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The map label for this gene is cysJ [H]

Identifier: 169629571

GI number: 169629571

Start: 2535963

End: 2537705

Strand: Reverse

Name: cysJ [H]

Synonym: MAB_2485c

Alternate gene names: 169629571

Gene position: 2537705-2535963 (Counterclockwise)

Preceding gene: 169629572

Following gene: 169629570

Centisome position: 50.08

GC content: 64.49

Gene sequence:

>1743_bases
ATGAAGATCGCCTACATTCCCGAAGACGCACCCTTCAACGAGGACCAGAGAGCATGGCTTTCGGGCTTCCTCGCCGGGTT
GCACTCCCGCCTCGCCATGAACCTGGCGGCGCAACCGCCGGCGGCCACCGCGGATCTGGCTGCTCCCCGGCCACCACTGC
GCATCCTGTACGGCACCCAGACGGGCAACGCGGAAATCGTGGCGGGCGACGCCGCGGCGGCCGCCAAAGCACAAGGTTTC
GACGTGACCGTCAGCGGCCTCGACGAAATCGCCCTCGACGAGTTCGCGGGCCTGCGATACGTCCTGATCATCACCTCCAC
CTACGGCGAGGGCGAGATGCCCGACAATGCCGAATTATTCTGGGATGCACTGTCTTCGGCGGTCGCTCCACGACTCGAGG
GAGTGTCCTACGGCGTGCTGGCCCTGGGTGACACCAGCTACGACGGGTTCTGCCAGGCCGGCAAGCTGATCGATACCCGT
CTTGAGCAACTGGGCGCGAGCCGCGTGGTCGGGCGCACCGACTGCGACGTCGATTACGAGGCCCAGGCCGCGGAGTGGGT
TCAGGGCGCAGTGGCAAGCCTGGTCACTCTGGCCGGCGCCAGCGGCAGTCCGGGCACTCCCCCTCCGAGCACCGTCGCGC
GTTCCGGGTGGACCCGCAAGAACCCGTTCCCCGCCGAGGTCCCGGTCAACCGACTGCTCTCACGGGCAGGCTCTGACAAG
GAGATTCGCCACTACGAGTTCGCACTCGCCGACAGCGGGATCACCTACGAAGCGGGCGACGCACTCGCGGTGATTCCGGA
GAACGACCCGGCGCTGGTCGAGGCGATCGCCGATCACTTCCGGGTCTCGACCGGCACACTCGTCGATGGGGAGCCGCTCG
GAGAACTGCTACGCCATCGGTACGAGATCAGTACTCCGTCGCGAGACCTGTTGAGCGAGGTGGAAAGCCGTGCCGAAAGC
GAGGAGTTCTCGCACGCGCTGCGCGGCGGGGTCAAAGAGGTACTGGATACCTGGCTATATGGCAAGGACATTCTCGACAT
CCTGCGTATCGGCGGCGACGCTTTGAGCCTCGAAGAGTTCATGGGACTGCTCAAGCCCCTACAGCATCGCGCCTACTCGA
TCTCATCGAGCGCGCTGGCTCACCCGGATAGGATCCACCTGACCGTGGCCAGCGTCCGGTATCAGAGCCTGGGCCGTGAG
CGCGGCGGCGTGTGCTCGACGTTCTTGGCAGACCGCGGCAGTAACGCACGGATCTTCCTGCAACCCAACAAATCATTCCG
AGTGCCCAGCGACGACGAAGCCCCGATGATCATGGTGGGCCCCGGCACGGGTGTGGCCCCATTCCGCGCATTCCTGCAGG
AACGCGAGCAGCGGGGAGCGAGGGGCAACAACTGGCTGTTCTTCGGCGACCAGCACCGGGCACATGACTACATCTACGAG
AACGAGATCGACACCTGGCACTCTTCTGGCCTCCTGGATCGCCTCGATCTCGCCTTCTCCCGCGATCAAGCCGAGAAGGT
CTATGTGCAGAACCGAATGCGCGAGCATGGCAGCGATCTGTTCGCATGGCTGCAGGACGGCGGCCACTTCTACGTCTGCG
GTGACGCGACACGCATGGCCAAGGACGTGGACAAGGCGCTGCACGAGATCGTCGGCGAGCACGGCGGATTCAGCGACGAT
GAGGCCTCCGAATATGTGAATACCCTCAAGCGGGAAAAGCGGTACGTCCGCGATGTGTACTGA

Upstream 100 bases:

>100_bases
AAGATCGGCCGGCTGCCCTCTTTCGAAGAGCTGTTCGCGCATCGCGCACTGCCTGCCGTTGCCCTCTCCGGACCGTCCCT
CCAGACCACGTGAAGAAGCC

Downstream 100 bases:

>100_bases
CGGCCGACGCTGCGAACACTGCCCTGCCCGACGGATCGTCGCGGGGCCCAAAGCCATTGCCGAAGCACTTCCCGCGCTCG
GCACGGTGGTCGCCGTCACC

Product: sulfite reductase

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MKIAYIPEDAPFNEDQRAWLSGFLAGLHSRLAMNLAAQPPAATADLAAPRPPLRILYGTQTGNAEIVAGDAAAAAKAQGF
DVTVSGLDEIALDEFAGLRYVLIITSTYGEGEMPDNAELFWDALSSAVAPRLEGVSYGVLALGDTSYDGFCQAGKLIDTR
LEQLGASRVVGRTDCDVDYEAQAAEWVQGAVASLVTLAGASGSPGTPPPSTVARSGWTRKNPFPAEVPVNRLLSRAGSDK
EIRHYEFALADSGITYEAGDALAVIPENDPALVEAIADHFRVSTGTLVDGEPLGELLRHRYEISTPSRDLLSEVESRAES
EEFSHALRGGVKEVLDTWLYGKDILDILRIGGDALSLEEFMGLLKPLQHRAYSISSSALAHPDRIHLTVASVRYQSLGRE
RGGVCSTFLADRGSNARIFLQPNKSFRVPSDDEAPMIMVGPGTGVAPFRAFLQEREQRGARGNNWLFFGDQHRAHDYIYE
NEIDTWHSSGLLDRLDLAFSRDQAEKVYVQNRMREHGSDLFAWLQDGGHFYVCGDATRMAKDVDKALHEIVGEHGGFSDD
EASEYVNTLKREKRYVRDVY

Sequences:

>Translated_580_residues
MKIAYIPEDAPFNEDQRAWLSGFLAGLHSRLAMNLAAQPPAATADLAAPRPPLRILYGTQTGNAEIVAGDAAAAAKAQGF
DVTVSGLDEIALDEFAGLRYVLIITSTYGEGEMPDNAELFWDALSSAVAPRLEGVSYGVLALGDTSYDGFCQAGKLIDTR
LEQLGASRVVGRTDCDVDYEAQAAEWVQGAVASLVTLAGASGSPGTPPPSTVARSGWTRKNPFPAEVPVNRLLSRAGSDK
EIRHYEFALADSGITYEAGDALAVIPENDPALVEAIADHFRVSTGTLVDGEPLGELLRHRYEISTPSRDLLSEVESRAES
EEFSHALRGGVKEVLDTWLYGKDILDILRIGGDALSLEEFMGLLKPLQHRAYSISSSALAHPDRIHLTVASVRYQSLGRE
RGGVCSTFLADRGSNARIFLQPNKSFRVPSDDEAPMIMVGPGTGVAPFRAFLQEREQRGARGNNWLFFGDQHRAHDYIYE
NEIDTWHSSGLLDRLDLAFSRDQAEKVYVQNRMREHGSDLFAWLQDGGHFYVCGDATRMAKDVDKALHEIVGEHGGFSDD
EASEYVNTLKREKRYVRDVY
>Mature_580_residues
MKIAYIPEDAPFNEDQRAWLSGFLAGLHSRLAMNLAAQPPAATADLAAPRPPLRILYGTQTGNAEIVAGDAAAAAKAQGF
DVTVSGLDEIALDEFAGLRYVLIITSTYGEGEMPDNAELFWDALSSAVAPRLEGVSYGVLALGDTSYDGFCQAGKLIDTR
LEQLGASRVVGRTDCDVDYEAQAAEWVQGAVASLVTLAGASGSPGTPPPSTVARSGWTRKNPFPAEVPVNRLLSRAGSDK
EIRHYEFALADSGITYEAGDALAVIPENDPALVEAIADHFRVSTGTLVDGEPLGELLRHRYEISTPSRDLLSEVESRAES
EEFSHALRGGVKEVLDTWLYGKDILDILRIGGDALSLEEFMGLLKPLQHRAYSISSSALAHPDRIHLTVASVRYQSLGRE
RGGVCSTFLADRGSNARIFLQPNKSFRVPSDDEAPMIMVGPGTGVAPFRAFLQEREQRGARGNNWLFFGDQHRAHDYIYE
NEIDTWHSSGLLDRLDLAFSRDQAEKVYVQNRMREHGSDLFAWLQDGGHFYVCGDATRMAKDVDKALHEIVGEHGGFSDD
EASEYVNTLKREKRYVRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI127139033, Length=600, Percent_Identity=32, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI7657393, Length=605, Percent_Identity=30.7438016528926, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI221316705, Length=614, Percent_Identity=30.2931596091205, Blast_Score=213, Evalue=4e-55,
Organism=Homo sapiens, GI221316709, Length=605, Percent_Identity=30.2479338842975, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI221316707, Length=536, Percent_Identity=30.9701492537313, Blast_Score=189, Evalue=6e-48,
Organism=Homo sapiens, GI24041029, Length=602, Percent_Identity=29.2358803986711, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI10835173, Length=660, Percent_Identity=28.4848484848485, Blast_Score=179, Evalue=5e-45,
Organism=Homo sapiens, GI40254422, Length=641, Percent_Identity=27.4570982839314, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI169790956, Length=256, Percent_Identity=38.28125, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI169790958, Length=256, Percent_Identity=38.28125, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1789123, Length=575, Percent_Identity=39.4782608695652, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1789154, Length=121, Percent_Identity=37.1900826446281, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554134, Length=594, Percent_Identity=31.4814814814815, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17566446, Length=573, Percent_Identity=28.0977312390925, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17531441, Length=269, Percent_Identity=27.1375464684015, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6321832, Length=635, Percent_Identity=28.503937007874, Blast_Score=173, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6321143, Length=391, Percent_Identity=29.923273657289, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325305, Length=629, Percent_Identity=24.0063593004769, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322597, Length=184, Percent_Identity=31.5217391304348, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137192, Length=600, Percent_Identity=32.3333333333333, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI24582192, Length=522, Percent_Identity=32.7586206896552, Blast_Score=228, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24660907, Length=594, Percent_Identity=28.4511784511784, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24660903, Length=594, Percent_Identity=28.4511784511784, Blast_Score=181, Evalue=9e-46,
Organism=Drosophila melanogaster, GI24583543, Length=382, Percent_Identity=31.9371727748691, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI78706876, Length=382, Percent_Identity=31.9371727748691, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI78706872, Length=382, Percent_Identity=31.9371727748691, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24647438, Length=415, Percent_Identity=23.6144578313253, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 63398; Mature: 63398

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAYIPEDAPFNEDQRAWLSGFLAGLHSRLAMNLAAQPPAATADLAAPRPPLRILYGTQ
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
TGNAEIVAGDAAAAAKAQGFDVTVSGLDEIALDEFAGLRYVLIITSTYGEGEMPDNAELF
CCCCEEEECCHHHHHHCCCCEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHH
WDALSSAVAPRLEGVSYGVLALGDTSYDGFCQAGKLIDTRLEQLGASRVVGRTDCDVDYE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHCHHHCCCCCCCCCCCC
AQAAEWVQGAVASLVTLAGASGSPGTPPPSTVARSGWTRKNPFPAEVPVNRLLSRAGSDK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
EIRHYEFALADSGITYEAGDALAVIPENDPALVEAIADHFRVSTGTLVDGEPLGELLRHR
CHHEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHH
YEISTPSRDLLSEVESRAESEEFSHALRGGVKEVLDTWLYGKDILDILRIGGDALSLEEF
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MGLLKPLQHRAYSISSSALAHPDRIHLTVASVRYQSLGRERGGVCSTFLADRGSNARIFL
HHHHHHHHHHHHCCCCHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEE
QPNKSFRVPSDDEAPMIMVGPGTGVAPFRAFLQEREQRGARGNNWLFFGDQHRAHDYIYE
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEC
NEIDTWHSSGLLDRLDLAFSRDQAEKVYVQNRMREHGSDLFAWLQDGGHFYVCGDATRMA
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
KDVDKALHEIVGEHGGFSDDEASEYVNTLKREKRYVRDVY
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIAYIPEDAPFNEDQRAWLSGFLAGLHSRLAMNLAAQPPAATADLAAPRPPLRILYGTQ
CEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECC
TGNAEIVAGDAAAAAKAQGFDVTVSGLDEIALDEFAGLRYVLIITSTYGEGEMPDNAELF
CCCCEEEECCHHHHHHCCCCEEEECCCHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHH
WDALSSAVAPRLEGVSYGVLALGDTSYDGFCQAGKLIDTRLEQLGASRVVGRTDCDVDYE
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHCCHHHHHHHHHHCHHHCCCCCCCCCCCC
AQAAEWVQGAVASLVTLAGASGSPGTPPPSTVARSGWTRKNPFPAEVPVNRLLSRAGSDK
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
EIRHYEFALADSGITYEAGDALAVIPENDPALVEAIADHFRVSTGTLVDGEPLGELLRHR
CHHEEEEEEECCCCEEECCCEEEEECCCCHHHHHHHHHHHCCCCCCEECCHHHHHHHHHH
YEISTPSRDLLSEVESRAESEEFSHALRGGVKEVLDTWLYGKDILDILRIGGDALSLEEF
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MGLLKPLQHRAYSISSSALAHPDRIHLTVASVRYQSLGRERGGVCSTFLADRGSNARIFL
HHHHHHHHHHHHCCCCHHCCCCCEEEEEHHHHHHHHHCCCCCCCHHHHHHCCCCCCEEEE
QPNKSFRVPSDDEAPMIMVGPGTGVAPFRAFLQEREQRGARGNNWLFFGDQHRAHDYIYE
CCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEC
NEIDTWHSSGLLDRLDLAFSRDQAEKVYVQNRMREHGSDLFAWLQDGGHFYVCGDATRMA
CCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHH
KDVDKALHEIVGEHGGFSDDEASEYVNTLKREKRYVRDVY
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]