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The map label for this gene is thiE2 [H]

Identifier: 169824075

GI number: 169824075

Start: 431754

End: 432398

Strand: Direct

Name: thiE2 [H]

Synonym: FMG_0378

Alternate gene names: 169824075

Gene position: 431754-432398 (Clockwise)

Preceding gene: 169824074

Following gene: 169824076

Centisome position: 24.02

GC content: 36.43

Gene sequence:

>645_bases
ATGCGAAAAAAGTTGAATCTTAGCTTGTACTTGGTTACTGATAGGACAAATGTAGCTTGTGAAGAAAAATTTCTCACAAA
AATTGAAGAATCACTTAAAGGTGGAGTAACACTGGTACAACTAAGAGAGAAAAATATCTCCACAAGAGAATACATAGACT
TGGCGAAAAAAGTGAAGATAATTTGTGATAAATTCGATGTGCCGCTTTTAATTGACGACAGAATTGACGTTTGCTTGGCA
AGCGAGTGTGCAGGAGTTCATCTTGGAGATGAGGACATGGAAATAAAAGATGCGCGTAAGATTTTGGGGGATAATTACAT
TATTGGTGCAACTGCAAAAAGTGTTGAGAGAGCTGTCCAATGTGAAAAAGAAGGCGCAGATTATCTTGGAGTTGGAGCAA
TTTATCCGACAAAAACCCACGTTAAAACGAAAATAACATCGGTTGATACCCTAAGAGATATCAACAATAGCATAAACATA
AAAACGGTCGCTATTGGCGGTTTAAATGAGGATAACATGGATGTATTAAAGAACAGTGGTGCAAGTGGAATAGCTGTCGT
AAGAGCATTGATGAATGACGATAATCCACAAGAAAAAGCACACAGATTATTAGAGAAATCAAAACAAATTTTAGAATTGA
GGTAA

Upstream 100 bases:

>100_bases
TGTGAAATGGTGGATAAAAGTGTTGGACTTAGTTCGTTTAAATTAGGTTTGTACGATAATTTATCCAGAATAAAATGGGA
ACAAATAAAGGAGAATATAG

Downstream 100 bases:

>100_bases
TTATGAAAAAGAAATTTAAATTAGTATTGTTAGTTTTTGTGTTGATGTTAGTCATGGTATCTTGCAAAAAAGCAGATCCA
ATAACACTTCCAAATGCCAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase 2; TMP-PPase 2; Thiamine-phosphate synthase 2 [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MRKKLNLSLYLVTDRTNVACEEKFLTKIEESLKGGVTLVQLREKNISTREYIDLAKKVKIICDKFDVPLLIDDRIDVCLA
SECAGVHLGDEDMEIKDARKILGDNYIIGATAKSVERAVQCEKEGADYLGVGAIYPTKTHVKTKITSVDTLRDINNSINI
KTVAIGGLNEDNMDVLKNSGASGIAVVRALMNDDNPQEKAHRLLEKSKQILELR

Sequences:

>Translated_214_residues
MRKKLNLSLYLVTDRTNVACEEKFLTKIEESLKGGVTLVQLREKNISTREYIDLAKKVKIICDKFDVPLLIDDRIDVCLA
SECAGVHLGDEDMEIKDARKILGDNYIIGATAKSVERAVQCEKEGADYLGVGAIYPTKTHVKTKITSVDTLRDINNSINI
KTVAIGGLNEDNMDVLKNSGASGIAVVRALMNDDNPQEKAHRLLEKSKQILELR
>Mature_214_residues
MRKKLNLSLYLVTDRTNVACEEKFLTKIEESLKGGVTLVQLREKNISTREYIDLAKKVKIICDKFDVPLLIDDRIDVCLA
SECAGVHLGDEDMEIKDARKILGDNYIIGATAKSVERAVQCEKEGADYLGVGAIYPTKTHVKTKITSVDTLRDINNSINI
KTVAIGGLNEDNMDVLKNSGASGIAVVRALMNDDNPQEKAHRLLEKSKQILELR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=184, Percent_Identity=39.1304347826087, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23759; Mature: 23759

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKLNLSLYLVTDRTNVACEEKFLTKIEESLKGGVTLVQLREKNISTREYIDLAKKVKI
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
ICDKFDVPLLIDDRIDVCLASECAGVHLGDEDMEIKDARKILGDNYIIGATAKSVERAVQ
HHCCCCCCEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
CEKEGADYLGVGAIYPTKTHVKTKITSVDTLRDINNSINIKTVAIGGLNEDNMDVLKNSG
HHHCCCCEEEECEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCC
ASGIAVVRALMNDDNPQEKAHRLLEKSKQILELR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKKLNLSLYLVTDRTNVACEEKFLTKIEESLKGGVTLVQLREKNISTREYIDLAKKVKI
CCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHH
ICDKFDVPLLIDDRIDVCLASECAGVHLGDEDMEIKDARKILGDNYIIGATAKSVERAVQ
HHCCCCCCEEECCCCCEEECCCCCCCEECCCCCHHHHHHHHHCCCEEEECCHHHHHHHHH
CEKEGADYLGVGAIYPTKTHVKTKITSVDTLRDINNSINIKTVAIGGLNEDNMDVLKNSG
HHHCCCCEEEECEECCCCCCCEEEHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHCCC
ASGIAVVRALMNDDNPQEKAHRLLEKSKQILELR
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]