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The map label for this gene is bioD2

Identifier: 170024217

GI number: 170024217

Start: 2200057

End: 2200725

Strand: Reverse

Name: bioD2

Synonym: YPK_1980

Alternate gene names: 170024217

Gene position: 2200725-2200057 (Counterclockwise)

Preceding gene: 170024218

Following gene: 170024211

Centisome position: 46.93

GC content: 43.35

Gene sequence:

>669_bases
ATGTTAACGCGTTTATTTGTGACAGGTACCGACACTGCTGTTGGTAAAACCGTCGTGTCACGGGCTTTACTTCAAGCCTT
AAGCCAAAATGGCAGAACCGCAGTAGGCTATAAACCTGTTGCGACAGAAAGCAAAGAGACCTCGGAAGGGTTGCGTAATC
AAGATGCGTTGATCTTGCAGGCGTCATCATCCATTGAGTTAAATTATCAGGAGGTAAATCCATATCCATTACAAGGTGAT
GTTATTCATGCCTGTACAGATACCCTGATTAATTATGAAAAGATGACCGAAGGATTGCAGTGTTTATCGGCTAAAGCAGA
TACCGTAATCGTTGAAGGCTGCGGTGGGTGGAAGGTTATGATGAATGACCAACGCTTTTACTCTGACTGGGTGGTACAAG
AACAATTACCCGTTATTTTAGTCGTCGGAATTAAATTAGGCTGTATCAATCACGCCTTATTAACAGCACAGGCAATTATT
AATGATGGGTTGCCCTTATTGGGTTGGGTTGCCAATCGCATTAATCCTGGCTTGGCCCATTATGCTGAAACAATCGCAAT
GCTACGGGATCGGTTAGCCGCGCCACAATTAGGGCAACTGCCTTATTTGCCGCGACCAGAAGAAAAACCATTAGCAAAAT
ATTTAGATCTTACAGCTATTTCAGGATAA

Upstream 100 bases:

>100_bases
GTCTGTTGCTGTCATCGCCTAGACTTTTATTCATATTGCCAATTCCGCCAACTTCGGTGGAAAAACTTGAGTTAAAGTGG
TCAGATTGCGGAGTGTTGTT

Downstream 100 bases:

>100_bases
CCCGATTTATATCCATCCTATTTCAAGCTGAATGTGTGTTTCAAGCTGGAATGTGTGTTTCAAGCTGAATGTATGTTGGC
TGCTTTCGTTACTCGGCCCA

Product: putative dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase 2; DTBS 2; Dethiobiotin synthase 2

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MLTRLFVTGTDTAVGKTVVSRALLQALSQNGRTAVGYKPVATESKETSEGLRNQDALILQASSSIELNYQEVNPYPLQGD
VIHACTDTLINYEKMTEGLQCLSAKADTVIVEGCGGWKVMMNDQRFYSDWVVQEQLPVILVVGIKLGCINHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIAMLRDRLAAPQLGQLPYLPRPEEKPLAKYLDLTAISG

Sequences:

>Translated_222_residues
MLTRLFVTGTDTAVGKTVVSRALLQALSQNGRTAVGYKPVATESKETSEGLRNQDALILQASSSIELNYQEVNPYPLQGD
VIHACTDTLINYEKMTEGLQCLSAKADTVIVEGCGGWKVMMNDQRFYSDWVVQEQLPVILVVGIKLGCINHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIAMLRDRLAAPQLGQLPYLPRPEEKPLAKYLDLTAISG
>Mature_222_residues
MLTRLFVTGTDTAVGKTVVSRALLQALSQNGRTAVGYKPVATESKETSEGLRNQDALILQASSSIELNYQEVNPYPLQGD
VIHACTDTLINYEKMTEGLQCLSAKADTVIVEGCGGWKVMMNDQRFYSDWVVQEQLPVILVVGIKLGCINHALLTAQAII
NDGLPLLGWVANRINPGLAHYAETIAMLRDRLAAPQLGQLPYLPRPEEKPLAKYLDLTAISG

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI87081944, Length=220, Percent_Identity=64.0909090909091, Blast_Score=286, Evalue=5e-79,
Organism=Escherichia coli, GI1786995, Length=222, Percent_Identity=51.8018018018018, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD2_YERPE (P69953)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0276
- RefSeq:   NP_669423.1
- RefSeq:   NP_993400.1
- RefSeq:   YP_002347235.1
- ProteinModelPortal:   P69953
- SMR:   P69953
- GeneID:   1147058
- GeneID:   1175100
- GeneID:   2764013
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2269
- KEGG:   ypk:y2111
- KEGG:   ypm:YP_2065
- HOGENOM:   HBG650065
- OMA:   INPGLSH
- ProtClustDB:   PRK12374
- BioCyc:   YPES187410:Y2111-MONOMER
- BioCyc:   YPES214092:YPO2269-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 24192; Mature: 24192

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLFVTGTDTAVGKTVVSRALLQALSQNGRTAVGYKPVATESKETSEGLRNQDALILQ
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCCCEEEEE
ASSSIELNYQEVNPYPLQGDVIHACTDTLINYEKMTEGLQCLSAKADTVIVEGCGGWKVM
ECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
MNDQRFYSDWVVQEQLPVILVVGIKLGCINHALLTAQAIINDGLPLLGWVANRINPGLAH
ECCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
YAETIAMLRDRLAAPQLGQLPYLPRPEEKPLAKYLDLTAISG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MLTRLFVTGTDTAVGKTVVSRALLQALSQNGRTAVGYKPVATESKETSEGLRNQDALILQ
CCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHCCCCCEEEEE
ASSSIELNYQEVNPYPLQGDVIHACTDTLINYEKMTEGLQCLSAKADTVIVEGCGGWKVM
ECCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
MNDQRFYSDWVVQEQLPVILVVGIKLGCINHALLTAQAIINDGLPLLGWVANRINPGLAH
ECCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHH
YAETIAMLRDRLAAPQLGQLPYLPRPEEKPLAKYLDLTAISG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430