYLQSLQVAAVSTLCCLLIGYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMG
ILKNNGILNNFLIWTGIIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAALDLGARPFKTFISVIIPL
TKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFFNNRDWPVASAVATVMLVLLIAPILWFHKHQNKDIGGA
R

Sequences:

>Translated_321_residues
MIPESTSGAAEPPIRSVGPVRALIQRFQMAHGRKLVIAMPYLWLFLLFMLPFLIVFKISLAEMVRAVPPYTDLTTWLDGK
LDISLNLGNYLLLLDDPLYIDAYLQSLQVAAVSTLCCLLIGYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMG
ILKNNGILNNFLIWTGIIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAALDLGARPFKTFISVIIPL
TKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFFNNRDWPVASAVATVMLVLLIAPILWFHKHQNKDIGGA
R
>Mature_321_residues
MIPESTSGAAEPPIRSVGPVRALIQRFQMAHGRKLVIAMPYLWLFLLFMLPFLIVFKISLAEMVRAVPPYTDLTTWLDGK
LDISLNLGNYLLLLDDPLYIDAYLQSLQVAAVSTLCCLLIGYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMG
ILKNNGILNNFLIWTGIIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAALDLGARPFKTFISVIIPL
TKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFFNNRDWPVASAVATVMLVLLIAPILWFHKHQNKDIGGA
R

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=312, Percent_Identity=79.8076923076923, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1787369, Length=217, Percent_Identity=46.5437788018433, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787713, Length=231, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35760; Mature: 35760

Theoretical pI: Translated: 8.60; Mature: 8.60

Prosite motif: PS00133 CARBOXYPEPT_ZN_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPESTSGAAEPPIRSVGPVRALIQRFQMAHGRKLVIAMPYLWLFLLFMLPFLIVFKISL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AEMVRAVPPYTDLTTWLDGKLDISLNLGNYLLLLDDPLYIDAYLQSLQVAAVSTLCCLLI
HHHHHHCCCCCHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGILNNFLIWTGIIDQ
HHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAALDLGARPFKTFISVIIPL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFFNNRDWPVASAVATVMLV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLIAPILWFHKHQNKDIGGAR
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIPESTSGAAEPPIRSVGPVRALIQRFQMAHGRKLVIAMPYLWLFLLFMLPFLIVFKISL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AEMVRAVPPYTDLTTWLDGKLDISLNLGNYLLLLDDPLYIDAYLQSLQVAAVSTLCCLLI
HHHHHHCCCCCHHHHHCCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GYPLAWAIAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGILNNFLIWTGIIDQ
HHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEAALDLGARPFKTFISVIIPL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TKGGIVAGSMLVFIPAVGEFVIPELLGGPDSIMIGRILWQEFFNNRDWPVASAVATVMLV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLIAPILWFHKHQNKDIGGAR
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]