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The map label for this gene is waaA [H]

Identifier: 167626118

GI number: 167626118

Start: 5117091

End: 5118356

Strand: Reverse

Name: waaA [H]

Synonym: Shal_4213

Alternate gene names: 167626118

Gene position: 5118356-5117091 (Counterclockwise)

Preceding gene: 167626124

Following gene: 167626116

Centisome position: 97.92

GC content: 50.32

Gene sequence:

>1266_bases
ATGAATCGTAGCCTCTATTCTTTAGCTTTATATTTAATTAGCCCTCTATTGCTGGTGTATCTTGCTGTGCGCGCCTTTAA
AAGCAAAGACTATCGTGGTCGCTGGAATGAGCGATTCGGCCTTAAGTCGCTAAAGCAAACCGATCTCTTGGTACATTCGG
TATCAATGGGGGAGACCTTAGCGGCTATTCCTCTAATAAAGCAGATCCAACTGCAACACCCGCAGTTAAGCATTACCATC
ACCACAACCAGCCCAACGGGCTCTGCCGAGGTTGCACGCGCCTTTGGGAATAGTGTGCAACACTGCTATTTACCTTTCGA
TCTCGCTTGGTGCGCCAAGCGTTTTGTAAAGCAAGTCGCGCCTAAGTGCTGCATCATCATGGAAACCGAGCTTTGGCCTA
ATCTAATCCATTACCTTAAACAAGCGGGGACGCAGGTCTTATTAGCTAATGCGCGTTTGTCGCAAAAGTCGGCCGACTCC
TACCGTAAGCATCACAAGTTAACCACGCCAATGCTTAAGCTGCTCGATGGTATTGCGGCGCAGTCACAGCAGGCTGCAGA
GCGCTTTATCGCTTTGGGTGTTGCGCCGGAACGAGTCACGGTTTGTGGCAGCTTAAAGTTCGATATCAATATCGATAAGC
AGCGCATCGATACAGCAATAAAACTCAGAGCACAGTGGCTGGCAACGGATAAACCGGTTTGGGTGGCAGGCTCGGTGCAT
CCGGGAGAGTTTGATGCGTTAATTATGGCGCATCAACAACTGCTAGCGAAATACCCCAATGCCATGATGGTGATGGTGCC
GCGCCACCCTGAGCAATTTGACGCAGCGGCGCAAGCAATTAAGTCGGCAGGGTTAACCCTTATTCGACGTAGCATGGCTA
AGCCTGTATTGCCTGAAACCCAGGTTGTATTGGGTAACACCATGGGCGAGTTGTTGACCTTTTATGGCGCTGCCGATCAA
GCCTTTGTCGGGGGCTCAATAATCGTCCATGGCGGCCATAATCCACTAGAGCCTGCGGCTATGGGGTTACCTGTGATGAT
GGGGCCACATTACCGTGATTTTAATGAAATCACTCAGCTACTTGAGTCTGCGGGGGCGCTAAAAGTCGTTGATTCAGCAG
AGGCTCTGGCAGAACGCTTGATCTATTTATTCGATGATAAGGCGGCTTATAACCAAGCGAGCGCAGCTGCAAGGGCTGTG
GTTGAGCAGAACCGGGGTTCGCTCACAAAGCAGTTGGAGGTAGTGGAGTCGTTTATTAACGGTTAG

Upstream 100 bases:

>100_bases
ATCCTGTTTGAATTACACATGTGTAATTACGTTAAGGTTTAACTATCATGTTAGCATTAACGCAATTGTTCAAGTAAAAC
GCAAGAAAGTAGCAAATTTC

Downstream 100 bases:

>100_bases
CGCGAATACCGTTTAGATGGAGCATAAAAAAGGTCTGCAGCTTGTGTAGACCTTTTTTAGTTTGGCTGTGGCTTATCGAG
AGATTAAGTTGACAGGAAAG

Product: 3-deoxy-D-manno-octulosonic-acid transferase domain-containing protein

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNRSLYSLALYLISPLLLVYLAVRAFKSKDYRGRWNERFGLKSLKQTDLLVHSVSMGETLAAIPLIKQIQLQHPQLSITI
TTTSPTGSAEVARAFGNSVQHCYLPFDLAWCAKRFVKQVAPKCCIIMETELWPNLIHYLKQAGTQVLLANARLSQKSADS
YRKHHKLTTPMLKLLDGIAAQSQQAAERFIALGVAPERVTVCGSLKFDINIDKQRIDTAIKLRAQWLATDKPVWVAGSVH
PGEFDALIMAHQQLLAKYPNAMMVMVPRHPEQFDAAAQAIKSAGLTLIRRSMAKPVLPETQVVLGNTMGELLTFYGAADQ
AFVGGSIIVHGGHNPLEPAAMGLPVMMGPHYRDFNEITQLLESAGALKVVDSAEALAERLIYLFDDKAAYNQASAAARAV
VEQNRGSLTKQLEVVESFING

Sequences:

>Translated_421_residues
MNRSLYSLALYLISPLLLVYLAVRAFKSKDYRGRWNERFGLKSLKQTDLLVHSVSMGETLAAIPLIKQIQLQHPQLSITI
TTTSPTGSAEVARAFGNSVQHCYLPFDLAWCAKRFVKQVAPKCCIIMETELWPNLIHYLKQAGTQVLLANARLSQKSADS
YRKHHKLTTPMLKLLDGIAAQSQQAAERFIALGVAPERVTVCGSLKFDINIDKQRIDTAIKLRAQWLATDKPVWVAGSVH
PGEFDALIMAHQQLLAKYPNAMMVMVPRHPEQFDAAAQAIKSAGLTLIRRSMAKPVLPETQVVLGNTMGELLTFYGAADQ
AFVGGSIIVHGGHNPLEPAAMGLPVMMGPHYRDFNEITQLLESAGALKVVDSAEALAERLIYLFDDKAAYNQASAAARAV
VEQNRGSLTKQLEVVESFING
>Mature_421_residues
MNRSLYSLALYLISPLLLVYLAVRAFKSKDYRGRWNERFGLKSLKQTDLLVHSVSMGETLAAIPLIKQIQLQHPQLSITI
TTTSPTGSAEVARAFGNSVQHCYLPFDLAWCAKRFVKQVAPKCCIIMETELWPNLIHYLKQAGTQVLLANARLSQKSADS
YRKHHKLTTPMLKLLDGIAAQSQQAAERFIALGVAPERVTVCGSLKFDINIDKQRIDTAIKLRAQWLATDKPVWVAGSVH
PGEFDALIMAHQQLLAKYPNAMMVMVPRHPEQFDAAAQAIKSAGLTLIRRSMAKPVLPETQVVLGNTMGELLTFYGAADQ
AFVGGSIIVHGGHNPLEPAAMGLPVMMGPHYRDFNEITQLLESAGALKVVDSAEALAERLIYLFDDKAAYNQASAAARAV
VEQNRGSLTKQLEVVESFING

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=417, Percent_Identity=47.0023980815348, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46343; Mature: 46343

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSLYSLALYLISPLLLVYLAVRAFKSKDYRGRWNERFGLKSLKQTDLLVHSVSMGETL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHHHHEECCCCCHH
AAIPLIKQIQLQHPQLSITITTTSPTGSAEVARAFGNSVQHCYLPFDLAWCAKRFVKQVA
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCC
PKCCIIMETELWPNLIHYLKQAGTQVLLANARLSQKSADSYRKHHKLTTPMLKLLDGIAA
CCEEEEEEHHHHHHHHHHHHHCCCEEEEECCHHCHHHHHHHHHHHCHHHHHHHHHHHHHH
QSQQAAERFIALGVAPERVTVCGSLKFDINIDKQRIDTAIKLRAQWLATDKPVWVAGSVH
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCC
PGEFDALIMAHQQLLAKYPNAMMVMVPRHPEQFDAAAQAIKSAGLTLIRRSMAKPVLPET
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVVLGNTMGELLTFYGAADQAFVGGSIIVHGGHNPLEPAAMGLPVMMGPHYRDFNEITQL
HHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCHHHCCCCEEECCCCCCHHHHHHH
LESAGALKVVDSAEALAERLIYLFDDKAAYNQASAAARAVVEQNRGSLTKQLEVVESFIN
HHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MNRSLYSLALYLISPLLLVYLAVRAFKSKDYRGRWNERFGLKSLKQTDLLVHSVSMGETL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCHHHHHHHHHHHEECCCCCHH
AAIPLIKQIQLQHPQLSITITTTSPTGSAEVARAFGNSVQHCYLPFDLAWCAKRFVKQVA
HHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHCC
PKCCIIMETELWPNLIHYLKQAGTQVLLANARLSQKSADSYRKHHKLTTPMLKLLDGIAA
CCEEEEEEHHHHHHHHHHHHHCCCEEEEECCHHCHHHHHHHHHHHCHHHHHHHHHHHHHH
QSQQAAERFIALGVAPERVTVCGSLKFDINIDKQRIDTAIKLRAQWLATDKPVWVAGSVH
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCEEEECCCC
PGEFDALIMAHQQLLAKYPNAMMVMVPRHPEQFDAAAQAIKSAGLTLIRRSMAKPVLPET
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QVVLGNTMGELLTFYGAADQAFVGGSIIVHGGHNPLEPAAMGLPVMMGPHYRDFNEITQL
HHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCCHHHCCCCEEECCCCCCHHHHHHH
LESAGALKVVDSAEALAERLIYLFDDKAAYNQASAAARAVVEQNRGSLTKQLEVVESFIN
HHCCCCEEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]