Click here to switch to the map view.

The map label for this gene is thiM

Identifier: 169824074

GI number: 169824074

Start: 430970

End: 431776

Strand: Direct

Name: thiM

Synonym: FMG_0377

Alternate gene names: 169824074

Gene position: 430970-431776 (Clockwise)

Preceding gene: 169824073

Following gene: 169824075

Centisome position: 23.98

GC content: 32.84

Gene sequence:

>807_bases
ATGGATAATTTAATACAAAAAATGTCAGAGATTTACTCTGATTTGCACAACAGTAATAATCTGATCCATTGCCTTACAAA
TGCAATATCTATCAATGATATGGCAAACGCTGTGCTAAGTATAGGCCAAAGCCCATTCATGGCAGATAATCCTAGGGAAG
TGGAAGATGTTACGAGAAAATCTGATTCTCTGTTGGTAAATTTAGGAAACATAACGGATTATAGGATAGAATCAATCAAA
AAATCTGTGCGAATTGCAAATGAAAACAACATTCCGATTACTTTGGATTTGGTTGGAGTGAGTGCATCAAAATTAAGGTT
GGATTTGACTTTAGACATTTTGAAAAATCATACAATAACTTGTATTAAAGGAAATTACAGCGAGATAAAATCACTGTTTA
TTAAAGATTTAAGTACAAAAGGTGTGGATTCCCAAAAACTGGAAGTAGACGATGTTATTAATTGTTGTAAAAATTTACAC
GAAAAATACGATTCGATAATTGTAGCAACAGGGCAGACCGATATTGTGTGTGCAAATGAGATTTATCTACTAAACAATGG
AGATGATAGACTTTCGAAAATCACTGCTACAGGTTGTGTTGTGGGAAGTTTAATTGCTTGTACACTTAGCGTATCTCAAT
CGATAAAAGCATGTGTTCTAGCAATTAGCATGATGAATATTAGCTGTGAAATGGTGGATAAAAGTGTTGGACTTAGTTCG
TTTAAATTAGGTTTGTACGATAATTTATCCAGAATAAAATGGGAACAAATAAAGGAGAATATAGATGCGAAAAAAGTTGA
ATCTTAG

Upstream 100 bases:

>100_bases
TCATTTGTGCTGGCGACTTTAATGGGAGCGACTGTAGCATTCTTATTTTTAAAGGCTATGCAAAAAAATAAAATGCTAGA
TAAATTAAAGGAAAGTATCA

Downstream 100 bases:

>100_bases
CTTGTACTTGGTTACTGATAGGACAAATGTAGCTTGTGAAGAAAAATTTCTCACAAAAATTGAAGAATCACTTAAAGGTG
GAGTAACACTGGTACAACTA

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MDNLIQKMSEIYSDLHNSNNLIHCLTNAISINDMANAVLSIGQSPFMADNPREVEDVTRKSDSLLVNLGNITDYRIESIK
KSVRIANENNIPITLDLVGVSASKLRLDLTLDILKNHTITCIKGNYSEIKSLFIKDLSTKGVDSQKLEVDDVINCCKNLH
EKYDSIIVATGQTDIVCANEIYLLNNGDDRLSKITATGCVVGSLIACTLSVSQSIKACVLAISMMNISCEMVDKSVGLSS
FKLGLYDNLSRIKWEQIKENIDAKKVES

Sequences:

>Translated_268_residues
MDNLIQKMSEIYSDLHNSNNLIHCLTNAISINDMANAVLSIGQSPFMADNPREVEDVTRKSDSLLVNLGNITDYRIESIK
KSVRIANENNIPITLDLVGVSASKLRLDLTLDILKNHTITCIKGNYSEIKSLFIKDLSTKGVDSQKLEVDDVINCCKNLH
EKYDSIIVATGQTDIVCANEIYLLNNGDDRLSKITATGCVVGSLIACTLSVSQSIKACVLAISMMNISCEMVDKSVGLSS
FKLGLYDNLSRIKWEQIKENIDAKKVES
>Mature_268_residues
MDNLIQKMSEIYSDLHNSNNLIHCLTNAISINDMANAVLSIGQSPFMADNPREVEDVTRKSDSLLVNLGNITDYRIESIK
KSVRIANENNIPITLDLVGVSASKLRLDLTLDILKNHTITCIKGNYSEIKSLFIKDLSTKGVDSQKLEVDDVINCCKNLH
EKYDSIIVATGQTDIVCANEIYLLNNGDDRLSKITATGCVVGSLIACTLSVSQSIKACVLAISMMNISCEMVDKSVGLSS
FKLGLYDNLSRIKWEQIKENIDAKKVES

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=248, Percent_Identity=27.8225806451613, Blast_Score=98, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6325042, Length=283, Percent_Identity=30.7420494699647, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_FINM2 (B0S0L6)

Other databases:

- EMBL:   AP008971
- RefSeq:   YP_001691685.1
- ProteinModelPortal:   B0S0L6
- SMR:   B0S0L6
- GeneID:   6019048
- GenomeReviews:   AP008971_GR
- KEGG:   fma:FMG_0377
- HOGENOM:   HBG351126
- OMA:   MGICGEL
- BioCyc:   FMAG334413:FMG_0377-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 29536; Mature: 29536

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 47-47 BINDING 123-123 BINDING 170-170 BINDING 196-196

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLIQKMSEIYSDLHNSNNLIHCLTNAISINDMANAVLSIGQSPFMADNPREVEDVTRK
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SDSLLVNLGNITDYRIESIKKSVRIANENNIPITLDLVGVSASKLRLDLTLDILKNHTIT
CCCEEEEECCCCHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCEEEEEEEEHHCCCEEE
CIKGNYSEIKSLFIKDLSTKGVDSQKLEVDDVINCCKNLHEKYDSIIVATGQTDIVCANE
EEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEEECE
IYLLNNGDDRLSKITATGCVVGSLIACTLSVSQSIKACVLAISMMNISCEMVDKSVGLSS
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKLGLYDNLSRIKWEQIKENIDAKKVES
EEECHHHCHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MDNLIQKMSEIYSDLHNSNNLIHCLTNAISINDMANAVLSIGQSPFMADNPREVEDVTRK
CCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCC
SDSLLVNLGNITDYRIESIKKSVRIANENNIPITLDLVGVSASKLRLDLTLDILKNHTIT
CCCEEEEECCCCHHHHHHHHHHHEECCCCCCCEEEEEECCCCCCEEEEEEEEHHCCCEEE
CIKGNYSEIKSLFIKDLSTKGVDSQKLEVDDVINCCKNLHEKYDSIIVATGQTDIVCANE
EEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCEEEECE
IYLLNNGDDRLSKITATGCVVGSLIACTLSVSQSIKACVLAISMMNISCEMVDKSVGLSS
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FKLGLYDNLSRIKWEQIKENIDAKKVES
EEECHHHCHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA