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The map label for this gene is pdhD [H]

Identifier: 169824163

GI number: 169824163

Start: 523774

End: 525183

Strand: Direct

Name: pdhD [H]

Synonym: FMG_0466

Alternate gene names: 169824163

Gene position: 523774-525183 (Clockwise)

Preceding gene: 169824162

Following gene: 169824164

Centisome position: 29.14

GC content: 33.69

Gene sequence:

>1410_bases
ATGACTAAAGATATCATTATTATCGGCGCTGGACCTGGTGGCTATGAAACGGCCATCAGGGCATGCCAGCTCGGTTTAAA
TGTAACTTTAATCGAAAAAGCTGAAGTAGGTGGTACATGCTTGAACAGAGGATGTATACCTACTAAAACTTTATGGAAAA
TTGCTGATTTGTATAAAGAAATTAGAGAATCAGAAACTTTTGGAATTGAAGTTAATGACCATAAATTATTAGCCGATAAA
ATTAAACATAGAAAAACGGAAATTATCGAAAGACTTAGAAGTGGTGTTGAATACCTTTTCAAAACTTACGATAATTTAAC
TTTTATTAGAGGAGAAGCTAGCTTTAAGGATAATAAAACTGTTGTTGTGAAGACTTTGGAAGGAGAAACTAAAGAACTCA
CAGCAGATAAAATTATCATAGCTACTGGATCCAAAGACTATAAGCCAACTAATATTGAAGGCATCGACCATCCTAGAGTT
TTGACATCAACAGGACTTTTGGAATTAGAAGAAATTCCAAAATCTATGGTTGTTATCGGAACTGGCGTAATAGGAATGGA
ATTTGCATCAATCTATTCACAATTTGGAACTGAAGTTACTGTAGTTGGTAACAAATTGTTGAAAACAGAAGATGGCGAAA
TTCAAAAACGTCTAAAATCAATTTTGAAGAGCGATACTTTGAAGTTTGTAACGGGAGTTTACGCTAAGAAAATTGTTGAA
GAAGACGGAAGATTAAAAATCATTAGTCAAAAAGTTGGCAAAGATAAATTCGAAGAAACTTATGCAGACTACGTTCTTGT
AGCGAGTGGTAGAAGCAGTAATACAGACAATCTTGGACTTGAAAACACAGATATTAAAACTGAAAATAATGCTATTGTTG
TAGATGAAAATCTTCAAACAAATGTTGAAGGAATTTACTCCATTGGTGATTGCGTATACAAGAATACTCAATTAGCACAC
GTAGCAAGTAACCAAGGTAAAAATCTTGTAAGAGAATTTTCTGGAAAAGATAGAAATATAAACATGGACATAGTTCCAGC
GGTTGTATTTACAGTTCCAGAAATTGCATCTGTAGGTCTAACTGAAGAAAAAGCAAAAGAACAAAACATTGATTATGTAA
CAAGCAAATTTATGTATCAAGCTAATGGTAAGGCATTGAGTTTGAATGCTACAGAAGGTTTTGTAAAGATAGTTGCTACA
AAAGATTTATCTAAGATTTTAGGTTGCCATATAATTGGTCATGATGCATCCACTATTATTCACTTCGCTGCAATCGCGAT
GAACAATAATGTTGGAGTAGAAGGCTTATCAGCAATGATTTACGCTCACCCAACTATTAGTGAAGTGTTCATGGATTCTG
TTGAACAATTAGAAGGATTATCTATCAATACGCCAAACCCTAACAAATAA

Upstream 100 bases:

>100_bases
AAGATATATTATTAGAAGCTCCTATTACAACTGTTGTTAGAAGACCAGATGAAACAGAAGCTGCTAAGAATTTAATATTG
ACTTTCAAAAAAGGAGAATA

Downstream 100 bases:

>100_bases
ATCAAATCCTCGTATTTAATTTCTATGAAGAAATTGATACGGGGATTTTTTAATGTTTACAAAAAGTCAAACATAAAGTT
TGATTTTTCGTAAACTTTAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTKDIIIIGAGPGGYETAIRACQLGLNVTLIEKAEVGGTCLNRGCIPTKTLWKIADLYKEIRESETFGIEVNDHKLLADK
IKHRKTEIIERLRSGVEYLFKTYDNLTFIRGEASFKDNKTVVVKTLEGETKELTADKIIIATGSKDYKPTNIEGIDHPRV
LTSTGLLELEEIPKSMVVIGTGVIGMEFASIYSQFGTEVTVVGNKLLKTEDGEIQKRLKSILKSDTLKFVTGVYAKKIVE
EDGRLKIISQKVGKDKFEETYADYVLVASGRSSNTDNLGLENTDIKTENNAIVVDENLQTNVEGIYSIGDCVYKNTQLAH
VASNQGKNLVREFSGKDRNINMDIVPAVVFTVPEIASVGLTEEKAKEQNIDYVTSKFMYQANGKALSLNATEGFVKIVAT
KDLSKILGCHIIGHDASTIIHFAAIAMNNNVGVEGLSAMIYAHPTISEVFMDSVEQLEGLSINTPNPNK

Sequences:

>Translated_469_residues
MTKDIIIIGAGPGGYETAIRACQLGLNVTLIEKAEVGGTCLNRGCIPTKTLWKIADLYKEIRESETFGIEVNDHKLLADK
IKHRKTEIIERLRSGVEYLFKTYDNLTFIRGEASFKDNKTVVVKTLEGETKELTADKIIIATGSKDYKPTNIEGIDHPRV
LTSTGLLELEEIPKSMVVIGTGVIGMEFASIYSQFGTEVTVVGNKLLKTEDGEIQKRLKSILKSDTLKFVTGVYAKKIVE
EDGRLKIISQKVGKDKFEETYADYVLVASGRSSNTDNLGLENTDIKTENNAIVVDENLQTNVEGIYSIGDCVYKNTQLAH
VASNQGKNLVREFSGKDRNINMDIVPAVVFTVPEIASVGLTEEKAKEQNIDYVTSKFMYQANGKALSLNATEGFVKIVAT
KDLSKILGCHIIGHDASTIIHFAAIAMNNNVGVEGLSAMIYAHPTISEVFMDSVEQLEGLSINTPNPNK
>Mature_468_residues
TKDIIIIGAGPGGYETAIRACQLGLNVTLIEKAEVGGTCLNRGCIPTKTLWKIADLYKEIRESETFGIEVNDHKLLADKI
KHRKTEIIERLRSGVEYLFKTYDNLTFIRGEASFKDNKTVVVKTLEGETKELTADKIIIATGSKDYKPTNIEGIDHPRVL
TSTGLLELEEIPKSMVVIGTGVIGMEFASIYSQFGTEVTVVGNKLLKTEDGEIQKRLKSILKSDTLKFVTGVYAKKIVEE
DGRLKIISQKVGKDKFEETYADYVLVASGRSSNTDNLGLENTDIKTENNAIVVDENLQTNVEGIYSIGDCVYKNTQLAHV
ASNQGKNLVREFSGKDRNINMDIVPAVVFTVPEIASVGLTEEKAKEQNIDYVTSKFMYQANGKALSLNATEGFVKIVATK
DLSKILGCHIIGHDASTIIHFAAIAMNNNVGVEGLSAMIYAHPTISEVFMDSVEQLEGLSINTPNPNK

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=36.1471861471861, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI50301238, Length=468, Percent_Identity=29.7008547008547, Blast_Score=166, Evalue=6e-41,
Organism=Homo sapiens, GI22035672, Length=459, Percent_Identity=28.7581699346405, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI33519430, Length=447, Percent_Identity=27.5167785234899, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519428, Length=447, Percent_Identity=27.5167785234899, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33519426, Length=447, Percent_Identity=27.5167785234899, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI148277071, Length=447, Percent_Identity=27.5167785234899, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI148277065, Length=447, Percent_Identity=27.5167785234899, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI291045266, Length=435, Percent_Identity=28.735632183908, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI291045268, Length=430, Percent_Identity=26.046511627907, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=32.5531914893617, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082354, Length=474, Percent_Identity=29.535864978903, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87081717, Length=457, Percent_Identity=30.6345733041575, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789915, Length=444, Percent_Identity=31.7567567567568, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=36.3238512035011, Blast_Score=261, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI17557007, Length=481, Percent_Identity=31.8087318087318, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI71983419, Length=356, Percent_Identity=32.0224719101124, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=356, Percent_Identity=32.0224719101124, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=26.0606060606061, Blast_Score=129, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321091, Length=466, Percent_Identity=36.480686695279, Blast_Score=256, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=31.6239316239316, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=27.5210084033613, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI21358499, Length=459, Percent_Identity=36.3834422657952, Blast_Score=270, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24640553, Length=473, Percent_Identity=28.3298097251586, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640549, Length=473, Percent_Identity=28.3298097251586, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24640551, Length=473, Percent_Identity=28.3298097251586, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=27.9749478079332, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51495; Mature: 51363

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKDIIIIGAGPGGYETAIRACQLGLNVTLIEKAEVGGTCLNRGCIPTKTLWKIADLYKE
CCCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHH
IRESETFGIEVNDHKLLADKIKHRKTEIIERLRSGVEYLFKTYDNLTFIRGEASFKDNKT
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCE
VVVKTLEGETKELTADKIIIATGSKDYKPTNIEGIDHPRVLTSTGLLELEEIPKSMVVIG
EEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCEEHHCCCEEHHHCCCEEEEEE
TGVIGMEFASIYSQFGTEVTVVGNKLLKTEDGEIQKRLKSILKSDTLKFVTGVYAKKIVE
CCHHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDGRLKIISQKVGKDKFEETYADYVLVASGRSSNTDNLGLENTDIKTENNAIVVDENLQT
CCCCEEEEHHHCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
NVEGIYSIGDCVYKNTQLAHVASNQGKNLVREFSGKDRNINMDIVPAVVFTVPEIASVGL
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCC
TEEKAKEQNIDYVTSKFMYQANGKALSLNATEGFVKIVATKDLSKILGCHIIGHDASTII
CHHHHHHCCCHHHHHHHEEECCCCEEEEECCCCEEEEEEHHHHHHHHCEEEECCCHHHHH
HFAAIAMNNNVGVEGLSAMIYAHPTISEVFMDSVEQLEGLSINTPNPNK
HEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKDIIIIGAGPGGYETAIRACQLGLNVTLIEKAEVGGTCLNRGCIPTKTLWKIADLYKE
CCCEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHCCCCCCHHHHHHHHHHHHH
IRESETFGIEVNDHKLLADKIKHRKTEIIERLRSGVEYLFKTYDNLTFIRGEASFKDNKT
HHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCE
VVVKTLEGETKELTADKIIIATGSKDYKPTNIEGIDHPRVLTSTGLLELEEIPKSMVVIG
EEEEEECCCCCEECCCEEEEEECCCCCCCCCCCCCCCCCEEHHCCCEEHHHCCCEEEEEE
TGVIGMEFASIYSQFGTEVTVVGNKLLKTEDGEIQKRLKSILKSDTLKFVTGVYAKKIVE
CCHHHHHHHHHHHHHCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EDGRLKIISQKVGKDKFEETYADYVLVASGRSSNTDNLGLENTDIKTENNAIVVDENLQT
CCCCEEEEHHHCCCHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCC
NVEGIYSIGDCVYKNTQLAHVASNQGKNLVREFSGKDRNINMDIVPAVVFTVPEIASVGL
CCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCC
TEEKAKEQNIDYVTSKFMYQANGKALSLNATEGFVKIVATKDLSKILGCHIIGHDASTII
CHHHHHHCCCHHHHHHHEEECCCCEEEEECCCCEEEEEEHHHHHHHHCEEEECCCHHHHH
HFAAIAMNNNVGVEGLSAMIYAHPTISEVFMDSVEQLEGLSINTPNPNK
HEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]