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The map label for this gene is mlc [H]

Identifier: 170024218

GI number: 170024218

Start: 2200949

End: 2202166

Strand: Reverse

Name: mlc [H]

Synonym: YPK_1981

Alternate gene names: 170024218

Gene position: 2202166-2200949 (Counterclockwise)

Preceding gene: 170024219

Following gene: 170024217

Centisome position: 46.96

GC content: 46.72

Gene sequence:

>1218_bases
GTGATAATGGATGGACAGCCTGGACATATTGATCAGATCAAACAAACCAATGTAGGGGCCGTTTATCGGCTAATTGATCT
TTTGGGGCCGATATCCCGTATTGAACTCTCCAAACGGGCCCAATTAGCGCCCGCCAGCATCACTAAAATTGTGCGAGAAT
TGGTCGAAGTTCATCTGGTAAAAGAGACTGAATATCAAGATGTAGGAAGCCGAGGTAGACCTGCTATTGGGTTAGTCTTG
GATACCGAGGCTTGGCACTATGTTTCCGGGCGTATCAGCCGTGGAACGATAACACTGGCACTGCGCGACCTTAGCAGCAA
ACTGGTGGTTGAAGAACAAATTCCGCTGCCAGACCGCCACCCCGAGCCACTGCTGAGCCGTATCCTTAACGAGGTTGACC
AGTTTTTTATTCGCCATCAGAAAAAACTCGAACGGCTCACCGCGATTGCTATTACGATGCCAGGCATCATCGATGCACCT
GCGGGTATTGTGCATAAAATGCCATTTTATGATGTTAATGAAATGTCACTTGGGCCAGCGTTGGAGCAACGCACCGGTTT
ACCTGTCTATCTGCAGCATGATATTTGCGCATGGACCATGGCAGAGTCGCTATATGGTGCCTCACGCGGTTGCCAGAATA
TCATTCAAGTCGTTATTGACCATAATGTCGGGGCCGGTGTTATCACCTCTGGGCGGGTATTACATGCTGGGAGTCGCAGT
GTCGTTAATATTGGCCATACTCAGGTTGACCCCTATGGTAAACGCTGCTATTGCGGCAATCATGGCTGTTTGGAAACCGT
TGCCAGTATCGATAATATGTTGGCGATTGCTCAACAACGATTAAACAGTTCAATGAGTTCGCTGTTGCACCATACACCTC
TCAGTGTTGAGTCGCTTTGTGATGCGGCGTTAGCCGGTGACCAATTAGCCAAAGATATTATTCTGGGTGTAGGTCATAGT
GTCGGGCGAATTATTGCCATTATGGTCAATTTATTTAATCCAGAGAAAATTCTGGTCGGGTCGCCATTGAATAAAGCATC
ATCTATTTTGCACCCGGCAATTGCCTCTTGTATTCGTCAACAAGCATTGCCCGCATATAGCGAGAATATTCTTGTTGAAT
CAACCGAATTTTTTAATCAAGGTACAATGCCTGGCGCAGCATTAGTCAAAGAGGCATTATACAATGGCTCATTATTAGTG
AAGTTGCTTCAAGGCTAG

Upstream 100 bases:

>100_bases
ACCAAAAGCCCCAACATAACGATAATCTTTATTTAGAAGCGCGAAAAATACTCTTTTACAAGAGTTAAAGAGTCATTTAG
GCAAAAAAGGAGCCGGTTTA

Downstream 100 bases:

>100_bases
TGTATTAAATCTAATAATTAAGAGTTAATTATTAGTGGGTACCTTCGGTCTATTACGCCTATTATTTGGCATTTTCTGTT
AAAAACACGCTCAGTTCGGC

Product: ROK family protein

Products: NA

Alternate protein names: Making large colonies protein [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIMDGQPGHIDQIKQTNVGAVYRLIDLLGPISRIELSKRAQLAPASITKIVRELVEVHLVKETEYQDVGSRGRPAIGLVL
DTEAWHYVSGRISRGTITLALRDLSSKLVVEEQIPLPDRHPEPLLSRILNEVDQFFIRHQKKLERLTAIAITMPGIIDAP
AGIVHKMPFYDVNEMSLGPALEQRTGLPVYLQHDICAWTMAESLYGASRGCQNIIQVVIDHNVGAGVITSGRVLHAGSRS
VVNIGHTQVDPYGKRCYCGNHGCLETVASIDNMLAIAQQRLNSSMSSLLHHTPLSVESLCDAALAGDQLAKDIILGVGHS
VGRIIAIMVNLFNPEKILVGSPLNKASSILHPAIASCIRQQALPAYSENILVESTEFFNQGTMPGAALVKEALYNGSLLV
KLLQG

Sequences:

>Translated_405_residues
MIMDGQPGHIDQIKQTNVGAVYRLIDLLGPISRIELSKRAQLAPASITKIVRELVEVHLVKETEYQDVGSRGRPAIGLVL
DTEAWHYVSGRISRGTITLALRDLSSKLVVEEQIPLPDRHPEPLLSRILNEVDQFFIRHQKKLERLTAIAITMPGIIDAP
AGIVHKMPFYDVNEMSLGPALEQRTGLPVYLQHDICAWTMAESLYGASRGCQNIIQVVIDHNVGAGVITSGRVLHAGSRS
VVNIGHTQVDPYGKRCYCGNHGCLETVASIDNMLAIAQQRLNSSMSSLLHHTPLSVESLCDAALAGDQLAKDIILGVGHS
VGRIIAIMVNLFNPEKILVGSPLNKASSILHPAIASCIRQQALPAYSENILVESTEFFNQGTMPGAALVKEALYNGSLLV
KLLQG
>Mature_405_residues
MIMDGQPGHIDQIKQTNVGAVYRLIDLLGPISRIELSKRAQLAPASITKIVRELVEVHLVKETEYQDVGSRGRPAIGLVL
DTEAWHYVSGRISRGTITLALRDLSSKLVVEEQIPLPDRHPEPLLSRILNEVDQFFIRHQKKLERLTAIAITMPGIIDAP
AGIVHKMPFYDVNEMSLGPALEQRTGLPVYLQHDICAWTMAESLYGASRGCQNIIQVVIDHNVGAGVITSGRVLHAGSRS
VVNIGHTQVDPYGKRCYCGNHGCLETVASIDNMLAIAQQRLNSSMSSLLHHTPLSVESLCDAALAGDQLAKDIILGVGHS
VGRIIAIMVNLFNPEKILVGSPLNKASSILHPAIASCIRQQALPAYSENILVESTEFFNQGTMPGAALVKEALYNGSLLV
KLLQG

Specific function: Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI1787878, Length=406, Percent_Identity=73.6453201970443, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1786891, Length=403, Percent_Identity=42.4317617866005, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI87082127, Length=334, Percent_Identity=23.0538922155689, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: NA

Molecular weight: Translated: 44035; Mature: 44035

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMDGQPGHIDQIKQTNVGAVYRLIDLLGPISRIELSKRAQLAPASITKIVRELVEVHLV
CCCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KETEYQDVGSRGRPAIGLVLDTEAWHYVSGRISRGTITLALRDLSSKLVVEEQIPLPDRH
HCCCHHHCCCCCCCEEEEEEECCCHHHHCCEECCCEEEEEEECCCCCEEEEECCCCCCCC
PEPLLSRILNEVDQFFIRHQKKLERLTAIAITMPGIIDAPAGIVHKMPFYDVNEMSLGPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCCCCCCCCH
LEQRTGLPVYLQHDICAWTMAESLYGASRGCQNIIQVVIDHNVGAGVITSGRVLHAGSRS
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCC
VVNIGHTQVDPYGKRCYCGNHGCLETVASIDNMLAIAQQRLNSSMSSLLHHTPLSVESLC
EEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DAALAGDQLAKDIILGVGHSVGRIIAIMVNLFNPEKILVGSPLNKASSILHPAIASCIRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
QALPAYSENILVESTEFFNQGTMPGAALVKEALYNGSLLVKLLQG
HCCCCCCCCEEEECHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MIMDGQPGHIDQIKQTNVGAVYRLIDLLGPISRIELSKRAQLAPASITKIVRELVEVHLV
CCCCCCCCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
KETEYQDVGSRGRPAIGLVLDTEAWHYVSGRISRGTITLALRDLSSKLVVEEQIPLPDRH
HCCCHHHCCCCCCCEEEEEEECCCHHHHCCEECCCEEEEEEECCCCCEEEEECCCCCCCC
PEPLLSRILNEVDQFFIRHQKKLERLTAIAITMPGIIDAPAGIVHKMPFYDVNEMSLGPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHCCCCCCCCCCCCCCH
LEQRTGLPVYLQHDICAWTMAESLYGASRGCQNIIQVVIDHNVGAGVITSGRVLHAGSRS
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCEEEECCCCC
VVNIGHTQVDPYGKRCYCGNHGCLETVASIDNMLAIAQQRLNSSMSSLLHHTPLSVESLC
EEECCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
DAALAGDQLAKDIILGVGHSVGRIIAIMVNLFNPEKILVGSPLNKASSILHPAIASCIRQ
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHH
QALPAYSENILVESTEFFNQGTMPGAALVKEALYNGSLLVKLLQG
HCCCCCCCCEEEECHHHHCCCCCCHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7766024; 9097039; 9278503 [H]