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The map label for this gene is phoE [H]

Identifier: 170024453

GI number: 170024453

Start: 2464981

End: 2466063

Strand: Reverse

Name: phoE [H]

Synonym: YPK_2224

Alternate gene names: 170024453

Gene position: 2466063-2464981 (Counterclockwise)

Preceding gene: 170024454

Following gene: 170024449

Centisome position: 52.59

GC content: 44.51

Gene sequence:

>1083_bases
ATGAAAAACATCGCGATGGCTTTGGCAGCCACACTGGGTATCATTCCAGCTCTTTCAAGTGCAGCGGAAATTTATAATAA
AGATGGCAACAAGTTGGATTTTTATGGGCGGGTTGCTGCGAAATACCTATTTAAAAAACAGAGCAATGAAGATGCAACTT
ATGTGCGTATTGGTTTCAAGGGTGAAACAAAAATAAATAGCCAGCTAACTGGTTTCGGTCAGTGGGAATATAACATCGCA
GCTAACAACACAGAATCTCAGGGTGATAAGGGCAATAAAACCCGTTTGGGCTTTGCGGGTCTGAAGTTAGCTGATTACGG
GTCTTTCGACTATGGCCGCAACTATGGTGTTGTCTATGACGTGCTGTCCTATACCGATATGTTGCCTGAATTTGGTGGTG
ATTCCATGGCAGCGACGGATAATTACATGACGGCCCGCTCTACTGGCCTGGCAACCTACCGTAACGATAATTTCTTCGGA
CTTGTGGAGGGCTTAAAAGTTGCCGCTCAGTATCAGGGGCGTAACGAAGACGGTGATGTTACCCGAAATGAACGTTCTAT
CCAAAAGGCCAATGGTGATGGTTTCGGTTTGTCTTTTAATTATCAAAACATTCAGGGCAGCGGCGTCAGTTTTGCAGGCG
CATACTCATCATCTAATCGTACCGAGGGCCAGAAAGAATTAGTGAACAGTGCTGCGGGTGACAAAGCTCAAGCTTGGGCT
ACTGGGCTAAAATACGATGCCAATCAGGCCTATATTGCAGCAATGTATGGCGAAACACTAAACATGACGCCGTACAAAAG
ACTGATCGCGAACAAAACCCAAAACGTAGAGTTGGTCGCTCAATACCAGTTTGAGAATGGTATCCGCCCGTCTCTTGCCT
ACATACAATCTAAAGGTAAGGATCTGGCGGGGGTCGGCGATACGGATCTGGTGAAATATGCTGAAATCGGTGTGACTTAC
CATTTCAACAAGAACATGTACACCTATGTTGATTATAAGATTAATTTATTGGATAACGATAAGAACCCATTGGGCTTGAA
TACGGATGATGTTGTTGCGGTAAACTTGACTTACCGTTTTTAA

Upstream 100 bases:

>100_bases
AACAAGACTTAATTCTCACAGCAAGACTTAATCCTCATCGTAAGATTAAACATTAACGAATTCTGAGGTTAATGTTATAA
AAATTAAAAGGAAGATATTA

Downstream 100 bases:

>100_bases
GCAGTAGATCTAAAGCGTTATGAATATTGGTGTTATTGCCTTTTTTCTGTGTCGGAAACAGGTGAAATAGAAAGATAGAA
CGGCGGGGGAAACCCCGCCT

Product: porin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKNIAMALAATLGIIPALSSAAEIYNKDGNKLDFYGRVAAKYLFKKQSNEDATYVRIGFKGETKINSQLTGFGQWEYNIA
ANNTESQGDKGNKTRLGFAGLKLADYGSFDYGRNYGVVYDVLSYTDMLPEFGGDSMAATDNYMTARSTGLATYRNDNFFG
LVEGLKVAAQYQGRNEDGDVTRNERSIQKANGDGFGLSFNYQNIQGSGVSFAGAYSSSNRTEGQKELVNSAAGDKAQAWA
TGLKYDANQAYIAAMYGETLNMTPYKRLIANKTQNVELVAQYQFENGIRPSLAYIQSKGKDLAGVGDTDLVKYAEIGVTY
HFNKNMYTYVDYKINLLDNDKNPLGLNTDDVVAVNLTYRF

Sequences:

>Translated_360_residues
MKNIAMALAATLGIIPALSSAAEIYNKDGNKLDFYGRVAAKYLFKKQSNEDATYVRIGFKGETKINSQLTGFGQWEYNIA
ANNTESQGDKGNKTRLGFAGLKLADYGSFDYGRNYGVVYDVLSYTDMLPEFGGDSMAATDNYMTARSTGLATYRNDNFFG
LVEGLKVAAQYQGRNEDGDVTRNERSIQKANGDGFGLSFNYQNIQGSGVSFAGAYSSSNRTEGQKELVNSAAGDKAQAWA
TGLKYDANQAYIAAMYGETLNMTPYKRLIANKTQNVELVAQYQFENGIRPSLAYIQSKGKDLAGVGDTDLVKYAEIGVTY
HFNKNMYTYVDYKINLLDNDKNPLGLNTDDVVAVNLTYRF
>Mature_360_residues
MKNIAMALAATLGIIPALSSAAEIYNKDGNKLDFYGRVAAKYLFKKQSNEDATYVRIGFKGETKINSQLTGFGQWEYNIA
ANNTESQGDKGNKTRLGFAGLKLADYGSFDYGRNYGVVYDVLSYTDMLPEFGGDSMAATDNYMTARSTGLATYRNDNFFG
LVEGLKVAAQYQGRNEDGDVTRNERSIQKANGDGFGLSFNYQNIQGSGVSFAGAYSSSNRTEGQKELVNSAAGDKAQAWA
TGLKYDANQAYIAAMYGETLNMTPYKRLIANKTQNVELVAQYQFENGIRPSLAYIQSKGKDLAGVGDTDLVKYAEIGVTY
HFNKNMYTYVDYKINLLDNDKNPLGLNTDDVVAVNLTYRF

Specific function: Uptake of inorganic phosphate, phosphorylated compounds, and some other negatively charged solutes [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1787641, Length=359, Percent_Identity=57.9387186629526, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1786436, Length=363, Percent_Identity=55.6473829201102, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1787160, Length=374, Percent_Identity=54.2780748663102, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1788544, Length=373, Percent_Identity=52.2788203753351, Blast_Score=349, Evalue=1e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 39623; Mature: 39623

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIAMALAATLGIIPALSSAAEIYNKDGNKLDFYGRVAAKYLFKKQSNEDATYVRIGFK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEC
GETKINSQLTGFGQWEYNIAANNTESQGDKGNKTRLGFAGLKLADYGSFDYGRNYGVVYD
CCCEECCCEEECCEEEEEEECCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCHHHH
VLSYTDMLPEFGGDSMAATDNYMTARSTGLATYRNDNFFGLVEGLKVAAQYQGRNEDGDV
HHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCC
TRNERSIQKANGDGFGLSFNYQNIQGSGVSFAGAYSSSNRTEGQKELVNSAAGDKAQAWA
CCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHH
TGLKYDANQAYIAAMYGETLNMTPYKRLIANKTQNVELVAQYQFENGIRPSLAYIQSKGK
CCCEECCCCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCC
DLAGVGDTDLVKYAEIGVTYHFNKNMYTYVDYKINLLDNDKNPLGLNTDDVVAVNLTYRF
CCCCCCCHHHEEEEEEEEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEEEEC
>Mature Secondary Structure
MKNIAMALAATLGIIPALSSAAEIYNKDGNKLDFYGRVAAKYLFKKQSNEDATYVRIGFK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEC
GETKINSQLTGFGQWEYNIAANNTESQGDKGNKTRLGFAGLKLADYGSFDYGRNYGVVYD
CCCEECCCEEECCEEEEEEECCCCCCCCCCCCCEEEEEECEEEECCCCCCCCCCCCHHHH
VLSYTDMLPEFGGDSMAATDNYMTARSTGLATYRNDNFFGLVEGLKVAAQYQGRNEDGDV
HHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEECCCEEEHHHHHHHHHHCCCCCCCCCC
TRNERSIQKANGDGFGLSFNYQNIQGSGVSFAGAYSSSNRTEGQKELVNSAAGDKAQAWA
CCCCHHHHHCCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCHHHHH
TGLKYDANQAYIAAMYGETLNMTPYKRLIANKTQNVELVAQYQFENGIRPSLAYIQSKGK
CCCEECCCCEEEEEEECCCCCCCHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHCCC
DLAGVGDTDLVKYAEIGVTYHFNKNMYTYVDYKINLLDNDKNPLGLNTDDVVAVNLTYRF
CCCCCCCHHHEEEEEEEEEEEECCCEEEEEEEEEEEEECCCCCCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3032618 [H]