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The map label for this gene is rbsR [H]

Identifier: 170023855

GI number: 170023855

Start: 1784854

End: 1785858

Strand: Reverse

Name: rbsR [H]

Synonym: YPK_1614

Alternate gene names: 170023855

Gene position: 1785858-1784854 (Counterclockwise)

Preceding gene: 170023856

Following gene: 170023846

Centisome position: 38.08

GC content: 40.6

Gene sequence:

>1005_bases
GTGTCGAAAGCTTATTCAAATGCCACGATTGTGGATATTGCCCGTCGAGCTAATGTGACAAACATCACGGTTTCCCGTAC
TTTTAATCAACCTGGATTAGTTAAACAAGAAACCCGTGAAAAAATTCATGCTATCGCAAAAGAACTTAATTACGTCCCCA
ATGCTTTTGCTCAGGGATTAAAACGCAGTAGCAGCCAAATTATTGGTATTGTCACCAGCAGTATGTATAACCCTTTTTAC
TCTGAATTAATTCAAACGGTCTCCAGAATTGCACGAACACAGTGTTATCAAATTATGCTGTTTGATACCGATGGCAGTGA
AGAGGCAGAAATGGAGGCTATTCAGGCGCTATTTGGCTATAAAGCCTGTGGAATTCTTTTGTCACCGGTTCGCGATGATA
AAAACTACCAACCGGCCTATCTCGATTTAGCCGAAACCTATCGCGTTCCATTGATTCTTATTGATAGAGATATCTATAAT
CGGCAACTGAGCGGAGTATTTCTTAATAACAAAGAGATCGGGTTACTGGCGGGGAAATACCTGTCTGAGCAGCCAGAGAG
GAAAATGCTAATCATTGGTGGGCCCGCAGAATCAGAGATAACACGGGTACGTATTGAAGGGATTATCAATGCGTTAGAAA
ATAAAAAAAATGACGTGCATATTATTAATGGTGATTACGATTTTATTTCACAGGAAGCAGCCGTTAGAGATTATCTCTCT
TCCCCTCAGAATCAACCTGATTATATTATTGGCTTAAATGGAATTTTGACACTTGGCGCTATTGCAATTTGTCATGAACT
GAAAATTTATAATAATATAAAATTCTTCTCGATTGATGAGCCACCTAAAGCAGCAGATTATGGCCTTCATATTGCCGGTG
TTTACCACGATACCCAGATGCTGGGGGAGATTGCTGCAGAACTCCTTTTTAATGCCATTAAAAAACCCCACAACGATCAG
GCAGTAAGACGCGAATTCTTCACCGGATCTCTGCTCACCCACTAA

Upstream 100 bases:

>100_bases
GCCTACCTGCCTCTTGAAGTAACTTGGGTATATATCTGGCATACTTTAAGCGGCTTCCCTACAATAAGCATTCAGTTGAC
GAATAAAGCAAGGAATAAAG

Downstream 100 bases:

>100_bases
AACGGCCTACGAAAATATGGGGCCCCCATGTTCAACCCGCCGTAATGGCGGCCATTATCGCGCCGCCATTACCCTCACTG
CATCAACTTAATTACTGTAT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFY
SELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYN
RQLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
SPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQ
AVRREFFTGSLLTH

Sequences:

>Translated_334_residues
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFY
SELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYN
RQLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
SPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQ
AVRREFFTGSLLTH
>Mature_333_residues
SKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGLKRSSSQIIGIVTSSMYNPFYS
ELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGYKACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNR
QLSGVFLNNKEIGLLAGKYLSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLSS
PQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQMLGEIAAELLFNAIKKPHNDQA
VRREFFTGSLLTH

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=27.7070063694268, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=27.1565495207668, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790369, Length=294, Percent_Identity=25.5102040816327, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1789068, Length=266, Percent_Identity=26.3157894736842, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786268, Length=216, Percent_Identity=26.3888888888889, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1786540, Length=312, Percent_Identity=25, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1788474, Length=240, Percent_Identity=28.3333333333333, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994940, Length=141, Percent_Identity=28.3687943262411, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789202, Length=242, Percent_Identity=24.3801652892562, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790715, Length=330, Percent_Identity=23.9393939393939, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=25.5255255255255, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787906, Length=199, Percent_Identity=23.6180904522613, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37484; Mature: 37353

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGL
CCCCCCCCHHEEHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSSSQIIGIVTSSMYNPFYSELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGY
HCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHH
KACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNRQLSGVFLNNKEIGLLAGKY
HHHHHEEECCCCCCCCCCHHEEHHHHCCCCEEEEECHHHCCEECEEEECCCCEEEEECHH
LSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
SPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQM
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
LGEIAAELLFNAIKKPHNDQAVRREFFTGSLLTH
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
SKAYSNATIVDIARRANVTNITVSRTFNQPGLVKQETREKIHAIAKELNYVPNAFAQGL
CCCCCCCHHEEHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KRSSSQIIGIVTSSMYNPFYSELIQTVSRIARTQCYQIMLFDTDGSEEAEMEAIQALFGY
HCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHH
KACGILLSPVRDDKNYQPAYLDLAETYRVPLILIDRDIYNRQLSGVFLNNKEIGLLAGKY
HHHHHEEECCCCCCCCCCHHEEHHHHCCCCEEEEECHHHCCEECEEEECCCCEEEEECHH
LSEQPERKMLIIGGPAESEITRVRIEGIINALENKKNDVHIINGDYDFISQEAAVRDYLS
HCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHH
SPQNQPDYIIGLNGILTLGAIAICHELKIYNNIKFFSIDEPPKAADYGLHIAGVYHDTQM
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEEEHHHHH
LGEIAAELLFNAIKKPHNDQAVRREFFTGSLLTH
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]