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The map label for this gene is ycdT [H]

Identifier: 167623607

GI number: 167623607

Start: 2008400

End: 2010241

Strand: Direct

Name: ycdT [H]

Synonym: Shal_1676

Alternate gene names: 167623607

Gene position: 2008400-2010241 (Clockwise)

Preceding gene: 167623603

Following gene: 167623608

Centisome position: 38.42

GC content: 44.84

Gene sequence:

>1842_bases
TTGGCTCTGATAGAGGATAAAACCCTCGATGCTGAGTTCAAACGCCAACAATTAAACCGTCTTATCTCTAAAGATCAAGC
TGAGCAAGATGCCTATTATGCCTTTGCTTACTATCGGATATTACATGATATTGAGCTCTCTAGTGGTGAGCCTCAGCGTG
CAGAGCAAGCCCTAGAAAGTATGGAGGCCGTTGGAAAGTCAAAGCAACAAGGGTGGTTGATTGCTGAGTCTAAGATGTGG
CAGGCGACTTTTGCGGCGAAGCAGAGTCGCTTTGATGATGGTTTAGCACTGGTTGATGAGTCGATACGCTTGTCAACACT
GGCAAGTTTTCACCATCTCACGGGACGAGCCTATAACACTAAGGCCGCGCTCTACTACTTCCAAGATCAGTACCATGTCG
CACTTGAGTATTATTTAAAAGCCCTAGATATTTTTAGATTACAGCCAGCCGACCCTTATGTTAGCAAGGTGCTATCTAAT
GTAGCGATTATCTATGTCGATTTAGAACAGTGGGATAAAGCTTGGGAGTCAAATAACGAGGCTCTGGCACATGTTGACCA
ATATGGTGGTAGCTATGAGCAGTTCAGTGCCTTTAGTAACAATGCCGCATTTATATTAGAGCGATTAGGTAGGGTTAGAG
AGAGTGAGCCTTATTTAATTGCCGCACGAAAAAACGCTGAATTAAGTGGAAATTTGCGAGTGATAATGAACGCTAAAACT
TCTTGGGCTCATTTCTTTTTCTCTACAGAGCAATATCAGCAGGCCGTGGATGTCGGACGAGAGTGCATTGCAATTTCCTC
ATCTAATCAATACCCACTGTTTGAAAGTGACTGTAGTCGAATAGTGGCAAAGGCTTTAGTTAAGCTGGGTCGAGTCGATG
AGGCTTTGTTATCACTTGCATATAGCGAGGCCCTGTATAAGCAGATAGGAGTACGCTCCGGTTTAGCTGATACGTTCAAT
ACATACGCACTGGCTTATGAGGCAATCGGTGATTACAAAGCGGCGCTGAACTATCAGCGCAAAGCGAGTGAAGAAGATAA
AGCCTTACTGTTTGATAGGCGGGCAAAGTTAAGCCTTAATCTTAGTCAAAGTTATCAACAGAAGTATCGGCAGCAAGAAC
TCGCAGTGTTAACGGCTGAAAATGATGCCCACGAAGCCCGTCTTGCGGAGCAGCATCTTCGTGAAAAGTTTCTGTTCTTT
GTTATTCTCGTCGCCATCATCTCCATTTCCCTGTTGATTAAAAAACGCTATGGATTGGAAAACGATAATAAGAATTTACA
AAGCTCTAATCGTGAGCTTTATAAACAGTCCAACCTCGACAGCTTAACGGGCCTATATAACCGCCGTTATTTCTTAGACT
ATTTAGCACAGCAAGGACAACTGCCAACACTCCAAAATATGAGAATGTGTTTAGCGATTATCGATATTGATCATTTTAAG
AGGGTCAATGACACCTATGGCCATGACGTGGGTGATGAGGTATTGGTTAGAGTCGGGCAACTGATCCAGAGCAACATTCG
TGAAGATGACCTACTTATTCGCTGGGGTGGCGAAGAGTTTATCTTGTTGCTTAGCTGGCCTGACGTTTCCTGTCCTCAAT
CTGTGCGAGCACTATCGAACCATTTTGAACGTATTCGTCAGGCTGTAGCTGATGCCATCATTGAGGTGCAAGAGCAGCAA
CTGCACTTGACGGTTTCGGTTGGGGTGGGGCAGCCATTAGATGCGCGAACCTTAGTCGAGTCTTGGCAGTTTGTGCTTGA
TTGTGCCGATAAGGCGCTCTATCTCGCTAAGAGGCAAGGACGCAATTGTGTGGTACTCGCTAAGGAAGCATCGTTCACAT
AG

Upstream 100 bases:

>100_bases
TTATTGTTTTTATTGGAATGTCATTCTATTCATGCTCGGCTAATCAGCTGAGTTTTCTCCTTTCATAAAAATTCAGAAAT
TTAGTCAGGGACAAGCAATC

Downstream 100 bases:

>100_bases
GGAAGTGATTGAAGATCAATAATAAAGTAATAGTGCTTTAGCTAGCTTCGGGACAAAGTTTGTATTGACCAGCTTTTATT
CAGCTTTTAGTCTGTAAGCT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MALIEDKTLDAEFKRQQLNRLISKDQAEQDAYYAFAYYRILHDIELSSGEPQRAEQALESMEAVGKSKQQGWLIAESKMW
QATFAAKQSRFDDGLALVDESIRLSTLASFHHLTGRAYNTKAALYYFQDQYHVALEYYLKALDIFRLQPADPYVSKVLSN
VAIIYVDLEQWDKAWESNNEALAHVDQYGGSYEQFSAFSNNAAFILERLGRVRESEPYLIAARKNAELSGNLRVIMNAKT
SWAHFFFSTEQYQQAVDVGRECIAISSSNQYPLFESDCSRIVAKALVKLGRVDEALLSLAYSEALYKQIGVRSGLADTFN
TYALAYEAIGDYKAALNYQRKASEEDKALLFDRRAKLSLNLSQSYQQKYRQQELAVLTAENDAHEARLAEQHLREKFLFF
VILVAIISISLLIKKRYGLENDNKNLQSSNRELYKQSNLDSLTGLYNRRYFLDYLAQQGQLPTLQNMRMCLAIIDIDHFK
RVNDTYGHDVGDEVLVRVGQLIQSNIREDDLLIRWGGEEFILLLSWPDVSCPQSVRALSNHFERIRQAVADAIIEVQEQQ
LHLTVSVGVGQPLDARTLVESWQFVLDCADKALYLAKRQGRNCVVLAKEASFT

Sequences:

>Translated_613_residues
MALIEDKTLDAEFKRQQLNRLISKDQAEQDAYYAFAYYRILHDIELSSGEPQRAEQALESMEAVGKSKQQGWLIAESKMW
QATFAAKQSRFDDGLALVDESIRLSTLASFHHLTGRAYNTKAALYYFQDQYHVALEYYLKALDIFRLQPADPYVSKVLSN
VAIIYVDLEQWDKAWESNNEALAHVDQYGGSYEQFSAFSNNAAFILERLGRVRESEPYLIAARKNAELSGNLRVIMNAKT
SWAHFFFSTEQYQQAVDVGRECIAISSSNQYPLFESDCSRIVAKALVKLGRVDEALLSLAYSEALYKQIGVRSGLADTFN
TYALAYEAIGDYKAALNYQRKASEEDKALLFDRRAKLSLNLSQSYQQKYRQQELAVLTAENDAHEARLAEQHLREKFLFF
VILVAIISISLLIKKRYGLENDNKNLQSSNRELYKQSNLDSLTGLYNRRYFLDYLAQQGQLPTLQNMRMCLAIIDIDHFK
RVNDTYGHDVGDEVLVRVGQLIQSNIREDDLLIRWGGEEFILLLSWPDVSCPQSVRALSNHFERIRQAVADAIIEVQEQQ
LHLTVSVGVGQPLDARTLVESWQFVLDCADKALYLAKRQGRNCVVLAKEASFT
>Mature_612_residues
ALIEDKTLDAEFKRQQLNRLISKDQAEQDAYYAFAYYRILHDIELSSGEPQRAEQALESMEAVGKSKQQGWLIAESKMWQ
ATFAAKQSRFDDGLALVDESIRLSTLASFHHLTGRAYNTKAALYYFQDQYHVALEYYLKALDIFRLQPADPYVSKVLSNV
AIIYVDLEQWDKAWESNNEALAHVDQYGGSYEQFSAFSNNAAFILERLGRVRESEPYLIAARKNAELSGNLRVIMNAKTS
WAHFFFSTEQYQQAVDVGRECIAISSSNQYPLFESDCSRIVAKALVKLGRVDEALLSLAYSEALYKQIGVRSGLADTFNT
YALAYEAIGDYKAALNYQRKASEEDKALLFDRRAKLSLNLSQSYQQKYRQQELAVLTAENDAHEARLAEQHLREKFLFFV
ILVAIISISLLIKKRYGLENDNKNLQSSNRELYKQSNLDSLTGLYNRRYFLDYLAQQGQLPTLQNMRMCLAIIDIDHFKR
VNDTYGHDVGDEVLVRVGQLIQSNIREDDLLIRWGGEEFILLLSWPDVSCPQSVRALSNHFERIRQAVADAIIEVQEQQL
HLTVSVGVGQPLDARTLVESWQFVLDCADKALYLAKRQGRNCVVLAKEASFT

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=39.3063583815029, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI145693134, Length=209, Percent_Identity=32.0574162679426, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI87081881, Length=169, Percent_Identity=39.0532544378698, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=35.5932203389831, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=36.4161849710983, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787816, Length=179, Percent_Identity=34.0782122905028, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1787802, Length=181, Percent_Identity=33.1491712707182, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI87082007, Length=214, Percent_Identity=30.3738317757009, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87081977, Length=126, Percent_Identity=34.9206349206349, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=33.5294117647059, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=31.0975609756098, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=34.8066298342541, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 70033; Mature: 69902

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50293 TPR_REGION ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIEDKTLDAEFKRQQLNRLISKDQAEQDAYYAFAYYRILHDIELSSGEPQRAEQALES
CCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MEAVGKSKQQGWLIAESKMWQATFAAKQSRFDDGLALVDESIRLSTLASFHHLTGRAYNT
HHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCC
KAALYYFQDQYHVALEYYLKALDIFRLQPADPYVSKVLSNVAIIYVDLEQWDKAWESNNE
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEHHHHHHHHHCCCH
ALAHVDQYGGSYEQFSAFSNNAAFILERLGRVRESEPYLIAARKNAELSGNLRVIMNAKT
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCC
SWAHFFFSTEQYQQAVDVGRECIAISSSNQYPLFESDCSRIVAKALVKLGRVDEALLSLA
CHHHEEECHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YSEALYKQIGVRSGLADTFNTYALAYEAIGDYKAALNYQRKASEEDKALLFDRRAKLSLN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHCCCCCEEEC
LSQSYQQKYRQQELAVLTAENDAHEARLAEQHLREKFLFFVILVAIISISLLIKKRYGLE
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDNKNLQSSNRELYKQSNLDSLTGLYNRRYFLDYLAQQGQLPTLQNMRMCLAIIDIDHFK
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RVNDTYGHDVGDEVLVRVGQLIQSNIREDDLLIRWGGEEFILLLSWPDVSCPQSVRALSN
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHH
HFERIRQAVADAIIEVQEQQLHLTVSVGVGQPLDARTLVESWQFVLDCADKALYLAKRQG
HHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RNCVVLAKEASFT
CCEEEEECCCCCC
>Mature Secondary Structure 
ALIEDKTLDAEFKRQQLNRLISKDQAEQDAYYAFAYYRILHDIELSSGEPQRAEQALES
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MEAVGKSKQQGWLIAESKMWQATFAAKQSRFDDGLALVDESIRLSTLASFHHLTGRAYNT
HHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCC
KAALYYFQDQYHVALEYYLKALDIFRLQPADPYVSKVLSNVAIIYVDLEQWDKAWESNNE
CEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEHHHHHHHHHCCCH
ALAHVDQYGGSYEQFSAFSNNAAFILERLGRVRESEPYLIAARKNAELSGNLRVIMNAKT
HHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEEECCC
SWAHFFFSTEQYQQAVDVGRECIAISSSNQYPLFESDCSRIVAKALVKLGRVDEALLSLA
CHHHEEECHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHH
YSEALYKQIGVRSGLADTFNTYALAYEAIGDYKAALNYQRKASEEDKALLFDRRAKLSLN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCHHHHHCCCCCEEEC
LSQSYQQKYRQQELAVLTAENDAHEARLAEQHLREKFLFFVILVAIISISLLIKKRYGLE
HHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NDNKNLQSSNRELYKQSNLDSLTGLYNRRYFLDYLAQQGQLPTLQNMRMCLAIIDIDHFK
CCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RVNDTYGHDVGDEVLVRVGQLIQSNIREDDLLIRWGGEEFILLLSWPDVSCPQSVRALSN
HCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCCCCCHHHHHHHH
HFERIRQAVADAIIEVQEQQLHLTVSVGVGQPLDARTLVESWQFVLDCADKALYLAKRQG
HHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RNCVVLAKEASFT
CCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]