Click here to switch to the map view.

The map label for this gene is iaaA [H]

Identifier: 167623775

GI number: 167623775

Start: 2205385

End: 2206458

Strand: Direct

Name: iaaA [H]

Synonym: Shal_1845

Alternate gene names: 167623775

Gene position: 2205385-2206458 (Clockwise)

Preceding gene: 167623772

Following gene: 167623776

Centisome position: 42.19

GC content: 46.0

Gene sequence:

>1074_bases
ATGCTAAGTCGTACATCCTTGCTTATATTATCAACAGTTATGTCTGCAAGTAGCATGGCCAGTGATAGGCCTTTTTCAAT
TGCGATTCATGGTGGCGCAGGCACCATTTCCAAGGCAAATTTAACCGCAGAACAGCAACAAGCTTATCGCGCGAAATTAC
AGCAAGCGGTTGATGCGGGTTACGATATTCTAGCCAAAGGGGGAGATAGCTTATCGGCCGTTCGAGCATCGATAAATATC
CTAGAAAACAGCCCGCTATTTAATGCGGGCATTGGCGCGGTATATACCTATGATGGTGGTCATGAATTGGACGCATCGAT
CATGGATGGAAAAACCATGAATGCTGGCGCCGTTGCTGGGGTAAAACATATTAAAAATCCGATCGACTTAGCCTTCACCG
TGATGGAAAAGTCACCCCATGTGATGTTATCGGGTCAAGGTGCTGAAGAGTTTGCTCTCACTCAGGGTTTTCAGTTAGTT
CCTGTAAACACATTCGATACCGATAGTCGTTATCAGCAATTACAAAGCGCCAAAGAAAAAATCCAAAAAGCGGAGTCAAA
CTCTGATTACCAAGCGAGCACTGGGCATAGTACTGGCGTTAATACTCACCATAATTACCAACAAAGTGCCCTCGATTACA
GCGAGTTTGACTATAAATTTGGCACCGTGGGCGCCGTTGCTCTAGATCAAAATGGTAATCTAGCAGCAGGAACCTCAACT
GGTGGTATGACGGCTAAGCGTTTTGGCCGCATTGGTGACTCACCTGTTATTGGAGCTGGTACCTATGCTGAAAATGGTGT
GTGCGCCGTATCGGCAACAGGTCATGGCGAGTATTTTATTCGCTATCACGTTGCGGGAGATATCTGCGCTAAGGTCAAAT
ATCAGAAGAAATCGATTATTCAAGCGGCCGATGAGGTGATTAATCAGCGTCTAATCACTGCTGGTGGTACCGGTGGTGTT
GTTGCTATCGATCAGCGCGGTAATATTGCAACACCATTTAACACCGAAGGTATGTACCGTGCGACGCGTAAGAATAACGA
GCCTGCACAGGTGATGATCTGGTCTGATTTATAA

Upstream 100 bases:

>100_bases
AGAATCGTTAGCTCAAGCAAGTTGCTGATTGTAATTATGGTCGAAAATGGTTACAAAGAGAGCAAATAAATACAATAGGA
TACAGTTTGTGAAGTTCAAA

Downstream 100 bases:

>100_bases
TCAAAGATGAAGCGCACTAAGATCAACTTGCAGAGCTTGGCGCGCTTATACTCGGTTAACATATCGCCTTAATGGTTATC
GATTTATTCTCTATTAAGGC

Product: peptidase T2 asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MLSRTSLLILSTVMSASSMASDRPFSIAIHGGAGTISKANLTAEQQQAYRAKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAVAGVKHIKNPIDLAFTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNTFDTDSRYQQLQSAKEKIQKAESNSDYQASTGHSTGVNTHHNYQQSALDYSEFDYKFGTVGAVALDQNGNLAAGTST
GGMTAKRFGRIGDSPVIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGGTGGV
VAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL

Sequences:

>Translated_357_residues
MLSRTSLLILSTVMSASSMASDRPFSIAIHGGAGTISKANLTAEQQQAYRAKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAVAGVKHIKNPIDLAFTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNTFDTDSRYQQLQSAKEKIQKAESNSDYQASTGHSTGVNTHHNYQQSALDYSEFDYKFGTVGAVALDQNGNLAAGTST
GGMTAKRFGRIGDSPVIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGGTGGV
VAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL
>Mature_357_residues
MLSRTSLLILSTVMSASSMASDRPFSIAIHGGAGTISKANLTAEQQQAYRAKLQQAVDAGYDILAKGGDSLSAVRASINI
LENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAVAGVKHIKNPIDLAFTVMEKSPHVMLSGQGAEEFALTQGFQLV
PVNTFDTDSRYQQLQSAKEKIQKAESNSDYQASTGHSTGVNTHHNYQQSALDYSEFDYKFGTVGAVALDQNGNLAAGTST
GGMTAKRFGRIGDSPVIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSIIQAADEVINQRLITAGGTGGV
VAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=324, Percent_Identity=37.037037037037, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI145275200, Length=324, Percent_Identity=37.037037037037, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI98991769, Length=330, Percent_Identity=29.6969696969697, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI285002251, Length=254, Percent_Identity=31.8897637795276, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI285002253, Length=254, Percent_Identity=30.3149606299213, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787050, Length=315, Percent_Identity=48.8888888888889, Blast_Score=291, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI17569239, Length=248, Percent_Identity=28.2258064516129, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI18921183, Length=321, Percent_Identity=33.6448598130841, Blast_Score=168, Evalue=4e-42,
Organism=Drosophila melanogaster, GI21357893, Length=277, Percent_Identity=33.9350180505415, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24652135, Length=235, Percent_Identity=34.8936170212766, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI221330102, Length=235, Percent_Identity=34.8936170212766, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24655553, Length=219, Percent_Identity=31.9634703196347, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24657358, Length=247, Percent_Identity=28.7449392712551, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 37837; Mature: 37837

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRTSLLILSTVMSASSMASDRPFSIAIHGGAGTISKANLTAEQQQAYRAKLQQAVDAG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCC
YDILAKGGDSLSAVRASINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAVAG
CEEEECCCCHHHHHHHEEEEEECCCCEECCCCEEEEECCCCEECCEEECCCCCCCCHHHH
VKHIKNPIDLAFTVMEKSPHVMLSGQGAEEFALTQGFQLVPVNTFDTDSRYQQLQSAKEK
HHHHCCCHHEEEEEECCCCEEEEECCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
IQKAESNSDYQASTGHSTGVNTHHNYQQSALDYSEFDYKFGTVGAVALDQNGNLAAGTST
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHCCEECCCEEEEEECCCCCEEECCCC
GGMTAKRFGRIGDSPVIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSII
CCCCHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHH
QAADEVINQRLITAGGTGGVVAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL
HHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHCCCCCCCEEEEEECC
>Mature Secondary Structure
MLSRTSLLILSTVMSASSMASDRPFSIAIHGGAGTISKANLTAEQQQAYRAKLQQAVDAG
CCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCCHHHHHHHHHHHHHHHHCC
YDILAKGGDSLSAVRASINILENSPLFNAGIGAVYTYDGGHELDASIMDGKTMNAGAVAG
CEEEECCCCHHHHHHHEEEEEECCCCEECCCCEEEEECCCCEECCEEECCCCCCCCHHHH
VKHIKNPIDLAFTVMEKSPHVMLSGQGAEEFALTQGFQLVPVNTFDTDSRYQQLQSAKEK
HHHHCCCHHEEEEEECCCCEEEEECCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHH
IQKAESNSDYQASTGHSTGVNTHHNYQQSALDYSEFDYKFGTVGAVALDQNGNLAAGTST
HHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHCCEECCCEEEEEECCCCCEEECCCC
GGMTAKRFGRIGDSPVIGAGTYAENGVCAVSATGHGEYFIRYHVAGDICAKVKYQKKSII
CCCCHHHHCCCCCCCEEECCCCCCCCEEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHH
QAADEVINQRLITAGGTGGVVAIDQRGNIATPFNTEGMYRATRKNNEPAQVMIWSDL
HHHHHHHHHEEEEECCCCCEEEEECCCCCCCCCCCCCCEEHHCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]