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The map label for this gene is metG [H]

Identifier: 170024779

GI number: 170024779

Start: 2801333

End: 2803360

Strand: Direct

Name: metG [H]

Synonym: YPK_2554

Alternate gene names: 170024779

Gene position: 2801333-2803360 (Clockwise)

Preceding gene: 170024774

Following gene: 170024781

Centisome position: 59.74

GC content: 48.32

Gene sequence:

>2028_bases
ATGGCTCAAGTCGCGAAAAAAATATTGGTGACGTGCGCGCTACCATACGCTAACGGTTCTATTCATCTCGGCCACATGCT
CGAGCATATCCAGGCAGACATCTGGGTCCGTTTCCAGCGAATGCGCGGCAACCAGGTTCATTTTATCTGTGCAGATGATG
CCCACGGGACTCCTATCATGCTGAAAGCTCAGCAAATGGGTATTGAACCAGAGCAGATGATTGCCGAAATGAGCCAGGAG
CACCAACAGGATTTCGCCGGTTTTGCTATCAGTTATGATAACTATCACTCCACCCACAGTGATGAAAACCGTGAACTGTC
GAGCCTGATCTATGGCCGTCTGAAAGCCAACGGTTATATAAAGAACCGTACTATTTCTCAGCTCTATGATCCTGAGAAAG
GCATGTTTCTGCCCGATCGTTTCGTGAAAGGCACTTGCCCAAAATGCAAAGCCCCGGAACAGTACGGCGATAACTGTGAA
GTCTGTGGCGCAACCTACAGCCCAACTGAACTTATCGACCCCAAATCCGCCGTTTCTGGTGCGACACCAGTGATGCGTGA
ATCTGAACACTTCTTCTTCGATTTACCGGCTTTCAGTGACATGTTGCAAGCCTGGACACGCTCAGGTGCGCTGCAAGAAC
AAGTCGCAAACAAAATGCAGGAATGGTTTGATTCCGGCCTGCAACAGTGGGATATCACGCGTGATGCCCCTTATTTCGGT
TTCGAAGTACCGGATGCACCGGGTAAATATTTTTACGTCTGGTTAGATGCCCCAATCGGTTACATGGGGGCGTTTAAAAA
CCTATGTGATAAACGTGGTGACCTGGATTTTGATGAATTCTGGGGTAAAGACGCCAAAACTGACCTCTATCACTTCATCG
GTAAAGATATTGTTTACTTCCACAGTCTGTTCTGGCCAGCCATGTTGGAAGGCAGTAACTTCCGCAAGCCGACAAACCTG
TTTGTCCATGGCTATGTCACGGTAAACGGCGCGAAAATGTCTAAATCTCGCGGTACGTTTATTAAGGCGGGGACTTACCT
GAAATATCTGGATGCGGATTGCCTGCGTTACTACTACGCGGCGAAACTCTCTTCGCGTATTGATGATATCGATTTAAATC
TGGAAGATTTCGTCCAACGCGTTAACGTCGATATCGTTAATAAAGTGGTGAATCTGGCCTCACGTAATGCTGGTTTTATC
AATAAACGCTTTGCAGGCCAGTTAGCTGACCAATTGGCTGACCCGGTGTTGTATAAAACATTCACCGATGCCGCAACCAG
TATTGCTGATGCTTATAACAACCGTGAGTCAGGTAAAGCCATTCGTGAAATTATGGCGCTAGCTGATGTGGCTAATCGCT
ATGTTGATGAACAGGCTCCATGGGTGGTCGCTAAACAAGAAGGCCGCGATGCCGATCTACACGCTATCTGCTCGATGGGG
ATCAACCTATTCCGTGTCCTGATGACCTATCTGAAACCGGTATTACCCTCTCTGACAGAGCGTACCGAGGCCTTCCTCAA
TACCGAGCTAACTTGGGATAGCATTGAACAACCTCTGCTGGGCCACTCTATCACCGCTTTCAAGGCACTGTTTAATCGTA
TTGATTTAGACAAAGTAAACGAAATGGTCGCCTCGTCTAAAGAAGATATGGCACCTGCAACCAGGGTTACAGGCCCGTTA
GCCGATGATCCAATTCAGGAAACCATCAGTTTTGATGACTTTGCCAAAGTTGATATGCGAATTGCGTTGATTCAACAAGC
CGAATTTGTTGAAGGCTCAGATAAGTTATTAAAACTAACACTTGAACTTGGAGGGGAGACCCGCCAGATATTCTCGGGTA
TCCGTTCAGCTTACCCTGACCCAAAAGCCTTGGAAGGCCGCATGACCGTGATGGTCGCTAACCTTGCCCCGAGAAAAATG
CGCTTTGGGGTTTCAGAAGGCATGGTGATAGCCGCAGGCCCCGGAGGTAGCGATATCTTCCTGCTTAGCCCTGACAGTGG
TGCGCAGCCAGGCATGCAAGTGAAGTGA

Upstream 100 bases:

>100_bases
ACTTAAGGTGCAGTACCCGCTCCCTTGCCCTAGCGGCAAACTCGCTTATCGGTTAACATCAAAGGCCCTTTAATCAACAT
AAAGAAAGCAATTTCCCACT

Downstream 100 bases:

>100_bases
TTTTCTTCTGGCGACTAAGCGAAGATACAATAGATAACTGAAAGATCCTTAAAGAACAAAATACCGGTCTGGGGTAACCC
TACCGGCATTTTTATGATTG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 675; Mature: 674

Protein sequence:

>675_residues
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQE
HQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCE
VCGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNVDIVNKVVNLASRNAGFI
NKRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPL
ADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQIFSGIRSAYPDPKALEGRMTVMVANLAPRKM
RFGVSEGMVIAAGPGGSDIFLLSPDSGAQPGMQVK

Sequences:

>Translated_675_residues
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQE
HQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCE
VCGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNL
FVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNVDIVNKVVNLASRNAGFI
NKRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPL
ADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQIFSGIRSAYPDPKALEGRMTVMVANLAPRKM
RFGVSEGMVIAAGPGGSDIFLLSPDSGAQPGMQVK
>Mature_674_residues
AQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIMLKAQQMGIEPEQMIAEMSQEH
QQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEV
CGATYSPTELIDPKSAVSGATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFGF
EVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLF
VHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYAAKLSSRIDDIDLNLEDFVQRVNVDIVNKVVNLASRNAGFIN
KRFAGQLADQLADPVLYKTFTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMGI
NLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVNEMVASSKEDMAPATRVTGPLA
DDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLTLELGGETRQIFSGIRSAYPDPKALEGRMTVMVANLAPRKMR
FGVSEGMVIAAGPGGSDIFLLSPDSGAQPGMQVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=566, Percent_Identity=27.5618374558304, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI24308436, Length=513, Percent_Identity=25.9259259259259, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1788432, Length=677, Percent_Identity=84.3426883308715, Blast_Score=1224, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=572, Percent_Identity=28.8461538461538, Blast_Score=229, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=23.8747553816047, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=23.4567901234568, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=28.8014311270125, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6321610, Length=538, Percent_Identity=22.4907063197026, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24655311, Length=570, Percent_Identity=28.5964912280702, Blast_Score=231, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358483, Length=513, Percent_Identity=24.5614035087719, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75661; Mature: 75530

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIM
CHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQMGIEPEQMIAEMSQEHQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEVCGATYSPTELIDPKSAVSG
ECCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCCHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNVDIVNKVVNLASRNAGFINKRFAGQLADQLADPVLYKT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
FTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCHHHHHHHHHHHCCHHHHH
EMVASSKEDMAPATRVTGPLADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLT
HHHHCCHHHCCCHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LELGGETRQIFSGIRSAYPDPKALEGRMTVMVANLAPRKMRFGVSEGMVIAAGPGGSDIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC
>Mature Secondary Structure 
AQVAKKILVTCALPYANGSIHLGHMLEHIQADIWVRFQRMRGNQVHFICADDAHGTPIM
HHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEE
LKAQQMGIEPEQMIAEMSQEHQQDFAGFAISYDNYHSTHSDENRELSSLIYGRLKANGYI
EEHHHHCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEE
KNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPEQYGDNCEVCGATYSPTELIDPKSAVSG
ECCCHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCHHHCCCHHHCCC
ATPVMRESEHFFFDLPAFSDMLQAWTRSGALQEQVANKMQEWFDSGLQQWDITRDAPYFG
CCCCCCCCCCEEEECHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
FEVPDAPGKYFYVWLDAPIGYMGAFKNLCDKRGDLDFDEFWGKDAKTDLYHFIGKDIVYF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHH
HSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKMSKSRGTFIKAGTYLKYLDADCLRYYYA
HHHHHHHHHCCCCCCCCCEEEEEEEEEEECCEECCCCCCEEECCCHHHHHHHHHHHHHHH
AKLSSRIDDIDLNLEDFVQRVNVDIVNKVVNLASRNAGFINKRFAGQLADQLADPVLYKT
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
FTDAATSIADAYNNRESGKAIREIMALADVANRYVDEQAPWVVAKQEGRDADLHAICSMG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHH
INLFRVLMTYLKPVLPSLTERTEAFLNTELTWDSIEQPLLGHSITAFKALFNRIDLDKVN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHCCHHHHHHHHHHHCCHHHHH
EMVASSKEDMAPATRVTGPLADDPIQETISFDDFAKVDMRIALIQQAEFVEGSDKLLKLT
HHHHCCHHHCCCHHHCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LELGGETRQIFSGIRSAYPDPKALEGRMTVMVANLAPRKMRFGVSEGMVIAAGPGGSDIF
ECCCCCHHHHHHHHHHCCCCCHHCCCEEEEEEECCCCHHHHCCCCCCEEEEECCCCCEEE
LLSPDSGAQPGMQVK
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA