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The map label for this gene is gatB

Identifier: 169824269

GI number: 169824269

Start: 635989

End: 637410

Strand: Direct

Name: gatB

Synonym: FMG_0572

Alternate gene names: 169824269

Gene position: 635989-637410 (Clockwise)

Preceding gene: 169824268

Following gene: 169824270

Centisome position: 35.38

GC content: 30.8

Gene sequence:

>1422_bases
ATGAACTTAAAAACACTTATAGGATTGGAAATTCACGTAGAACTATCAACTAAAACTAAAATGTTTTGTGGTTGTAAGAA
TGAGTTTGGACAAATCCCAAATACAAATGTCTGCCCAATTTGTTTAGGTCATCCAGGAGCACTTCCTCACATGAACAAAC
AAGCTTTAAGATATGCTATAATGGCTGGACTTGCTTTTGATTGTGACATTGCTAATGAATTTAAGATGGACAGGAAAAAA
TACTTCTATCCAGATTTAGTTAAAGGATATCAAATCACACAAGAAGATCAACCACTTTGTACAAATGGATATATCGAATT
AAAAACCTCAACTAAAAATAAAAAAGTTAGAATTAGAAGAATCCACATAGAAGAAGATACTGGTAAATCAATCCACAACG
AAAGTGGAAATACTCTAATGGATTATAACAGGGCGGGAGTTCCACTAATTGAAATAGTTAGTGAACCAGACATGAGCAAT
CCTGATGAAGCGAGAGAATTCTTAGAAACTTTAAGAGAAAGAATTAAATATTTGGAAATATCAGATGTTAAGATGAGTGA
AGGATCATTAAGATGTGATGTTAACATCAATGTTTACGATGAAGATTCTGATTTTAAAACTAAAATTTCTGAAATCAAAA
ACTTAAACTCATTCAAATCAGTTTCAAAAGCTTTAATTTACGAACAAGAAAGACATATGCAACTAGCTAAGGAAAACAAA
ATTGGCGAAAAAGAAACTAGAAGATGGGATGAAGATACTCAAACTACAATAGTTATGAGACACAAAGAAGAAGGAAATGA
TTATAGATTTTCAGTTGAAGGCGATATTCCTAAAACTTATGTAGAACAATCTTATATCGAAGAAATCAAAAAGAATTTAC
CAGAACTTCCAGAAATGCGTAAAGAAAGATTCATGAACGAATACAAGATTGATGAATACGACGCTGATATTTTAACAAGA
AATGGATACTTAGCAGACTACTACGAAAAAGTTGTTGAAATTTCAAATGATCCTGTTCAATCTTCAAATTGGTTATTGGG
AGATGTTCTTCGTCAAGTAAACGAAAATGAAATTGAAATTGAAGAAATGAATATGTCTGCTGAAAATTTGGCAAAATTGA
TTAAACTTTCTTCAGCTAAAAAGATAAATAATCAAACTGCAAAGAAAGTTTTGAGAGAAATGTTCAACGAAAACTTTGAT
CCAGAAGTTTACGTCAAAGAAAAAGGATTATTACAAGTTGATGACGATAATCTTTTACAACAAATTGTAGATGAAGTTGT
AGCTGAAAATCCAGAATCTATCGAAAGTATCAGAAATGGTAAAGATCGTGCAATAGGATTCTTAGTTGGACAATGCATGA
AAAAGTCAAAAGGTAAAGGAAATCCACAAAAATTCAACGAATTAATAAAAGAGAAAATCTAA

Upstream 100 bases:

>100_bases
CCTTACAAATCAGGAATATCTACTAGTTTGCAACTAATTGCAAATAGTCACGACGAAGAAAATTTATTGAATGCAGCTTT
AGCGTTTGAAAGGAGATAGC

Downstream 100 bases:

>100_bases
TGAAGAAAATATATTTATCTACAGGAAACAAAGGAAAAGTTTCTGAAATAAAAGAAATACTTTCTGATTTAACATATGAT
GTGTATTCTAAATCTGAATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNLKTLIGLEIHVELSTKTKMFCGCKNEFGQIPNTNVCPICLGHPGALPHMNKQALRYAIMAGLAFDCDIANEFKMDRKK
YFYPDLVKGYQITQEDQPLCTNGYIELKTSTKNKKVRIRRIHIEEDTGKSIHNESGNTLMDYNRAGVPLIEIVSEPDMSN
PDEAREFLETLRERIKYLEISDVKMSEGSLRCDVNINVYDEDSDFKTKISEIKNLNSFKSVSKALIYEQERHMQLAKENK
IGEKETRRWDEDTQTTIVMRHKEEGNDYRFSVEGDIPKTYVEQSYIEEIKKNLPELPEMRKERFMNEYKIDEYDADILTR
NGYLADYYEKVVEISNDPVQSSNWLLGDVLRQVNENEIEIEEMNMSAENLAKLIKLSSAKKINNQTAKKVLREMFNENFD
PEVYVKEKGLLQVDDDNLLQQIVDEVVAENPESIESIRNGKDRAIGFLVGQCMKKSKGKGNPQKFNELIKEKI

Sequences:

>Translated_473_residues
MNLKTLIGLEIHVELSTKTKMFCGCKNEFGQIPNTNVCPICLGHPGALPHMNKQALRYAIMAGLAFDCDIANEFKMDRKK
YFYPDLVKGYQITQEDQPLCTNGYIELKTSTKNKKVRIRRIHIEEDTGKSIHNESGNTLMDYNRAGVPLIEIVSEPDMSN
PDEAREFLETLRERIKYLEISDVKMSEGSLRCDVNINVYDEDSDFKTKISEIKNLNSFKSVSKALIYEQERHMQLAKENK
IGEKETRRWDEDTQTTIVMRHKEEGNDYRFSVEGDIPKTYVEQSYIEEIKKNLPELPEMRKERFMNEYKIDEYDADILTR
NGYLADYYEKVVEISNDPVQSSNWLLGDVLRQVNENEIEIEEMNMSAENLAKLIKLSSAKKINNQTAKKVLREMFNENFD
PEVYVKEKGLLQVDDDNLLQQIVDEVVAENPESIESIRNGKDRAIGFLVGQCMKKSKGKGNPQKFNELIKEKI
>Mature_473_residues
MNLKTLIGLEIHVELSTKTKMFCGCKNEFGQIPNTNVCPICLGHPGALPHMNKQALRYAIMAGLAFDCDIANEFKMDRKK
YFYPDLVKGYQITQEDQPLCTNGYIELKTSTKNKKVRIRRIHIEEDTGKSIHNESGNTLMDYNRAGVPLIEIVSEPDMSN
PDEAREFLETLRERIKYLEISDVKMSEGSLRCDVNINVYDEDSDFKTKISEIKNLNSFKSVSKALIYEQERHMQLAKENK
IGEKETRRWDEDTQTTIVMRHKEEGNDYRFSVEGDIPKTYVEQSYIEEIKKNLPELPEMRKERFMNEYKIDEYDADILTR
NGYLADYYEKVVEISNDPVQSSNWLLGDVLRQVNENEIEIEEMNMSAENLAKLIKLSSAKKINNQTAKKVLREMFNENFD
PEVYVKEKGLLQVDDDNLLQQIVDEVVAENPESIESIRNGKDRAIGFLVGQCMKKSKGKGNPQKFNELIKEKI

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=492, Percent_Identity=32.1138211382114, Blast_Score=260, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI115534094, Length=302, Percent_Identity=34.7682119205298, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=514, Percent_Identity=27.6264591439689, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=29.9595141700405, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_FINM2 (B0RZW3)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691880.1
- ProteinModelPortal:   B0RZW3
- SMR:   B0RZW3
- GeneID:   6019310
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0572
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   CLSK2541990
- BioCyc:   FMAG334413:FMG_0572-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54752; Mature: 54752

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKTLIGLEIHVELSTKTKMFCGCKNEFGQIPNTNVCPICLGHPGALPHMNKQALRYAI
CCCCEEEEEEEEEEECCCCEEEECCCHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
MAGLAFDCDIANEFKMDRKKYFYPDLVKGYQITQEDQPLCTNGYIELKTSTKNKKVRIRR
HHCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
IHIEEDTGKSIHNESGNTLMDYNRAGVPLIEIVSEPDMSNPDEAREFLETLRERIKYLEI
EEEECCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
SDVKMSEGSLRCDVNINVYDEDSDFKTKISEIKNLNSFKSVSKALIYEQERHMQLAKENK
CCCEECCCCEEEEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
IGEKETRRWDEDTQTTIVMRHKEEGNDYRFSVEGDIPKTYVEQSYIEEIKKNLPELPEMR
CCCHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
KERFMNEYKIDEYDADILTRNGYLADYYEKVVEISNDPVQSSNWLLGDVLRQVNENEIEI
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEE
EEMNMSAENLAKLIKLSSAKKINNQTAKKVLREMFNENFDPEVYVKEKGLLQVDDDNLLQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHH
QIVDEVVAENPESIESIRNGKDRAIGFLVGQCMKKSKGKGNPQKFNELIKEKI
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNLKTLIGLEIHVELSTKTKMFCGCKNEFGQIPNTNVCPICLGHPGALPHMNKQALRYAI
CCCCEEEEEEEEEEECCCCEEEECCCHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHH
MAGLAFDCDIANEFKMDRKKYFYPDLVKGYQITQEDQPLCTNGYIELKTSTKNKKVRIRR
HHCCCCCCCCCHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
IHIEEDTGKSIHNESGNTLMDYNRAGVPLIEIVSEPDMSNPDEAREFLETLRERIKYLEI
EEEECCCCCCCCCCCCCEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
SDVKMSEGSLRCDVNINVYDEDSDFKTKISEIKNLNSFKSVSKALIYEQERHMQLAKENK
CCCEECCCCEEEEEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
IGEKETRRWDEDTQTTIVMRHKEEGNDYRFSVEGDIPKTYVEQSYIEEIKKNLPELPEMR
CCCHHHHCCCCCCCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
KERFMNEYKIDEYDADILTRNGYLADYYEKVVEISNDPVQSSNWLLGDVLRQVNENEIEI
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEE
EEMNMSAENLAKLIKLSSAKKINNQTAKKVLREMFNENFDPEVYVKEKGLLQVDDDNLLQ
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCHHHHH
QIVDEVVAENPESIESIRNGKDRAIGFLVGQCMKKSKGKGNPQKFNELIKEKI
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA