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The map label for this gene is manB [H]

Identifier: 170023873

GI number: 170023873

Start: 1808677

End: 1810065

Strand: Reverse

Name: manB [H]

Synonym: YPK_1632

Alternate gene names: 170023873

Gene position: 1810065-1808677 (Counterclockwise)

Preceding gene: 170023874

Following gene: 170023872

Centisome position: 38.6

GC content: 46.36

Gene sequence:

>1389_bases
ATGGAAAAATTAACCTGTTTTAAAGCTTACGATATTCGGGGAAAACTGGGCTCGGAGTTAAACGAAGAGATAGCCTATCG
AATCGGACGCGCCTATTGCCAGTGGCTAAAACCAGCCAAAGTCGTGGTGGGTGGGGATGCACGGGCAACCAGTGTCGAAT
TAAAAAACGCCCTGGCAAAAGGGTTAATGGATGGCGGTGCGGATGTCATTGATATCGGCATGGTGGGGACTGAAGAAGTC
TATTTCGCCACCCGCTTTTATGGTGTCGATGGCGGGATTCAGGTGACGGCTAGCCATAATCCGATTGACTATAACGGGAT
GAAGTTAGTTAAAAAAGATGCCCAACCGATTAGCGGTGATAACGGGCTGTATGAAATTCAGCGGCTGGCACAGGATAATC
ATTTTGCGCCACCTGGCGTCAGGGGCCAGCTTGAAACCCAGTTGGTCCGCGAACACTATGTCGAGCACTTATTAAGTTAT
ATCGATCTGAATGCGTTACTGCCAATGAAAATTGTGGTTAATGCTGGCAACGGCGCGGCTGGCCCCACTCTTGATGCACT
GGAAGCTGCGCTGGCAAAACGTAAGGTGCCAATCAGTTTTATTAAAATCAACCATCAGCCAGATAGCAGTTTTCCTAACG
GGATCCCAAACCCGATGTTGCCAGAGAATCAGTGGTCAACTCGGCAGGCAGTTTGTGAGCATCAGGCAGATTTAGGCATT
GCCTGGGATGGGGATTTTGACCGTTGTTTCTTCTTCGACCACCTTGGGAATTTTATTGAGGGCTATTACATTGTGGGGCT
GCTGGCAGAAAAATTCCTGCTCACCAATAATATGGGTAAAGCGACTCACAATACTGACAAAATAATCCACGACCCTAGGT
TGACGTGGAATACGATAGATATCGTACAGACACTTAATGGTACAGCAATCGAATCTAAAACCGGCCATGCCTTTATTAAA
GATGCCATGCGCCACCATGATGCCGTCTATGGCGGGGAGATGAGCGCTCATCACTATTTCCGAGAGTTCGGCTATTGCGA
CAGCGGTATGATCCCTTGGCTGTTAGTCATCGAATTACTGTCGAAAAAAACCACGTCGCTGTTTTCATTAATTGAATCCC
GCAAGGCGAAATTCCCGGTATCGGGCGAACTTAATTTCACCGTTAGCGATCCAAAAACTAAAATCCAGCAGATAAAACAG
TTTTACGCTGATAGTGCCATCCACGTAACTGGAATTGATGGCCTTAGTATGGAATTTGAACACTGGCGTTTTAATTTACG
CTCATCGAATACTGAACCACTTGTGCGTTTAAATATTGAAACTCGTGGTGATGTCACACTTCTGGAGCAGAAAACAGCGC
AATTAACTCAACTGCTTTATCCAAAGTAA

Upstream 100 bases:

>100_bases
GAAGCCGCAAGGGCCCTTATTTCAGGCCAATTAGACAATGATATATCTTTAGATTTTGAACTTGTCGTCCGTGATTCAGC
ATAATCAGATTGGAAAGGAA

Downstream 100 bases:

>100_bases
TTAAAGTTGCAAATAACCTTTCGTTAAAAACTAATAAAATCAACCTCGCGTTCATTATTGAACGCGAGGGTAAATAGCAG
CATATTGTTGGAGCTTATAA

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MEKLTCFKAYDIRGKLGSELNEEIAYRIGRAYCQWLKPAKVVVGGDARATSVELKNALAKGLMDGGADVIDIGMVGTEEV
YFATRFYGVDGGIQVTASHNPIDYNGMKLVKKDAQPISGDNGLYEIQRLAQDNHFAPPGVRGQLETQLVREHYVEHLLSY
IDLNALLPMKIVVNAGNGAAGPTLDALEAALAKRKVPISFIKINHQPDSSFPNGIPNPMLPENQWSTRQAVCEHQADLGI
AWDGDFDRCFFFDHLGNFIEGYYIVGLLAEKFLLTNNMGKATHNTDKIIHDPRLTWNTIDIVQTLNGTAIESKTGHAFIK
DAMRHHDAVYGGEMSAHHYFREFGYCDSGMIPWLLVIELLSKKTTSLFSLIESRKAKFPVSGELNFTVSDPKTKIQQIKQ
FYADSAIHVTGIDGLSMEFEHWRFNLRSSNTEPLVRLNIETRGDVTLLEQKTAQLTQLLYPK

Sequences:

>Translated_462_residues
MEKLTCFKAYDIRGKLGSELNEEIAYRIGRAYCQWLKPAKVVVGGDARATSVELKNALAKGLMDGGADVIDIGMVGTEEV
YFATRFYGVDGGIQVTASHNPIDYNGMKLVKKDAQPISGDNGLYEIQRLAQDNHFAPPGVRGQLETQLVREHYVEHLLSY
IDLNALLPMKIVVNAGNGAAGPTLDALEAALAKRKVPISFIKINHQPDSSFPNGIPNPMLPENQWSTRQAVCEHQADLGI
AWDGDFDRCFFFDHLGNFIEGYYIVGLLAEKFLLTNNMGKATHNTDKIIHDPRLTWNTIDIVQTLNGTAIESKTGHAFIK
DAMRHHDAVYGGEMSAHHYFREFGYCDSGMIPWLLVIELLSKKTTSLFSLIESRKAKFPVSGELNFTVSDPKTKIQQIKQ
FYADSAIHVTGIDGLSMEFEHWRFNLRSSNTEPLVRLNIETRGDVTLLEQKTAQLTQLLYPK
>Mature_462_residues
MEKLTCFKAYDIRGKLGSELNEEIAYRIGRAYCQWLKPAKVVVGGDARATSVELKNALAKGLMDGGADVIDIGMVGTEEV
YFATRFYGVDGGIQVTASHNPIDYNGMKLVKKDAQPISGDNGLYEIQRLAQDNHFAPPGVRGQLETQLVREHYVEHLLSY
IDLNALLPMKIVVNAGNGAAGPTLDALEAALAKRKVPISFIKINHQPDSSFPNGIPNPMLPENQWSTRQAVCEHQADLGI
AWDGDFDRCFFFDHLGNFIEGYYIVGLLAEKFLLTNNMGKATHNTDKIIHDPRLTWNTIDIVQTLNGTAIESKTGHAFIK
DAMRHHDAVYGGEMSAHHYFREFGYCDSGMIPWLLVIELLSKKTTSLFSLIESRKAKFPVSGELNFTVSDPKTKIQQIKQ
FYADSAIHVTGIDGLSMEFEHWRFNLRSSNTEPLVRLNIETRGDVTLLEQKTAQLTQLLYPK

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen [H]

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family [H]

Homologues:

Organism=Escherichia coli, GI1788361, Length=461, Percent_Identity=60.3036876355748, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI1789566, Length=458, Percent_Identity=24.4541484716157, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841 [H]

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV [H]

EC number: =5.4.2.8 [H]

Molecular weight: Translated: 51593; Mature: 51593

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00710 PGM_PMM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLTCFKAYDIRGKLGSELNEEIAYRIGRAYCQWLKPAKVVVGGDARATSVELKNALAK
CCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
GLMDGGADVIDIGMVGTEEVYFATRFYGVDGGIQVTASHNPIDYNGMKLVKKDAQPISGD
HHHCCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
NGLYEIQRLAQDNHFAPPGVRGQLETQLVREHYVEHLLSYIDLNALLPMKIVVNAGNGAA
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
GPTLDALEAALAKRKVPISFIKINHQPDSSFPNGIPNPMLPENQWSTRQAVCEHQADLGI
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEE
AWDGDFDRCFFFDHLGNFIEGYYIVGLLAEKFLLTNNMGKATHNTDKIIHDPRLTWNTID
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHH
IVQTLNGTAIESKTGHAFIKDAMRHHDAVYGGEMSAHHYFREFGYCDSGMIPWLLVIELL
HHHHCCCCEECCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
SKKTTSLFSLIESRKAKFPVSGELNFTVSDPKTKIQQIKQFYADSAIHVTGIDGLSMEFE
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
HWRFNLRSSNTEPLVRLNIETRGDVTLLEQKTAQLTQLLYPK
HEEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKLTCFKAYDIRGKLGSELNEEIAYRIGRAYCQWLKPAKVVVGGDARATSVELKNALAK
CCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHH
GLMDGGADVIDIGMVGTEEVYFATRFYGVDGGIQVTASHNPIDYNGMKLVKKDAQPISGD
HHHCCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCCCCCCC
NGLYEIQRLAQDNHFAPPGVRGQLETQLVREHYVEHLLSYIDLNALLPMKIVVNAGNGAA
CHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
GPTLDALEAALAKRKVPISFIKINHQPDSSFPNGIPNPMLPENQWSTRQAVCEHQADLGI
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEE
AWDGDFDRCFFFDHLGNFIEGYYIVGLLAEKFLLTNNMGKATHNTDKIIHDPRLTWNTID
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHH
IVQTLNGTAIESKTGHAFIKDAMRHHDAVYGGEMSAHHYFREFGYCDSGMIPWLLVIELL
HHHHCCCCEECCCCCHHHHHHHHHHHCHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
SKKTTSLFSLIESRKAKFPVSGELNFTVSDPKTKIQQIKQFYADSAIHVTGIDGLSMEFE
HHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCEEEECCCCCCEEEE
HWRFNLRSSNTEPLVRLNIETRGDVTLLEQKTAQLTQLLYPK
HEEEEECCCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1383393 [H]