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The map label for this gene is radA [H]

Identifier: 169824033

GI number: 169824033

Start: 390784

End: 392145

Strand: Direct

Name: radA [H]

Synonym: FMG_0336

Alternate gene names: 169824033

Gene position: 390784-392145 (Clockwise)

Preceding gene: 169824032

Following gene: 169824036

Centisome position: 21.74

GC content: 37.0

Gene sequence:

>1362_bases
ATGAAAAAGAAAACAAAATACGTGTGCAAAAATTGTGGCTATGAACAGGCCATGTGGATAGGAAAATGCCCCAGTTGTAA
CAGCTGGGGTTCTATGCAAGAACAAGAAATCGTAGATGAAAAATCAAATACTACAACAACAAGTTCGACTAAGTCTTCAA
AAGCAGTTAAATTAGTCGATGTAAAGATAGATTCGTCGGAAAGAATTCAATCGTCGATTACTGAATTAAACAGAGTTTTT
GGCGGAGGACTTGTGCGTGATTCCGTGTCAATTCTAACTGCAAGACCAGGAGCAGGGAAATCGACTTTGCTGTTGGAACT
TGCAAATGATTTGGCTTCTAAAAACATAAAAGTTCTCTACATTTCTGGAGAAGAATCCACATCTCAAATCAAACAAAGAG
CGATGAGAACTATGGAGAAAATCCCGGAAAATATTTGGTTGATTTCAACAACTAGTATGGACAATGCGTTGGATTCAATC
GATAAAATAAAACCAGATGTGATTTTCTTGGATAGTATTCAAACATTCCAACTTCGTGAATACACATCACGCCCAGGATC
TCCTGTGCAAACTGATGAATGTGCGAATAAAATCGTAGAAATCTGCAAAAATCCCGAAAAGCCAACTCTAGCGATTATGG
TTGGTCACATGACAAAATCAGACGAAATGGCAGGGCTTAGAACGCTTGAACATTTGGTGGATACTGTGCTTTATTTGGAA
GGTGAAAGTGACGATCCTTTGAGATTGTTGACAACTACGAAAAATAGATTCGGTCGAACTGGTGAGATTGGACTGTTTAA
TATGCAAGAAGATGGAATAAAAGAAATCACCGATGCGTCTGATTATTTCGTCACGAAAAGAAAAAAAGATGTAAATGGTG
CAGCACTTTCTGTAATAAAAGAAGGTTCACGAATTTTGGTTGTGGAAGTGGAAGCATTGGTGTCCAAATCATTCACACCG
TATCCGCAAAGAATAGGTGACAGTCTTCGCAAAGACCAATTAAACACTTTGATTTCTATCTTGGAAGAACGCGCTGGAAT
GAATATGTACGACAAAAATGTCATCATCAAAACCACAGGTGGAATCAAAATTCAAGAACAGTCTGTGAATTTGTCCATAA
TAATGGCGATTGCATCATCACTTATGAACAAACCAATTCCATCAGATGTAGTGTTCATCGGAGAAGTTGGATTGACAGGA
GAAATCAAAAGAGTCCCACAAATAGAAACCAGATTAAAAGAAATCGAAAGATTGGGATACAAAAAAGTTTATATCTCACA
AAACACGAAACTAACACAAAAATACGATCTCGAAATCAAACAATTTGATAGCATAAGATCGGTAATATCAGATATATTTT
AA

Upstream 100 bases:

>100_bases
GGAGATATCGACAATAACAGAAAAACGACTATAACTGTGGTTAACGACAAGGTTAGCTTCAGAAGATATGTAAGACGCAA
AAAGACTGTGAAACAATAAT

Downstream 100 bases:

>100_bases
AAGGCACTCAAAAATTGAGCGCCTTTTTCTAATTCTTTCCTAATAGTAATTTGTTGTTATCAATGTAGGCTTTTATTGCC
GACAAATCATTTTTCTCATA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MKKKTKYVCKNCGYEQAMWIGKCPSCNSWGSMQEQEIVDEKSNTTTTSSTKSSKAVKLVDVKIDSSERIQSSITELNRVF
GGGLVRDSVSILTARPGAGKSTLLLELANDLASKNIKVLYISGEESTSQIKQRAMRTMEKIPENIWLISTTSMDNALDSI
DKIKPDVIFLDSIQTFQLREYTSRPGSPVQTDECANKIVEICKNPEKPTLAIMVGHMTKSDEMAGLRTLEHLVDTVLYLE
GESDDPLRLLTTTKNRFGRTGEIGLFNMQEDGIKEITDASDYFVTKRKKDVNGAALSVIKEGSRILVVEVEALVSKSFTP
YPQRIGDSLRKDQLNTLISILEERAGMNMYDKNVIIKTTGGIKIQEQSVNLSIIMAIASSLMNKPIPSDVVFIGEVGLTG
EIKRVPQIETRLKEIERLGYKKVYISQNTKLTQKYDLEIKQFDSIRSVISDIF

Sequences:

>Translated_453_residues
MKKKTKYVCKNCGYEQAMWIGKCPSCNSWGSMQEQEIVDEKSNTTTTSSTKSSKAVKLVDVKIDSSERIQSSITELNRVF
GGGLVRDSVSILTARPGAGKSTLLLELANDLASKNIKVLYISGEESTSQIKQRAMRTMEKIPENIWLISTTSMDNALDSI
DKIKPDVIFLDSIQTFQLREYTSRPGSPVQTDECANKIVEICKNPEKPTLAIMVGHMTKSDEMAGLRTLEHLVDTVLYLE
GESDDPLRLLTTTKNRFGRTGEIGLFNMQEDGIKEITDASDYFVTKRKKDVNGAALSVIKEGSRILVVEVEALVSKSFTP
YPQRIGDSLRKDQLNTLISILEERAGMNMYDKNVIIKTTGGIKIQEQSVNLSIIMAIASSLMNKPIPSDVVFIGEVGLTG
EIKRVPQIETRLKEIERLGYKKVYISQNTKLTQKYDLEIKQFDSIRSVISDIF
>Mature_453_residues
MKKKTKYVCKNCGYEQAMWIGKCPSCNSWGSMQEQEIVDEKSNTTTTSSTKSSKAVKLVDVKIDSSERIQSSITELNRVF
GGGLVRDSVSILTARPGAGKSTLLLELANDLASKNIKVLYISGEESTSQIKQRAMRTMEKIPENIWLISTTSMDNALDSI
DKIKPDVIFLDSIQTFQLREYTSRPGSPVQTDECANKIVEICKNPEKPTLAIMVGHMTKSDEMAGLRTLEHLVDTVLYLE
GESDDPLRLLTTTKNRFGRTGEIGLFNMQEDGIKEITDASDYFVTKRKKDVNGAALSVIKEGSRILVVEVEALVSKSFTP
YPQRIGDSLRKDQLNTLISILEERAGMNMYDKNVIIKTTGGIKIQEQSVNLSIIMAIASSLMNKPIPSDVVFIGEVGLTG
EIKRVPQIETRLKEIERLGYKKVYISQNTKLTQKYDLEIKQFDSIRSVISDIF

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=429, Percent_Identity=34.032634032634, Blast_Score=296, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50580; Mature: 50580

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKTKYVCKNCGYEQAMWIGKCPSCNSWGSMQEQEIVDEKSNTTTTSSTKSSKAVKLVD
CCCCHHHHHHCCCCHHHEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEE
VKIDSSERIQSSITELNRVFGGGLVRDSVSILTARPGAGKSTLLLELANDLASKNIKVLY
EEECCHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEE
ISGEESTSQIKQRAMRTMEKIPENIWLISTTSMDNALDSIDKIKPDVIFLDSIQTFQLRE
EECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHH
YTSRPGSPVQTDECANKIVEICKNPEKPTLAIMVGHMTKSDEMAGLRTLEHLVDTVLYLE
HHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEE
GESDDPLRLLTTTKNRFGRTGEIGLFNMQEDGIKEITDASDYFVTKRKKDVNGAALSVIK
CCCCCCEEEEEECHHHCCCCCCEEEEECCCCCHHHHHCCCHHEEECCCCCCCHHHHHHHH
EGSRILVVEVEALVSKSFTPYPQRIGDSLRKDQLNTLISILEERAGMNMYDKNVIIKTTG
CCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECC
GIKIQEQSVNLSIIMAIASSLMNKPIPSDVVFIGEVGLTGEIKRVPQIETRLKEIERLGY
CEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
KKVYISQNTKLTQKYDLEIKQFDSIRSVISDIF
EEEEEECCCCCCCEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKKTKYVCKNCGYEQAMWIGKCPSCNSWGSMQEQEIVDEKSNTTTTSSTKSSKAVKLVD
CCCCHHHHHHCCCCHHHEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCEEEEE
VKIDSSERIQSSITELNRVFGGGLVRDSVSILTARPGAGKSTLLLELANDLASKNIKVLY
EEECCHHHHHHHHHHHHHHHCCCHHHCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEE
ISGEESTSQIKQRAMRTMEKIPENIWLISTTSMDNALDSIDKIKPDVIFLDSIQTFQLRE
EECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCEEEECCCCHHHHHH
YTSRPGSPVQTDECANKIVEICKNPEKPTLAIMVGHMTKSDEMAGLRTLEHLVDTVLYLE
HHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHEEEE
GESDDPLRLLTTTKNRFGRTGEIGLFNMQEDGIKEITDASDYFVTKRKKDVNGAALSVIK
CCCCCCEEEEEECHHHCCCCCCEEEEECCCCCHHHHHCCCHHEEECCCCCCCHHHHHHHH
EGSRILVVEVEALVSKSFTPYPQRIGDSLRKDQLNTLISILEERAGMNMYDKNVIIKTTG
CCCEEEEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECC
GIKIQEQSVNLSIIMAIASSLMNKPIPSDVVFIGEVGLTGEIKRVPQIETRLKEIERLGY
CEEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCC
KKVYISQNTKLTQKYDLEIKQFDSIRSVISDIF
EEEEEECCCCCCCEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]