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The map label for this gene is gatA2 [H]

Identifier: 169824268

GI number: 169824268

Start: 634596

End: 635987

Strand: Direct

Name: gatA2 [H]

Synonym: FMG_0571

Alternate gene names: 169824268

Gene position: 634596-635987 (Clockwise)

Preceding gene: 169824267

Following gene: 169824269

Centisome position: 35.3

GC content: 31.68

Gene sequence:

>1392_bases
ATGATAGAAAATCTTAGAAACAAATTTGTAGAAAATCCTGAAAGTTTAAGGGACTACTACAATGATTTGGATAAATTAAT
AGAATCAAAAAAAGATTTGAATGTGTTCATAACTTACGACAAATCATTCGTCGATGAACAAATTGAAAAACTAATTGAAA
AAGCAAAAAACAACGAAAATTTGGGAAAAATGTTCGGCGTTGCAGTTAGTGTTAAGGACAACATCAATGTAAAAGATTTG
AAGATGACTTGTGGATCAAAAATGTTAGAGGATTATGTTTCAATTTATGATGCCACTGTAATCAAAAAATTAAAAGAAGA
AGACTGCGTTATAGTTGGAAAAGTTAATATGGATGAATTTGCGATGGGATCTTCTTCAGAAACAAGTTATTTTGGCCCTA
CAAAAAATCCAGTAGACACAAAATTAATCCCAGGTGGTTCTTCATCAGGTTCAGCTGCCAGTATCAAAGCTGAAATGGCT
GTTGTAAGTTTAGGAACTGATACAGGTGGATCTAGTAGACAACCGGCATCTTGTTGTAATGTTGTTGGTTTTATGCCAAC
GTATGGTTCAATTTCAAGATTCGGTGTTCAATCAATGGCAAATACATTGGATGAAGTTGGTATATTATCAAATTCTGTTG
AAGACATAATGGATACTTACAATGTAATTGCAGGCCACGATGACAATGATATGACCACAATACAATCTGAAGTAAATGTT
AAAAAGAAAGATTATGACTTCAAAGGCAAAAAAATTGCCGTTGTTGATTTGAAAAAATATAGCAATGATGAGACAGTTAT
CAAAGAATACAAAGAAATATTAGAAATTCTAAAAAATATGGGAGCAGATTTAGTTGAAATCGACTTCGAATATTTAAAAT
ACGCAAATGCTTTATACAACGTAATCGTAACGAGTGAAGTATCATCTAATATGTCAAGATTTGATGGAATTAGATATGGA
TATTTGACAGATGATTATGAAAATACGAGAGATTTATTTGTTAAAGTTAGAAGTGAAGGATTTGGAGAAGAAGTTAAGAG
AAGAATTGCTATGGGAACTTTTTATCTAGCAAGTTCCAACGACCAAAAAGTTTACAAACAAGGTTTAAAAGTTAGAAATC
TGCTTTCACAAGAATTCAAAAATATCTTTAAAGAATTTGATTTAATCGCCACACCTACAATGACAAGCTTACCTTATGAA
TTAGACAGCAGAAAAGACGATCCATTGGCTGTGTATGATTCTGGTATTTTTAATGTAATTGTAAACTTGAGTGGATTGTG
TGCTATTTCAATTCCTTACAAATCAGGAATATCTACTAGTTTGCAACTAATTGCAAATAGTCACGACGAAGAAAATTTAT
TGAATGCAGCTTTAGCGTTTGAAAGGAGATAG

Upstream 100 bases:

>100_bases
GAGAAGATACACCAAAAGAATCAATTCCAAGAGATGTTGCGTTGAAGAATGCAAAATCTCGTGAGTATGGTTATTTTAAA
TTGAAGAAAGTGGTGGAATA

Downstream 100 bases:

>100_bases
CATGAACTTAAAAACACTTATAGGATTGGAAATTCACGTAGAACTATCAACTAAAACTAAAATGTTTTGTGGTTGTAAGA
ATGAGTTTGGACAAATCCCA

Product: glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A 2 [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MIENLRNKFVENPESLRDYYNDLDKLIESKKDLNVFITYDKSFVDEQIEKLIEKAKNNENLGKMFGVAVSVKDNINVKDL
KMTCGSKMLEDYVSIYDATVIKKLKEEDCVIVGKVNMDEFAMGSSSETSYFGPTKNPVDTKLIPGGSSSGSAASIKAEMA
VVSLGTDTGGSSRQPASCCNVVGFMPTYGSISRFGVQSMANTLDEVGILSNSVEDIMDTYNVIAGHDDNDMTTIQSEVNV
KKKDYDFKGKKIAVVDLKKYSNDETVIKEYKEILEILKNMGADLVEIDFEYLKYANALYNVIVTSEVSSNMSRFDGIRYG
YLTDDYENTRDLFVKVRSEGFGEEVKRRIAMGTFYLASSNDQKVYKQGLKVRNLLSQEFKNIFKEFDLIATPTMTSLPYE
LDSRKDDPLAVYDSGIFNVIVNLSGLCAISIPYKSGISTSLQLIANSHDEENLLNAALAFERR

Sequences:

>Translated_463_residues
MIENLRNKFVENPESLRDYYNDLDKLIESKKDLNVFITYDKSFVDEQIEKLIEKAKNNENLGKMFGVAVSVKDNINVKDL
KMTCGSKMLEDYVSIYDATVIKKLKEEDCVIVGKVNMDEFAMGSSSETSYFGPTKNPVDTKLIPGGSSSGSAASIKAEMA
VVSLGTDTGGSSRQPASCCNVVGFMPTYGSISRFGVQSMANTLDEVGILSNSVEDIMDTYNVIAGHDDNDMTTIQSEVNV
KKKDYDFKGKKIAVVDLKKYSNDETVIKEYKEILEILKNMGADLVEIDFEYLKYANALYNVIVTSEVSSNMSRFDGIRYG
YLTDDYENTRDLFVKVRSEGFGEEVKRRIAMGTFYLASSNDQKVYKQGLKVRNLLSQEFKNIFKEFDLIATPTMTSLPYE
LDSRKDDPLAVYDSGIFNVIVNLSGLCAISIPYKSGISTSLQLIANSHDEENLLNAALAFERR
>Mature_463_residues
MIENLRNKFVENPESLRDYYNDLDKLIESKKDLNVFITYDKSFVDEQIEKLIEKAKNNENLGKMFGVAVSVKDNINVKDL
KMTCGSKMLEDYVSIYDATVIKKLKEEDCVIVGKVNMDEFAMGSSSETSYFGPTKNPVDTKLIPGGSSSGSAASIKAEMA
VVSLGTDTGGSSRQPASCCNVVGFMPTYGSISRFGVQSMANTLDEVGILSNSVEDIMDTYNVIAGHDDNDMTTIQSEVNV
KKKDYDFKGKKIAVVDLKKYSNDETVIKEYKEILEILKNMGADLVEIDFEYLKYANALYNVIVTSEVSSNMSRFDGIRYG
YLTDDYENTRDLFVKVRSEGFGEEVKRRIAMGTFYLASSNDQKVYKQGLKVRNLLSQEFKNIFKEFDLIATPTMTSLPYE
LDSRKDDPLAVYDSGIFNVIVNLSGLCAISIPYKSGISTSLQLIANSHDEENLLNAALAFERR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=471, Percent_Identity=34.8195329087049, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI195972892, Length=268, Percent_Identity=25, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=427, Percent_Identity=31.8501170960187, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI71990152, Length=145, Percent_Identity=33.7931034482759, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=156, Percent_Identity=30.1282051282051, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17537465, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17538252, Length=186, Percent_Identity=26.8817204301075, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=35.2517985611511, Blast_Score=213, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6319685, Length=425, Percent_Identity=27.5294117647059, Blast_Score=107, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648113, Length=457, Percent_Identity=34.7921225382932, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24652985, Length=473, Percent_Identity=22.1987315010571, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922090, Length=473, Percent_Identity=22.1987315010571, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652981, Length=473, Percent_Identity=22.1987315010571, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24652983, Length=473, Percent_Identity=22.1987315010571, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356731, Length=293, Percent_Identity=27.9863481228669, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI45550774, Length=149, Percent_Identity=32.2147651006711, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24648435, Length=149, Percent_Identity=32.2147651006711, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648437, Length=149, Percent_Identity=32.2147651006711, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648441, Length=141, Percent_Identity=32.6241134751773, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648439, Length=141, Percent_Identity=32.6241134751773, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51747; Mature: 51747

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIENLRNKFVENPESLRDYYNDLDKLIESKKDLNVFITYDKSFVDEQIEKLIEKAKNNEN
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCC
LGKMFGVAVSVKDNINVKDLKMTCGSKMLEDYVSIYDATVIKKLKEEDCVIVGKVNMDEF
CHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
AMGSSSETSYFGPTKNPVDTKLIPGGSSSGSAASIKAEMAVVSLGTDTGGSSRQPASCCN
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHH
VVGFMPTYGSISRFGVQSMANTLDEVGILSNSVEDIMDTYNVIAGHDDNDMTTIQSEVNV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCC
KKKDYDFKGKKIAVVDLKKYSNDETVIKEYKEILEILKNMGADLVEIDFEYLKYANALYN
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
VIVTSEVSSNMSRFDGIRYGYLTDDYENTRDLFVKVRSEGFGEEVKRRIAMGTFYLASSN
HHEEHHHHHHHHHHCCEEEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHEEEEECCC
DQKVYKQGLKVRNLLSQEFKNIFKEFDLIATPTMTSLPYELDSRKDDPLAVYDSGIFNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHH
VNLSGLCAISIPYKSGISTSLQLIANSHDEENLLNAALAFERR
HCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MIENLRNKFVENPESLRDYYNDLDKLIESKKDLNVFITYDKSFVDEQIEKLIEKAKNNEN
CCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCC
LGKMFGVAVSVKDNINVKDLKMTCGSKMLEDYVSIYDATVIKKLKEEDCVIVGKVNMDEF
CHHHHEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
AMGSSSETSYFGPTKNPVDTKLIPGGSSSGSAASIKAEMAVVSLGTDTGGSSRQPASCCN
CCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHH
VVGFMPTYGSISRFGVQSMANTLDEVGILSNSVEDIMDTYNVIAGHDDNDMTTIQSEVNV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHCCC
KKKDYDFKGKKIAVVDLKKYSNDETVIKEYKEILEILKNMGADLVEIDFEYLKYANALYN
CCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHH
VIVTSEVSSNMSRFDGIRYGYLTDDYENTRDLFVKVRSEGFGEEVKRRIAMGTFYLASSN
HHEEHHHHHHHHHHCCEEEECCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHEEEEECCC
DQKVYKQGLKVRNLLSQEFKNIFKEFDLIATPTMTSLPYELDSRKDDPLAVYDSGIFNVI
CHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCCCCCCCCCCCCCCCCCEEEECCHHHHHH
VNLSGLCAISIPYKSGISTSLQLIANSHDEENLLNAALAFERR
HCCCCEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]