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The map label for this gene is mepA

Identifier: 170023756

GI number: 170023756

Start: 1676943

End: 1677749

Strand: Direct

Name: mepA

Synonym: YPK_1515

Alternate gene names: 170023756

Gene position: 1676943-1677749 (Clockwise)

Preceding gene: 170023755

Following gene: 170023757

Centisome position: 35.76

GC content: 53.53

Gene sequence:

>807_bases
ATGGTGGCGTTGGTCACCATGGTCCCTGTCATGGCGGCCACGCCATGGCAAAAGATTACGCACCCGGTGGCGGGTTCTCC
ACAGTCAATCGGTGGCTTTGCTAATGGTTGCGTGATTGGCGCGCAGCCTTTGCCGCTCGAGTCTGCAGACTATCAAGTGA
TGCGTTCGGATCAGCGCCGTTATTTTGGTCACCCTGATTTACTGAATTTTATTCACCGTCTCAGTGCACAAGCTCATCAA
CAACAATTGGGCACGGTGTTGATTGGTGATATGGCGATGCCTGCAGGCGGGCGTTTTAGCAGTGGGCATGCCAGCCACCA
GTCTGGTTTGGATGTGGATATTTGGCTACAACTGCCAAAGCAGCGTTGGAGCCAGCAGCAGTTATTGAAACCGCAGCCGA
TAGACTTGGTCGCGGTTGATGGCAAGCGGGTGATCCCCGCACTTTGGCAGCCACAAATTGAGAGTTTGATCAAATTGGCC
GCCAAAGATAACGATGTCACGCGTATCTTCGTCAATCCGGCAATTAAAAAGCAGCTTTGTCTGGATGCAGGTGCCGATCG
TCAATGGCTGCATAAAGTCCGGCCGTGGTTTGCTCATCGTGCACACATGCATGTGCGCTTACGTTGTCCGGCCAATAGCC
TAGAGTGTGTGGATCAAGACACTCCACCGCCGGGCGATGGCTGTGGTGCTGAGCTGGAAAGCTGGTTCCAACCGCCTCCG
CCAAGTGCTAAACCAGGTAAAACCTTGCCACCGCCATTACCGCCTTCTTGTCAGGCTTTACTGGATAACCACTTCGCTAC
GGAATAA

Upstream 100 bases:

>100_bases
TCGTACTTATGGATCACCTACTGCGCCAGCGCGCGCAGTGCGGCGATGTGGCTTCAGACGTTCCGCGCTGGTAGATAGCA
TGAAAAATTGGATAGTGGGT

Downstream 100 bases:

>100_bases
GTCATGGATTGGTTTACCCTTGGTCCTGCCGTTTTGGGGATACTCTTTGCCGTTGCATTATTAGCCGGGTTCATTGATTC
CATTGCCGGCGGGGGCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQ
QQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLA
AKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPP
PSAKPGKTLPPPLPPSCQALLDNHFATE

Sequences:

>Translated_268_residues
MVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQ
QQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLA
AKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPP
PSAKPGKTLPPPLPPSCQALLDNHFATE
>Mature_268_residues
MVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHRLSAQAHQ
QQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLA
AKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPP
PSAKPGKTLPPPLPPSCQALLDNHFATE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=73.4126984126984, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEPA_YERPA (Q1C665)

Other databases:

- EMBL:   CP000308
- RefSeq:   YP_652002.1
- ProteinModelPortal:   Q1C665
- SMR:   Q1C665
- STRING:   Q1C665
- GeneID:   4122003
- GenomeReviews:   CP000308_GR
- KEGG:   ypa:YPA_2091
- eggNOG:   COG3770
- HOGENOM:   HBG296858
- ProtClustDB:   PRK09429
- BioCyc:   YPES360102:YPA_2091-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01623
- InterPro:   IPR009045
- InterPro:   IPR005073
- Gene3D:   G3DSA:3.30.1380.10
- PIRSF:   PIRSF018455

Pfam domain/function: PF03411 Peptidase_M74; SSF55166 Hedgehog_sig_N

EC number: 3.4.99.- [C]

Molecular weight: Translated: 29464; Mature: 29464

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRR
CEEHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
YFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPK
HCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCH
QRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLC
HHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHH
LDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPP
HCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCC
PSAKPGKTLPPPLPPSCQALLDNHFATE
CCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRR
CEEHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHHHHHHHHH
YFGHPDLLNFIHRLSAQAHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPK
HCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCH
QRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNPAIKKQLC
HHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHH
LDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELESWFQPPP
HCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHHHHCCCCC
PSAKPGKTLPPPLPPSCQALLDNHFATE
CCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA