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The map label for this gene is pcrA [H]

Identifier: 169824265

GI number: 169824265

Start: 630099

End: 632276

Strand: Direct

Name: pcrA [H]

Synonym: FMG_0568

Alternate gene names: 169824265

Gene position: 630099-632276 (Clockwise)

Preceding gene: 169824264

Following gene: 169824266

Centisome position: 35.05

GC content: 31.91

Gene sequence:

>2178_bases
ATGATGAATTTAGATAATTTAAATGATAGACAGAGAGAAGCCGTCGAAAATACAGATGGTCCTATGTTGATATTGGCAGG
AGCTGGAAGTGGTAAAACAAAAGTGTTGACCACTAAAATAGCTTATTGTTTGGAGAAAGGATTGTGTTCAAAATACGAAA
TTTTGGCAATTACTTTTACAAACAAAGCTGCCAAAGAAATGAAAGAAAGGGTCGAAAATATTCTGCAAGAAAACGTCGAT
AGTATGTGGATAGGAACTTTCCATGCTATTTGTTCTAGAATTTTGAGAGTAGAAATAGAAAGAATTGGATTCGAAAGAAA
CTTCACCGTTTACGACAGAGCAGATCAAATATCTCTTGTAAAAGAATGTTTGAAGGATATGGGTGTTGATCCTAAAAATG
TAGAAATCAAAAACGAAATCAATACAATTTCTAGATTGAAAAACGCAGGTGCTACGGAAAAAAATATTGACAAAGAAGAT
TTTGATATAAGTTTAAGCACTTTTACGATGGAATTATTTCAAAAATACGAGCACAAATTAAAAAGGTACAATTCGTTGGA
CTTTGATGATTTGATCGTGAAAACAAATTTATTGTTCGAGGAAAATCCAGACTTGAGAGAAAAATATTCTGACAGATTTA
AGTATGTATTTGTCGATGAATACCAAGACACTAACGATACACAATACAAGCTAATCAAGAATTTGGTTTCAAAAAACGAT
AATATTTGTTGCGTGGGAGATTCTGACCAATCAATTTACGGTTGGAGAGGAGCAAACATTCAAAACATTCAAAATTTTGA
AAAAGATTTTAAGAATGCTAAGGTTATTTTGTTGGAACAAAATTATCGTTCAACACAACCTATTTTGGACTTGGCAAACA
CTGTGATTAAAAACAATTCACTTAGAAAAGATAAAAATCTGTGGACTGCAAAAAACGAAGGGGATATGCCAATTTATAGG
AGAATGTATTCCGATATAGATGAGGCAGACCAAGTTGTTCAATGGATTGAACAAGAAAGATACCGAGAAAATCCATACGA
AGAAATGGCAATTTTGTACAGAACCAATGCACAATCTAGACTTTTCGAAGAAAGATTAAATAGACTTGGAATTCCAAATA
GAGTTGTAGGTGGATTGAAATTCTACGATAGAAAAGAAATCAAAGACTGCGTTAGTTATCTTAGAATCGTTGAAAATTTG
AACGATAACATGGCATTAAATAGAATTATCAATGAACCAAAAAGAGGGATTGGTAAAACAACAATGGATAAGTTGTTGCA
ATGTTCTCAAAACAGATCAATTAGCATGATGCAATACATCATAGATACAGATCTTCCTGATTTTTCAAATGCTACAGCGA
AAAAGCTTCAAGAGTTTTATTATTTAATCAAAAGCTTTCCAAAAGATCAAATGAATGTTGCAGAACTTATGGAATATATT
TTGGATAAAACAGGATACAGAGCTAATCTAGAAAAATCAACTAATAGAGATGATAAAACGAGGTTGGAAAATATTGATGA
ATATGTTTCATCACTATATCAATTTGTTCAAGATAATCCAGATAAAAACTTGAGAGAATATTTGGAAACTTCTTCATTAA
TGACTGATTTGGATAAAACTGATGATAATACAAAAGGTGTATCAATGATGACAATCCACGCTGCAAAAGGCTTGGAGTTT
GATGTTGTATTTTTTACAGGACTTGAGGAAGGGACTATTCCATCTAGAGTTGATGAAGATGTAGAAGAAGAAAGAAGATT
GTGCTACGTTGCAATCACTAGAGCAAGAAAGAAATTGTACATAACAAGTGTGCAATCGAGAAGAAGATTTAATGAATTTC
AAACAAAAGCTGAATCGAGGTTTATAGAGGAAATGGAAAATAAATACAAGGACGAATCTCCTAATAAAGAGGTTAGTTTT
AGAGAATCGTTCAACACAAAAGTAGTTGATGATTACAGAACAGAAGCATCTGCTTCAATCACTAAAAAACAAAATAATGT
TACTAAAAATAACGAAAAATATAGAGCTGGAGACAAAGTTTCACATAAAAAGTTTGGAACTGGTGTAATCGTTGGAATTG
TAGAAAAAGAAAATGGAGATGAATTGACAATTAATTTTGACAAGAAAGGGTTGAAGAAACTAAATTCTTCATTGGCACCA
CTAACAAGGGTTGAATAA

Upstream 100 bases:

>100_bases
GATTGTAATTAATCCAAGAAGAACATTAGAAAACATATACGAATATATATTTAAAAAATAAGTTGTAGCGACTTATTTTT
TTATTGATACATAAGGAGTT

Downstream 100 bases:

>100_bases
TGGAAAAACAAGATAGAATAAAATATTTAGTAGACATACTAAATAAATACGCTTACCATTATTACACTTTGGATGATCCA
TTGATTGCGGATTCAGAGTA

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 725; Mature: 725

Protein sequence:

>725_residues
MMNLDNLNDRQREAVENTDGPMLILAGAGSGKTKVLTTKIAYCLEKGLCSKYEILAITFTNKAAKEMKERVENILQENVD
SMWIGTFHAICSRILRVEIERIGFERNFTVYDRADQISLVKECLKDMGVDPKNVEIKNEINTISRLKNAGATEKNIDKED
FDISLSTFTMELFQKYEHKLKRYNSLDFDDLIVKTNLLFEENPDLREKYSDRFKYVFVDEYQDTNDTQYKLIKNLVSKND
NICCVGDSDQSIYGWRGANIQNIQNFEKDFKNAKVILLEQNYRSTQPILDLANTVIKNNSLRKDKNLWTAKNEGDMPIYR
RMYSDIDEADQVVQWIEQERYRENPYEEMAILYRTNAQSRLFEERLNRLGIPNRVVGGLKFYDRKEIKDCVSYLRIVENL
NDNMALNRIINEPKRGIGKTTMDKLLQCSQNRSISMMQYIIDTDLPDFSNATAKKLQEFYYLIKSFPKDQMNVAELMEYI
LDKTGYRANLEKSTNRDDKTRLENIDEYVSSLYQFVQDNPDKNLREYLETSSLMTDLDKTDDNTKGVSMMTIHAAKGLEF
DVVFFTGLEEGTIPSRVDEDVEEERRLCYVAITRARKKLYITSVQSRRRFNEFQTKAESRFIEEMENKYKDESPNKEVSF
RESFNTKVVDDYRTEASASITKKQNNVTKNNEKYRAGDKVSHKKFGTGVIVGIVEKENGDELTINFDKKGLKKLNSSLAP
LTRVE

Sequences:

>Translated_725_residues
MMNLDNLNDRQREAVENTDGPMLILAGAGSGKTKVLTTKIAYCLEKGLCSKYEILAITFTNKAAKEMKERVENILQENVD
SMWIGTFHAICSRILRVEIERIGFERNFTVYDRADQISLVKECLKDMGVDPKNVEIKNEINTISRLKNAGATEKNIDKED
FDISLSTFTMELFQKYEHKLKRYNSLDFDDLIVKTNLLFEENPDLREKYSDRFKYVFVDEYQDTNDTQYKLIKNLVSKND
NICCVGDSDQSIYGWRGANIQNIQNFEKDFKNAKVILLEQNYRSTQPILDLANTVIKNNSLRKDKNLWTAKNEGDMPIYR
RMYSDIDEADQVVQWIEQERYRENPYEEMAILYRTNAQSRLFEERLNRLGIPNRVVGGLKFYDRKEIKDCVSYLRIVENL
NDNMALNRIINEPKRGIGKTTMDKLLQCSQNRSISMMQYIIDTDLPDFSNATAKKLQEFYYLIKSFPKDQMNVAELMEYI
LDKTGYRANLEKSTNRDDKTRLENIDEYVSSLYQFVQDNPDKNLREYLETSSLMTDLDKTDDNTKGVSMMTIHAAKGLEF
DVVFFTGLEEGTIPSRVDEDVEEERRLCYVAITRARKKLYITSVQSRRRFNEFQTKAESRFIEEMENKYKDESPNKEVSF
RESFNTKVVDDYRTEASASITKKQNNVTKNNEKYRAGDKVSHKKFGTGVIVGIVEKENGDELTINFDKKGLKKLNSSLAP
LTRVE
>Mature_725_residues
MMNLDNLNDRQREAVENTDGPMLILAGAGSGKTKVLTTKIAYCLEKGLCSKYEILAITFTNKAAKEMKERVENILQENVD
SMWIGTFHAICSRILRVEIERIGFERNFTVYDRADQISLVKECLKDMGVDPKNVEIKNEINTISRLKNAGATEKNIDKED
FDISLSTFTMELFQKYEHKLKRYNSLDFDDLIVKTNLLFEENPDLREKYSDRFKYVFVDEYQDTNDTQYKLIKNLVSKND
NICCVGDSDQSIYGWRGANIQNIQNFEKDFKNAKVILLEQNYRSTQPILDLANTVIKNNSLRKDKNLWTAKNEGDMPIYR
RMYSDIDEADQVVQWIEQERYRENPYEEMAILYRTNAQSRLFEERLNRLGIPNRVVGGLKFYDRKEIKDCVSYLRIVENL
NDNMALNRIINEPKRGIGKTTMDKLLQCSQNRSISMMQYIIDTDLPDFSNATAKKLQEFYYLIKSFPKDQMNVAELMEYI
LDKTGYRANLEKSTNRDDKTRLENIDEYVSSLYQFVQDNPDKNLREYLETSSLMTDLDKTDDNTKGVSMMTIHAAKGLEF
DVVFFTGLEEGTIPSRVDEDVEEERRLCYVAITRARKKLYITSVQSRRRFNEFQTKAESRFIEEMENKYKDESPNKEVSF
RESFNTKVVDDYRTEASASITKKQNNVTKNNEKYRAGDKVSHKKFGTGVIVGIVEKENGDELTINFDKKGLKKLNSSLAP
LTRVE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=731, Percent_Identity=36.2517099863201, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI48994965, Length=637, Percent_Identity=37.0486656200942, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1787196, Length=91, Percent_Identity=41.7582417582418, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322369, Length=459, Percent_Identity=30.9368191721133, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324477, Length=757, Percent_Identity=23.5138705416116, Blast_Score=106, Evalue=1e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 84455; Mature: 84455

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNLDNLNDRQREAVENTDGPMLILAGAGSGKTKVLTTKIAYCLEKGLCSKYEILAITFT
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAKEMKERVENILQENVDSMWIGTFHAICSRILRVEIERIGFERNFTVYDRADQISLV
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHH
KECLKDMGVDPKNVEIKNEINTISRLKNAGATEKNIDKEDFDISLSTFTMELFQKYEHKL
HHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KRYNSLDFDDLIVKTNLLFEENPDLREKYSDRFKYVFVDEYQDTNDTQYKLIKNLVSKND
HHCCCCCHHHHHHHHHEEECCCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
NICCVGDSDQSIYGWRGANIQNIQNFEKDFKNAKVILLEQNYRSTQPILDLANTVIKNNS
CEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
LRKDKNLWTAKNEGDMPIYRRMYSDIDEADQVVQWIEQERYRENPYEEMAILYRTNAQSR
CCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHEEEHEECCHHHH
LFEERLNRLGIPNRVVGGLKFYDRKEIKDCVSYLRIVENLNDNMALNRIINEPKRGIGKT
HHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCHH
TMDKLLQCSQNRSISMMQYIIDTDLPDFSNATAKKLQEFYYLIKSFPKDQMNVAELMEYI
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LDKTGYRANLEKSTNRDDKTRLENIDEYVSSLYQFVQDNPDKNLREYLETSSLMTDLDKT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DDNTKGVSMMTIHAAKGLEFDVVFFTGLEEGTIPSRVDEDVEEERRLCYVAITRARKKLY
CCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEHHHHHHHHHHEE
ITSVQSRRRFNEFQTKAESRFIEEMENKYKDESPNKEVSFRESFNTKVVDDYRTEASASI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHH
TKKQNNVTKNNEKYRAGDKVSHKKFGTGVIVGIVEKENGDELTINFDKKGLKKLNSSLAP
HHHHCCCCCCCCCCCCCCCCCHHHCCCEEEEEEEEECCCCEEEEEECHHHHHHHHHHCCC
LTRVE
CCCCC
>Mature Secondary Structure
MMNLDNLNDRQREAVENTDGPMLILAGAGSGKTKVLTTKIAYCLEKGLCSKYEILAITFT
CCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAKEMKERVENILQENVDSMWIGTFHAICSRILRVEIERIGFERNFTVYDRADQISLV
CHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHH
KECLKDMGVDPKNVEIKNEINTISRLKNAGATEKNIDKEDFDISLSTFTMELFQKYEHKL
HHHHHHCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHH
KRYNSLDFDDLIVKTNLLFEENPDLREKYSDRFKYVFVDEYQDTNDTQYKLIKNLVSKND
HHCCCCCHHHHHHHHHEEECCCCCHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCC
NICCVGDSDQSIYGWRGANIQNIQNFEKDFKNAKVILLEQNYRSTQPILDLANTVIKNNS
CEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
LRKDKNLWTAKNEGDMPIYRRMYSDIDEADQVVQWIEQERYRENPYEEMAILYRTNAQSR
CCCCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHEEEHEECCHHHH
LFEERLNRLGIPNRVVGGLKFYDRKEIKDCVSYLRIVENLNDNMALNRIINEPKRGIGKT
HHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCCHH
TMDKLLQCSQNRSISMMQYIIDTDLPDFSNATAKKLQEFYYLIKSFPKDQMNVAELMEYI
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
LDKTGYRANLEKSTNRDDKTRLENIDEYVSSLYQFVQDNPDKNLREYLETSSLMTDLDKT
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DDNTKGVSMMTIHAAKGLEFDVVFFTGLEEGTIPSRVDEDVEEERRLCYVAITRARKKLY
CCCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHEEHHHHHHHHHHEE
ITSVQSRRRFNEFQTKAESRFIEEMENKYKDESPNKEVSFRESFNTKVVDDYRTEASASI
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHH
TKKQNNVTKNNEKYRAGDKVSHKKFGTGVIVGIVEKENGDELTINFDKKGLKKLNSSLAP
HHHHCCCCCCCCCCCCCCCCCHHHCCCEEEEEEEEECCCCEEEEEECHHHHHHHHHHCCC
LTRVE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA