Click here to switch to the map view.

The map label for this gene is astE [H]

Identifier: 170024454

GI number: 170024454

Start: 2466821

End: 2467813

Strand: Reverse

Name: astE [H]

Synonym: YPK_2225

Alternate gene names: 170024454

Gene position: 2467813-2466821 (Counterclockwise)

Preceding gene: 170024455

Following gene: 170024453

Centisome position: 52.62

GC content: 48.34

Gene sequence:

>993_bases
ATGCTGGATTTTTTAGCGATAACCCTATCGGGTAAACCTCCACAAGTCATACAGGGTGAAACCGTTAATCTGAAATGGCA
ATGGCTCGGTGAGGGGATATTGACACTGGTTCCGCACCGCAGCTACACACAATCGGTGGTGATCTCTGCCGGTATCCACG
GCAATGAAACGGCACCCATTGAAATTTTGAATCAATTAGTCACCGATTTATTAGCGGGTCAACTGACGCTGGCCGTCCGT
CTGTTAGTGTTATTGGGAAATCCACCGGCGATCCGCAAAGGGAAGCGGTACCTCAGCAATGACATTAACCGTATGTTTGG
TGGGCGTTATCAGCACTATACACCCAGTGACGAGACGCGAAGGGCGAGTACTCTGGAGCAACGGGTGATGGCGTTTTTCC
AGGCAAGTCACACATCAGAGCGTTTGCATTATGATCTACACACCGCAATTCGGGGGTCCTATCATCCTCGCTTTGGTTTG
TTACCCTACCAGCAGACCCCTTATTCCGCTGCTATGTTTCGTTGGTTGCGGGACATCGAACTGGACGCTTTGGTTATGCA
CACCTCCGCAGGAGGGACATTCGCTCACTTTAGCAGTGAGCGCTGTCAGGCAGCCAGTTGTACGTTGGAGTTAGGCAAAG
CATTACCTTTTGGTGAGAATCAATTATCACAATTCAGTGCGATCACCCAAGGATTACGCTCCTTAGTCAGTGATAGTGCC
TTGCCTGCACGTAAGACAGAGAACATGAAGTACTACCGGGTCGTTAAATCACTGCTTAGGCAACACCCTGATTTTAAACT
ACGGGGGGCTGAGGATACGGTAAATTTCACACGCTTCGCACAAGGCACTCTACTTACAGAACAACCCAATGATAACTATC
GTGTTGAACACCCCTATGAGTGGATCTTATTTCCAAATCCACATGTTGCGTTGGGATTACGGGCTGGGATGATGTTGGTA
AAAATGTGTGAAAGTGAATTACCGATTACATGA

Upstream 100 bases:

>100_bases
CCTCAATCTGGGGCCGGTGTACCCGTTCCAGCGCAATTAGCCGGTGTGGCTTTAGCGAATAACCGAACGGCTCAATGATA
GAACCAATAGAGGAGCGATG

Downstream 100 bases:

>100_bases
GCTTGTTGGGGCCAATACCGATGTGGGCGGTTCTCTGGAGTTATACCCATACAAATAGTAATAATTAATCTAATTTCAAA
TGAACTGTCTTGACGGTTCG

Product: succinylglutamate desuccinylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLTLAVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRGAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT

Sequences:

>Translated_330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLTLAVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRGAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT
>Mature_330_residues
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPIEILNQLVTDLLAGQLTLAVR
LLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETRRASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGL
LPYQQTPYSAAMFRWLRDIELDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
LPARKTENMKYYRVVKSLLRQHPDFKLRGAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYEWILFPNPHVALGLRAGMMLV
KMCESELPIT

Specific function: Transforms N(2)-succinylglutamate into succinate and glutamate [H]

COG id: COG2988

COG function: function code E; Succinylglutamate desuccinylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788040, Length=322, Percent_Identity=48.7577639751553, Blast_Score=319, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007036
- InterPro:   IPR016681 [H]

Pfam domain/function: PF04952 AstE_AspA [H]

EC number: =3.5.1.96 [H]

Molecular weight: Translated: 37214; Mature: 37214

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPI
CCEEEEEEECCCCCCEECCCEEEEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCCHH
EILNQLVTDLLAGQLTLAVRLLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGLLPYQQTPYSAAMFRWLRDIE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
LDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
CCEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPARKTENMKYYRVVKSLLRQHPDFKLRGAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYE
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHCCEEEECCCCCCCEECCCEE
WILFPNPHVALGLRAGMMLVKMCESELPIT
EEEECCCCEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDFLAITLSGKPPQVIQGETVNLKWQWLGEGILTLVPHRSYTQSVVISAGIHGNETAPI
CCEEEEEEECCCCCCEECCCEEEEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCCHH
EILNQLVTDLLAGQLTLAVRLLVLLGNPPAIRKGKRYLSNDINRMFGGRYQHYTPSDETR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RASTLEQRVMAFFQASHTSERLHYDLHTAIRGSYHPRFGLLPYQQTPYSAAMFRWLRDIE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
LDALVMHTSAGGTFAHFSSERCQAASCTLELGKALPFGENQLSQFSAITQGLRSLVSDSA
CCEEEEEECCCCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LPARKTENMKYYRVVKSLLRQHPDFKLRGAEDTVNFTRFAQGTLLTEQPNDNYRVEHPYE
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHCCEEEECCCCCCCEECCCEE
WILFPNPHVALGLRAGMMLVKMCESELPIT
EEEECCCCEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA