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The map label for this gene is potI [H]

Identifier: 170024952

GI number: 170024952

Start: 3007344

End: 3008189

Strand: Reverse

Name: potI [H]

Synonym: YPK_2727

Alternate gene names: 170024952

Gene position: 3008189-3007344 (Counterclockwise)

Preceding gene: 170024953

Following gene: 170024951

Centisome position: 64.15

GC content: 53.55

Gene sequence:

>846_bases
ATGAATAACTTACCGGAGGTGCGTTCTGTCTGGCGTCGGGTGATCCTGACCGTCGGTTATACCTTCCTGTATGCCCCAAT
GTTGATGTTGGTAATTTATTCGTTTAACAGCTCGAAGCTGGTGACGGTGTGGGCTGGGTGGTCAACCCGTTGGTATACGC
AGTTATTTAATGATTCGGCGATGATCTCTGCCGTTGGGCTGAGTCTGACGATTGCCGCCGCCTCTGCCACTATGGCGGTG
GTGTTGGGGACGATTGCCGCGGTGGTCATGGTGCGCTTTGGGCGTTTTCGTGGGTCAACCGGTTTTGCTTTTATGCTGAC
TGCACCGCTGGTTATGCCTGATGTCATCACCGGACTGTCACTACTGCTGCTGTTTGTGGCGATGGGGCATGCCATTGGGT
GGCCCACAGAACGTGGCATGTTCACCATCTGGCTGGCCCATGTGACTTTCTGCACGGCCTATGTGACGGTGGTCATCAGT
TCGCGGTTGCGCGAATTGGACCGTTCTATTGAAGAGGCGGCCATGGATCTGGGCGCGACACCACTGAAGGTCTTCTTTGT
TATTACTGTACCGATGATCGCCCCTGCGCTGATCTCTGGTTGGTTGCTGGCCTTTACCTTATCCCTTGATGATCTGGTTA
TTGCCAGCTTTGTTGCCGGGCCGGGGGCCACCACGTTACCGATGTTGGTATTCTCCAGTGTGCGTATGGGGGTGAATCCA
GAGATTAATGCGTTAGCGACACTTATCCTGCTGGTGGTTGGGGTTATCGGTTTATTTGCATGGTGGATAATGTCGCGTGC
GGAAAAGCAGAGATTGCGCGAATTACGTAAAGCTGCTCGTAGCTGA

Upstream 100 bases:

>100_bases
GGCCGGTGGCCTCTGCGGTGGCAACCGTGATGTTAGTGCTGCTGATTGCCCCGATACTCTGGTTCCACAAACATCAAAAT
AAAGATATTGGGGGCGCGAG

Downstream 100 bases:

>100_bases
TTCTCCCTAAAGCTGTTAGTCTTTCGTCTCCTTTAACGCCGCTTTAGAGCGGCGTTATTGTGAGAGATAACATCAGGTTG
CGGAAAACCGATTATGAGCA

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPTERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS

Sequences:

>Translated_281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPTERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
>Mature_281_residues
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSAMISAVGLSLTIAAASATMAV
VLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLSLLLLFVAMGHAIGWPTERGMFTIWLAHVTFCTAYVTVVIS
SRLRELDRSIEEAAMDLGATPLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=281, Percent_Identity=83.6298932384342, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1787368, Length=239, Percent_Identity=39.7489539748954, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787714, Length=256, Percent_Identity=33.59375, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=26.4150943396226, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30762; Mature: 30762

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCH
MISAVGLSLTIAAASATMAVVLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLLLFVAMGHAIGWPTERGMFTIWLAHVTFCTAYVTVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNNLPEVRSVWRRVILTVGYTFLYAPMLMLVIYSFNSSKLVTVWAGWSTRWYTQLFNDSA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCH
MISAVGLSLTIAAASATMAVVLGTIAAVVMVRFGRFRGSTGFAFMLTAPLVMPDVITGLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LLLLFVAMGHAIGWPTERGMFTIWLAHVTFCTAYVTVVISSRLRELDRSIEEAAMDLGAT
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLKVFFVITVPMIAPALISGWLLAFTLSLDDLVIASFVAGPGATTLPMLVFSSVRMGVNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
EINALATLILLVVGVIGLFAWWIMSRAEKQRLRELRKAARS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]