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The map label for this gene is fdnG [H]

Identifier: 167622432

GI number: 167622432

Start: 592862

End: 593443

Strand: Direct

Name: fdnG [H]

Synonym: Shal_0492

Alternate gene names: 167622432

Gene position: 592862-593443 (Clockwise)

Preceding gene: 167622431

Following gene: 167622433

Centisome position: 11.34

GC content: 49.48

Gene sequence:

>582_bases
ATGGACAGACGACAGTTTTTTAAACTGTGTGCCTGCGGAGCGACCACATCGGCAATATCAGCGCTGGGACTAATGCCCGG
CACTGCGATGGCGGCGCCTCGTGAGTACAAGTTGTTGAGAGCAAAAGAGACCCGCAATAACTGCTGTTATTGTTCAGTGG
GTTGTGGCTTGCTGATGTACAGCAATGGCAGTAACGGCAAAAATGCCGAACAAGCAATTTTCCACATTGAAGGCGACGCT
GACCATCCGGTTAACCGCGGCGCATTATGCCCTAAAGGTGCAGGATTGGTGGACTATGTAAATAGCCCTAATCGTAACCA
TTTCCCTGAAGTGCGTGAGGCTGGTTCTAACGAGTGGAAACGCATTAGCTGGAATGAAGCATTTGGTCGTATCGCGCGTT
TAATGAAAGATGACCGCGATGCAAACCTAATTGAAAAGAACAGTGACGGTACCACAGTAAACCGTTGGTTGAGCACAGGC
ATGATGACCTCGTCTGCACAGCCAAACGAGGGTGGTTTTATCACTCATAAGTTTGCTCGTTCTCTTGGCCTAGTGGCCAT
CGACACTATTGCGCGTAACTGA

Upstream 100 bases:

>100_bases
GCACAAGTTGGACTAAAAGTGGATTAACGGAAAAACCAAAACTACATGCCGAGATGAGTTAGTCGGTCAGAGTAGAAAAT
ACTAATTGTGTGAGGGTGAT

Downstream 100 bases:

>100_bases
CACTCCCCAACGGTAGCAAGTCTTGCTCCAACATTTGGGCGCGGTGCCATGACCAACCACTGGATCGATATTAAGAACGC
AAATGTCGTGGTGATCATGG

Product: molybdopterin oxidoreductase Fe4S4 region

Products: NA

Alternate protein names: Anaerobic formate dehydrogenase major subunit; Formate dehydrogenase-N subunit alpha; FDH-N subunit alpha [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MDRRQFFKLCACGATTSAISALGLMPGTAMAAPREYKLLRAKETRNNCCYCSVGCGLLMYSNGSNGKNAEQAIFHIEGDA
DHPVNRGALCPKGAGLVDYVNSPNRNHFPEVREAGSNEWKRISWNEAFGRIARLMKDDRDANLIEKNSDGTTVNRWLSTG
MMTSSAQPNEGGFITHKFARSLGLVAIDTIARN

Sequences:

>Translated_193_residues
MDRRQFFKLCACGATTSAISALGLMPGTAMAAPREYKLLRAKETRNNCCYCSVGCGLLMYSNGSNGKNAEQAIFHIEGDA
DHPVNRGALCPKGAGLVDYVNSPNRNHFPEVREAGSNEWKRISWNEAFGRIARLMKDDRDANLIEKNSDGTTVNRWLSTG
MMTSSAQPNEGGFITHKFARSLGLVAIDTIARN
>Mature_193_residues
MDRRQFFKLCACGATTSAISALGLMPGTAMAAPREYKLLRAKETRNNCCYCSVGCGLLMYSNGSNGKNAEQAIFHIEGDA
DHPVNRGALCPKGAGLVDYVNSPNRNHFPEVREAGSNEWKRISWNEAFGRIARLMKDDRDANLIEKNSDGTTVNRWLSTG
MMTSSAQPNEGGFITHKFARSLGLVAIDTIARN

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The alpha subunit forms the active site [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI3868719, Length=192, Percent_Identity=66.1458333333333, Blast_Score=274, Evalue=2e-75,
Organism=Escherichia coli, GI3868720, Length=192, Percent_Identity=63.0208333333333, Blast_Score=268, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006443
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 21121; Mature: 21121

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRQFFKLCACGATTSAISALGLMPGTAMAAPREYKLLRAKETRNNCCYCSVGCGLLMY
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCHHHHEEHHCCCCCCEEEECCCCEEEE
SNGSNGKNAEQAIFHIEGDADHPVNRGALCPKGAGLVDYVNSPNRNHFPEVREAGSNEWK
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCE
RISWNEAFGRIARLMKDDRDANLIEKNSDGTTVNRWLSTGMMTSSAQPNEGGFITHKFAR
EECHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHH
SLGLVAIDTIARN
HCCCEEEHHHHCC
>Mature Secondary Structure
MDRRQFFKLCACGATTSAISALGLMPGTAMAAPREYKLLRAKETRNNCCYCSVGCGLLMY
CCHHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCHHHHEEHHCCCCCCEEEECCCCEEEE
SNGSNGKNAEQAIFHIEGDADHPVNRGALCPKGAGLVDYVNSPNRNHFPEVREAGSNEWK
ECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCCCCCE
RISWNEAFGRIARLMKDDRDANLIEKNSDGTTVNRWLSTGMMTSSAQPNEGGFITHKFAR
EECHHHHHHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCCCCCCEEHHHHHH
SLGLVAIDTIARN
HCCCEEEHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]