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The map label for this gene is yegS [C]

Identifier: 170023566

GI number: 170023566

Start: 1453270

End: 1454160

Strand: Reverse

Name: yegS [C]

Synonym: YPK_1324

Alternate gene names: 170023566

Gene position: 1454160-1453270 (Counterclockwise)

Preceding gene: 170023567

Following gene: 170023564

Centisome position: 31.01

GC content: 52.08

Gene sequence:

>891_bases
ATGCCACACACTCTTTTGATCCTCAACGGCAAAGAATCAGGCAACCCTGAGGTCCGCGAAGCGGTCAAAAACGTACGCGA
CGAAGGCTTAACGCTTCATGTCCGTATCACCTGGGAGCACGGTGATGCAAAACGCTATGTTGAAGAGGCCGCCACCTTGG
CTGTCAGTACCGTTATCGCTGGAGGCGGCGATGGCACCATTAATGAAGTGGCAACAGCATTGATGAGTTTACCTGCGGAT
AAACGCCCTTGTCTGGGGATCCTACCGCTGGGAACCGCCAATGATTTTGCGACTGGCTGCAATATCCCTTTGCAAATAGA
GAACGCACTGCAACTGGCGGTCAAAGGCCGGGCGGTGGCAATCGATCTGGCTCAAGTCAACGGTGAGCATTACTTTATCA
ATATGGCAACCGGGGGTTTTGGTACCCGAATTACCACCGAAACACCCGATAAACTCAAAGCGGCCTTGGGCGGAGTCTCT
TACTTTATTCATGGGCTGATGCGCCTTGATGCCCTAAAAGCAGACAGTTGCAAAATCCATGGCCCTGACTTTCATTGGTC
GGGGGATGCGCTGGTCATTGGCATCGGCAACGGTAAACAGGCCGGGGGCGGACAACTTCTTTGCCCCGATGCACTGATTA
ATGACGGCCTGATGCAATTACGCCTACTGACAGCAAAGGAGTTACTCCCTGCAGTGCTGAGTACCCTGTTCAATGGTGAG
AAAAATAAGAATGTTATCGACGCCACGGTACCTTGGCTCGATATCACTGCACCGAACGATATCACCTTCAATCTGGATGG
TGAGCCACTGTCCGGCAGACATTTCCACATTGAAATCTTACCCCATGCGATTCAGTGCCGGCTACCACCGAATTGCCCGT
TGTTAGGCTAA

Upstream 100 bases:

>100_bases
CGAGTGCAGCTAATTTTTCACCGATACAAGCCTCATCGATACAAGTACTGCTGCAGCACCAAGAATGAAGGGGCTCCCAT
ATAAATGATTTGGAGCACAT

Downstream 100 bases:

>100_bases
ACGCCACTTAGCCCTACACACTCAGTGCCATCTGGAGGACGGCGGGCGACGAGTGTAGAACACGATAAATGAATGATAAA
ACGTGTGAAATGCGGCAGAA

Product: lipid kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MPHTLLILNGKESGNPEVREAVKNVRDEGLTLHVRITWEHGDAKRYVEEAATLAVSTVIAGGGDGTINEVATALMSLPAD
KRPCLGILPLGTANDFATGCNIPLQIENALQLAVKGRAVAIDLAQVNGEHYFINMATGGFGTRITTETPDKLKAALGGVS
YFIHGLMRLDALKADSCKIHGPDFHWSGDALVIGIGNGKQAGGGQLLCPDALINDGLMQLRLLTAKELLPAVLSTLFNGE
KNKNVIDATVPWLDITAPNDITFNLDGEPLSGRHFHIEILPHAIQCRLPPNCPLLG

Sequences:

>Translated_296_residues
MPHTLLILNGKESGNPEVREAVKNVRDEGLTLHVRITWEHGDAKRYVEEAATLAVSTVIAGGGDGTINEVATALMSLPAD
KRPCLGILPLGTANDFATGCNIPLQIENALQLAVKGRAVAIDLAQVNGEHYFINMATGGFGTRITTETPDKLKAALGGVS
YFIHGLMRLDALKADSCKIHGPDFHWSGDALVIGIGNGKQAGGGQLLCPDALINDGLMQLRLLTAKELLPAVLSTLFNGE
KNKNVIDATVPWLDITAPNDITFNLDGEPLSGRHFHIEILPHAIQCRLPPNCPLLG
>Mature_295_residues
PHTLLILNGKESGNPEVREAVKNVRDEGLTLHVRITWEHGDAKRYVEEAATLAVSTVIAGGGDGTINEVATALMSLPADK
RPCLGILPLGTANDFATGCNIPLQIENALQLAVKGRAVAIDLAQVNGEHYFINMATGGFGTRITTETPDKLKAALGGVSY
FIHGLMRLDALKADSCKIHGPDFHWSGDALVIGIGNGKQAGGGQLLCPDALINDGLMQLRLLTAKELLPAVLSTLFNGEK
NKNVIDATVPWLDITAPNDITFNLDGEPLSGRHFHIEILPHAIQCRLPPNCPLLG

Specific function: Probably phosphorylates lipids; the in vivo substrate is unknown

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain

Homologues:

Organism=Escherichia coli, GI1788402, Length=295, Percent_Identity=67.7966101694915, Blast_Score=419, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YEGS_YERP3 (A7FG10)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400188.1
- ProteinModelPortal:   A7FG10
- SMR:   A7FG10
- STRING:   A7FG10
- GeneID:   5384373
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1207
- eggNOG:   COG1597
- HOGENOM:   HBG698136
- OMA:   FRIECVP
- ProtClustDB:   PRK13054
- BioCyc:   YPSE349747:YPSIP31758_1207-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01377
- InterPro:   IPR005218
- InterPro:   IPR001206
- InterPro:   IPR022433
- InterPro:   IPR022862
- SMART:   SM00046
- TIGRFAMs:   TIGR00147
- TIGRFAMs:   TIGR03702

Pfam domain/function: PF00781 DAGK_cat

EC number: NA

Molecular weight: Translated: 31536; Mature: 31404

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50146 DAGK

Important sites: ACT_SITE 268-268 BINDING 37-37 BINDING 92-92

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHTLLILNGKESGNPEVREAVKNVRDEGLTLHVRITWEHGDAKRYVEEAATLAVSTVIA
CCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHHEEEEEEE
GGGDGTINEVATALMSLPADKRPCLGILPLGTANDFATGCNIPLQIENALQLAVKGRAVA
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCEEE
IDLAQVNGEHYFINMATGGFGTRITTETPDKLKAALGGVSYFIHGLMRLDALKADSCKIH
EEEEEECCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
GPDFHWSGDALVIGIGNGKQAGGGQLLCPDALINDGLMQLRLLTAKELLPAVLSTLFNGE
CCCCEECCCEEEEECCCCCCCCCCCEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
KNKNVIDATVPWLDITAPNDITFNLDGEPLSGRHFHIEILPHAIQCRLPPNCPLLG
CCCEEEEEECCEEEEECCCCEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCCCC
>Mature Secondary Structure 
PHTLLILNGKESGNPEVREAVKNVRDEGLTLHVRITWEHGDAKRYVEEAATLAVSTVIA
CCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEEEECCCHHHHHHHHHHHEEEEEEE
GGGDGTINEVATALMSLPADKRPCLGILPLGTANDFATGCNIPLQIENALQLAVKGRAVA
CCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCEEE
IDLAQVNGEHYFINMATGGFGTRITTETPDKLKAALGGVSYFIHGLMRLDALKADSCKIH
EEEEEECCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
GPDFHWSGDALVIGIGNGKQAGGGQLLCPDALINDGLMQLRLLTAKELLPAVLSTLFNGE
CCCCEECCCEEEEECCCCCCCCCCCEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
KNKNVIDATVPWLDITAPNDITFNLDGEPLSGRHFHIEILPHAIQCRLPPNCPLLG
CCCEEEEEECCEEEEECCCCEEEEECCCCCCCCEEEEEEECCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA