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The map label for this gene is ligA

Identifier: 169824266

GI number: 169824266

Start: 632276

End: 634264

Strand: Direct

Name: ligA

Synonym: FMG_0569

Alternate gene names: 169824266

Gene position: 632276-634264 (Clockwise)

Preceding gene: 169824265

Following gene: 169824267

Centisome position: 35.17

GC content: 30.92

Gene sequence:

>1989_bases
ATGGAAAAACAAGATAGAATAAAATATTTAGTAGACATACTAAATAAATACGCTTACCATTATTACACTTTGGATGATCC
ATTGATTGCGGATTCAGAGTACGATGTTTTGTATGATGAGCTTGTAAATCTAGAAAAAGAAACTGGAATTGTTCTACCAA
ATTCTCCTACAAATAGAATAGGTGGAGAAGTTTTATCAGGGTTTGAAAAGCATGAGCATTTGAATACTTTGTACAGTTTA
GGAAAAGCGCAAAGCAAAGAAGAAGTTAGAAATTGGGTAGATAAAACCATTGAGTTTGTACAAAATTACAATGAAAATCA
CGTAAACAAATTACCGAAAGTTGAATTTTATGTAGAATTTAAGTTTGACGGATTGACAGTGAACTTAACTTATGATGGTG
GATATTTGGTTATGGCTACAACAAGAGGAAACGGTACTATTGGAGAAGTTATTACAAGTCAAGTAAAAACTATTAATTCA
ATTCCATTGAAAATAAAAGATACAAGATTAATGGAAATTCAAGGTGAAGGAGTCATGCCACTTTCTTCATTAGAAAACTA
CAATAAAACTCATGAACCAAAACTAAAAAATGCAAGAAATGCTGCAGCGGGAGCCCTTAGAAACTTAGATCCAGCTGTTA
CTAGAGAGAGAAATTTGGATGCTTATTTCTATAATGTAAATTATTTACAAGATAACGAGCTTGAAACTCAAGAAGAAATG
ATTAAGTTTTTGGGAGATAATTATTTTAAATTGTATCCATACGAAAAACACGCTACATCATTTGAAGAAGTTTCAAAATT
TATTGACGAGATTTTTGAATTGAGAAATGAAATTGACGTGTTGACAGATGGTGTGGTAATTAAAGTTAATGATTTTAAAA
CAAGAGAAAAATTAGGATACACTAATAAATTTCCAAGATGGGCAATAGCTTACAAATTTGAACCTGAAAGATTTACAACA
ATTGTAAAAGAAGTAGAATGGAATGTTGGAAGAACTGGAAAAGTGACTCCGACAGCTTTGCTGGAGCCTGTAGAGATTGG
AAATGTAACTGTAAAAAGGGCTACTCTGAATAATATTGACGATATTGAAAGAAAACAAGTGAGATTAAATTCAGAAGTTT
TTGTTAGAAGGTCAAACGATGTAATTCCAGAAATAATGGGAGTTGTAAACCCTGACCAAGAAGATACGGAAGAAATTGAA
ATTCCGCATTACTGTCCGTATTGCCACAGTGAATTATTCAGAGATGGAGTTCATATCTTCTGCCCAAATTCTATGAGTTG
TACTCCGCAATTGGTTAAGAGAATGGTTCATTTTGCAAGCAGAAATGCGATGAATATAGATGGATTGAGTGAAAAAACAT
TGGAAGTTTTGCTAGAAAAACTTAATATAAAATCAATTGAAGAAATCTACGATGTAAAAAAAGAACAGTTGATGCAATTA
GATGGTTTTAAAGAAAAGAAGAGCCAAAATCTTATAAATGCAATTGAAAAAAGTAAAAATGTGGATTTAGCTAATTTTAT
TTTTGCACTTGGAATTCCTGAAATTGGAGAGAAAACAAGCTTTGAATTGGCACAAAAATATAAAAGCTTTGATAATTTAA
GAGAGGCGAAGTTTGAAGAATTAATTCAAATCGAAGATATAGGAAATGTTATAGCAGAAGAAATCGTAGAATTTTTCCAT
GACGAAACTATTTCTAATTCTATCGATTCACTTCTATCAAAAGGAATTAAAATCAAAAATCCTGAAAATATTGAAAAGAA
ATTGGATAATTTAACTTTTGTGTTGACGGGAAGCTTAGTAAATTATTCTAGAAAAGAACTTACAGATAAGTTATCAAATT
TGGGAGCAAAAGTTTCATCATCTGTATCCAAAAACACTGATTACGTTGTATATGGTGAAAAAGCAGGATCCAAACTTACA
AAAGCGAAGGATTTAGGTGTAAAATTAATGACAGAAGATGAATTAAATTCGTTCTTGGACAACTTATAA

Upstream 100 bases:

>100_bases
TTGTAGAAAAAGAAAATGGAGATGAATTGACAATTAATTTTGACAAGAAAGGGTTGAAGAAACTAAATTCTTCATTGGCA
CCACTAACAAGGGTTGAATA

Downstream 100 bases:

>100_bases
ATTTAATTTATTTATGATAAAATTTAATCATGAATTAAAAAGGAGTTAGTATGATTAATCGCGATGAAGTAGTAAAAATC
TATGATTTGGCTAACCTTGA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MEKQDRIKYLVDILNKYAYHYYTLDDPLIADSEYDVLYDELVNLEKETGIVLPNSPTNRIGGEVLSGFEKHEHLNTLYSL
GKAQSKEEVRNWVDKTIEFVQNYNENHVNKLPKVEFYVEFKFDGLTVNLTYDGGYLVMATTRGNGTIGEVITSQVKTINS
IPLKIKDTRLMEIQGEGVMPLSSLENYNKTHEPKLKNARNAAAGALRNLDPAVTRERNLDAYFYNVNYLQDNELETQEEM
IKFLGDNYFKLYPYEKHATSFEEVSKFIDEIFELRNEIDVLTDGVVIKVNDFKTREKLGYTNKFPRWAIAYKFEPERFTT
IVKEVEWNVGRTGKVTPTALLEPVEIGNVTVKRATLNNIDDIERKQVRLNSEVFVRRSNDVIPEIMGVVNPDQEDTEEIE
IPHYCPYCHSELFRDGVHIFCPNSMSCTPQLVKRMVHFASRNAMNIDGLSEKTLEVLLEKLNIKSIEEIYDVKKEQLMQL
DGFKEKKSQNLINAIEKSKNVDLANFIFALGIPEIGEKTSFELAQKYKSFDNLREAKFEELIQIEDIGNVIAEEIVEFFH
DETISNSIDSLLSKGIKIKNPENIEKKLDNLTFVLTGSLVNYSRKELTDKLSNLGAKVSSSVSKNTDYVVYGEKAGSKLT
KAKDLGVKLMTEDELNSFLDNL

Sequences:

>Translated_662_residues
MEKQDRIKYLVDILNKYAYHYYTLDDPLIADSEYDVLYDELVNLEKETGIVLPNSPTNRIGGEVLSGFEKHEHLNTLYSL
GKAQSKEEVRNWVDKTIEFVQNYNENHVNKLPKVEFYVEFKFDGLTVNLTYDGGYLVMATTRGNGTIGEVITSQVKTINS
IPLKIKDTRLMEIQGEGVMPLSSLENYNKTHEPKLKNARNAAAGALRNLDPAVTRERNLDAYFYNVNYLQDNELETQEEM
IKFLGDNYFKLYPYEKHATSFEEVSKFIDEIFELRNEIDVLTDGVVIKVNDFKTREKLGYTNKFPRWAIAYKFEPERFTT
IVKEVEWNVGRTGKVTPTALLEPVEIGNVTVKRATLNNIDDIERKQVRLNSEVFVRRSNDVIPEIMGVVNPDQEDTEEIE
IPHYCPYCHSELFRDGVHIFCPNSMSCTPQLVKRMVHFASRNAMNIDGLSEKTLEVLLEKLNIKSIEEIYDVKKEQLMQL
DGFKEKKSQNLINAIEKSKNVDLANFIFALGIPEIGEKTSFELAQKYKSFDNLREAKFEELIQIEDIGNVIAEEIVEFFH
DETISNSIDSLLSKGIKIKNPENIEKKLDNLTFVLTGSLVNYSRKELTDKLSNLGAKVSSSVSKNTDYVVYGEKAGSKLT
KAKDLGVKLMTEDELNSFLDNL
>Mature_662_residues
MEKQDRIKYLVDILNKYAYHYYTLDDPLIADSEYDVLYDELVNLEKETGIVLPNSPTNRIGGEVLSGFEKHEHLNTLYSL
GKAQSKEEVRNWVDKTIEFVQNYNENHVNKLPKVEFYVEFKFDGLTVNLTYDGGYLVMATTRGNGTIGEVITSQVKTINS
IPLKIKDTRLMEIQGEGVMPLSSLENYNKTHEPKLKNARNAAAGALRNLDPAVTRERNLDAYFYNVNYLQDNELETQEEM
IKFLGDNYFKLYPYEKHATSFEEVSKFIDEIFELRNEIDVLTDGVVIKVNDFKTREKLGYTNKFPRWAIAYKFEPERFTT
IVKEVEWNVGRTGKVTPTALLEPVEIGNVTVKRATLNNIDDIERKQVRLNSEVFVRRSNDVIPEIMGVVNPDQEDTEEIE
IPHYCPYCHSELFRDGVHIFCPNSMSCTPQLVKRMVHFASRNAMNIDGLSEKTLEVLLEKLNIKSIEEIYDVKKEQLMQL
DGFKEKKSQNLINAIEKSKNVDLANFIFALGIPEIGEKTSFELAQKYKSFDNLREAKFEELIQIEDIGNVIAEEIVEFFH
DETISNSIDSLLSKGIKIKNPENIEKKLDNLTFVLTGSLVNYSRKELTDKLSNLGAKVSSSVSKNTDYVVYGEKAGSKLT
KAKDLGVKLMTEDELNSFLDNL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=677, Percent_Identity=38.109305760709, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI87082305, Length=455, Percent_Identity=23.7362637362637, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_FINM2 (B0RZW0)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691877.1
- ProteinModelPortal:   B0RZW0
- GeneID:   6019314
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0569
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   FMAG334413:FMG_0569-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75906; Mature: 75906

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 121-121 BINDING 119-119 BINDING 142-142 BINDING 176-176 BINDING 288-288 BINDING 312-312

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKQDRIKYLVDILNKYAYHYYTLDDPLIADSEYDVLYDELVNLEKETGIVLPNSPTNRI
CCHHHHHHHHHHHHHHHHEEEEECCCCEECCCCHHHHHHHHHCCHHHCCEEECCCCCHHH
GGEVLSGFEKHEHLNTLYSLGKAQSKEEVRNWVDKTIEFVQNYNENHVNKLPKVEFYVEF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEE
KFDGLTVNLTYDGGYLVMATTRGNGTIGEVITSQVKTINSIPLKIKDTRLMEIQGEGVMP
EECCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCC
LSSLENYNKTHEPKLKNARNAAAGALRNLDPAVTRERNLDAYFYNVNYLQDNELETQEEM
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCHHHHH
IKFLGDNYFKLYPYEKHATSFEEVSKFIDEIFELRNEIDVLTDGVVIKVNDFKTREKLGY
HHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
TNKFPRWAIAYKFEPERFTTIVKEVEWNVGRTGKVTPTALLEPVEIGNVTVKRATLNNID
CCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEECCCEEEEECCCCCCH
DIERKQVRLNSEVFVRRSNDVIPEIMGVVNPDQEDTEEIEIPHYCPYCHSELFRDGVHIF
HHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
CPNSMSCTPQLVKRMVHFASRNAMNIDGLSEKTLEVLLEKLNIKSIEEIYDVKKEQLMQL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DGFKEKKSQNLINAIEKSKNVDLANFIFALGIPEIGEKTSFELAQKYKSFDNLREAKFEE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIQIEDIGNVIAEEIVEFFHDETISNSIDSLLSKGIKIKNPENIEKKLDNLTFVLTGSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEECHHH
NYSRKELTDKLSNLGAKVSSSVSKNTDYVVYGEKAGSKLTKAKDLGVKLMTEDELNSFLD
HHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEEEHHHHHHHHH
NL
CC
>Mature Secondary Structure
MEKQDRIKYLVDILNKYAYHYYTLDDPLIADSEYDVLYDELVNLEKETGIVLPNSPTNRI
CCHHHHHHHHHHHHHHHHEEEEECCCCEECCCCHHHHHHHHHCCHHHCCEEECCCCCHHH
GGEVLSGFEKHEHLNTLYSLGKAQSKEEVRNWVDKTIEFVQNYNENHVNKLPKVEFYVEF
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEEEE
KFDGLTVNLTYDGGYLVMATTRGNGTIGEVITSQVKTINSIPLKIKDTRLMEIQGEGVMP
EECCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCCCCC
LSSLENYNKTHEPKLKNARNAAAGALRNLDPAVTRERNLDAYFYNVNYLQDNELETQEEM
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCHHHHH
IKFLGDNYFKLYPYEKHATSFEEVSKFIDEIFELRNEIDVLTDGVVIKVNDFKTREKLGY
HHHHCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHCCC
TNKFPRWAIAYKFEPERFTTIVKEVEWNVGRTGKVTPTALLEPVEIGNVTVKRATLNNID
CCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEECCCEEEEECCCCCCH
DIERKQVRLNSEVFVRRSNDVIPEIMGVVNPDQEDTEEIEIPHYCPYCHSELFRDGVHIF
HHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEE
CPNSMSCTPQLVKRMVHFASRNAMNIDGLSEKTLEVLLEKLNIKSIEEIYDVKKEQLMQL
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
DGFKEKKSQNLINAIEKSKNVDLANFIFALGIPEIGEKTSFELAQKYKSFDNLREAKFEE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LIQIEDIGNVIAEEIVEFFHDETISNSIDSLLSKGIKIKNPENIEKKLDNLTFVLTGSLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCEEEEEECHHH
NYSRKELTDKLSNLGAKVSSSVSKNTDYVVYGEKAGSKLTKAKDLGVKLMTEDELNSFLD
HHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEEEHHHHHHHHH
NL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA