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The map label for this gene is dmlR [H]

Identifier: 170023856

GI number: 170023856

Start: 1786229

End: 1787170

Strand: Reverse

Name: dmlR [H]

Synonym: YPK_1615

Alternate gene names: 170023856

Gene position: 1787170-1786229 (Counterclockwise)

Preceding gene: 170023868

Following gene: 170023855

Centisome position: 38.11

GC content: 52.55

Gene sequence:

>942_bases
ATGAATAATCTGCCGATACTCAGTGACCTACGCGTTTTTCTTCTGGTAGCCCGACGCGCAGGTTTTGCCGCTGCCGCTGA
AGAGATGGGGGTTTCTCCCGCCTACGTCAGTAAGCGTGTCGCGATACTGGAAAAAATGCTGAACGTGGCATTACTGCATC
GCACGACACGGAGAGTCACGATTAGTGAGGATGGCGAGCGGACTTATGAATGGGCACAGCGCATTTTGCACGATGTCGAT
CAGATGATGGACGAGCTGTCAGCCATTCGTCAGGAGCCTCAGGGCACCTTAAGGGTTATCAGCAGTTTTGGTTTTGGCCG
ACGCTTTGTGGCCCCCGCACTCTCTGCGTTAGCTCGCGAGTATCCCCGCCTGGAACTGCGGTTTGATGTGGAGGATCGGC
TAATCGATCTGGTGACTGAAGGGGTTGATTTAGATATCCGCGTAGGCGATAACATTGGCGACAACCTGATTGCTTGCCAA
CTCGCCCCGAATCGCCGCATTCTGTGCGCATCCCCCGATTTTCTACAGCGCTACGGCACACCCAAAGCCCTCAGTGAACT
CCCCGGTTTTGCCTGTTTAGTGATCAAAGAGCGTGACCATCCCTTTGGCATATGGCGCTTGCATAATGGCACCGAAGAAC
AATCGATAAAGGTCACTGGCCCGATGGCCTCTAACCATGGCGAAATCGTTCACCAATGGTGCCTGGACGGGCAAGGTATT
GCTTTACGCTCTTACTGGGATGTTAAAGACAATATTCACTCGGGCAAACTGGTGCATATTCTACCGGAGTACTTTCAATC
GGCTAATATCTGGGCGGTGTACGTCACCCGGCTGGCGATGTCGGCAAAAGTCAGAGTGAGCGTAGAATTTTTACGTCGCT
ATTTTAATGAGCACTACGGCGCAGAAAATACATCGGCAAATGGCGGAACGCTGAAGCGCTGA

Upstream 100 bases:

>100_bases
CAAGTCTCATTTATCAGCGGTAAGAATTGACATCTCCTCCATCCTATAATTGACTAATTTACATACAATACAAAAACTGG
TGATACATAAAACACAGATC

Downstream 100 bases:

>100_bases
TAAACCGCCCGCTTATTGTTCCCAACCCACCGCGTAATCGCCCTAAATAAAGCAAAGTCGGGATATTCAGAGAGAAAGAA
TTAACGTGGAATAAAAACTC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKMLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR

Sequences:

>Translated_313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKMLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR
>Mature_313_residues
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKMLNVALLHRTTRRVTISEDGERTYEWAQRILHDVD
QMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALAREYPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQ
LAPNRRILCASPDFLQRYGTPKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYGAENTSANGGTLKR

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=74.247491638796, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1789440, Length=300, Percent_Identity=49, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=30.6122448979592, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=27.681660899654, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1787589, Length=260, Percent_Identity=26.9230769230769, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.3515358361775, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.3972602739726, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788706, Length=227, Percent_Identity=29.9559471365639, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1786448, Length=258, Percent_Identity=28.6821705426357, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789173, Length=271, Percent_Identity=27.3062730627306, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35376; Mature: 35376

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKMLNVALLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
ISEDGERTYEWAQRILHDVDQMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALARE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
YPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQLAPNRRILCASPDFLQRYGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCHHHHHCCC
PKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
HHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYG
EEEEEECCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AENTSANGGTLKR
CCCCCCCCCCCCC
>Mature Secondary Structure
MNNLPILSDLRVFLLVARRAGFAAAAEEMGVSPAYVSKRVAILEKMLNVALLHRTTRRVT
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEE
ISEDGERTYEWAQRILHDVDQMMDELSAIRQEPQGTLRVISSFGFGRRFVAPALSALARE
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
YPRLELRFDVEDRLIDLVTEGVDLDIRVGDNIGDNLIACQLAPNRRILCASPDFLQRYGT
CCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCHHHHHCCC
PKALSELPGFACLVIKERDHPFGIWRLHNGTEEQSIKVTGPMASNHGEIVHQWCLDGQGI
HHHHHHCCCEEEEEEEECCCCEEEEEECCCCCCCEEEEECCCCCCCCHHHHHHHCCCCCE
ALRSYWDVKDNIHSGKLVHILPEYFQSANIWAVYVTRLAMSAKVRVSVEFLRRYFNEHYG
EEEEEECCCCCCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
AENTSANGGTLKR
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]