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The map label for this gene is acnB [H]

Identifier: 167622422

GI number: 167622422

Start: 577723

End: 580320

Strand: Direct

Name: acnB [H]

Synonym: Shal_0482

Alternate gene names: 167622422

Gene position: 577723-580320 (Clockwise)

Preceding gene: 167622420

Following gene: 167622424

Centisome position: 11.05

GC content: 48.69

Gene sequence:

>2598_bases
GTGCTAGAAGCATATCGTAAACACGTCGCAGAACGTGCTTCAGAGGGCGTAGTCCCTAAGCCATTAGATGCCCAACAAGT
GGCACAGCTAGTCGAATTGGTAAAGAATCCACCAGCAGGTGAAGAAGCATTTATTCTCGATCTACTTGAAAACCGTATCC
CACCGGGTGTAGATGAAGCCGCATACGTTAAAGCGGGTTTCTTAGATGCTGTCGCAAAATGCGAAATAGCATCTCCTATC
TTATCGTCTCAGCGCGCAGTTGAGCTGCTTGGCACGATGCAAGGTGGTTATAACATCGAGCCGCTAATTGCTCAGTTAGA
TAATGATGCGCAAGCCCCACTTGCCGTTAAAGCATTGTCACATACACTTTTGATGTTTGACTCTTTCCACGATGTAGTGG
ACAAAATGCAAGCGGGTAACGACTACGCTAAGCAAGTCGTCCATGCTTGGGCTGATGCTAAATGGTTCCTATCTCGCCCC
AAGCTTGCCGAGAAAATCTCTCTGACCGTATTTAAAGTATCGGGTGAAACCAACACCGATGACTTGTCTCCAGCACCAGA
TGCATGGTCACGCCCTGATATTCCATTGCATGCACTCGCGATGCTTAAGAATGCTCGGGACGGCATTGTGCCTGATGAAG
CTGGCGTAGTGGGCCCAATCAAAGAGATTGAATCACTAAAGACTAAAGGCTTCCCACTGGTTTATGTCGGTGATGTTGTG
GGTACCGGTTCTTCACGTAAATCTGCAACCAACTCCGTGCTGTGGTTCATGGGAGATGATATCCCGAATGTACCTAATAA
GCGCGCTGGTGGTTTCTGTTTAGGTGGCAAGATTGCCCCAATCTTCTTCAACACCATGGAAGATGCAGGTGCACTACCGA
TTGAGCTAGATGTTAGCAAGATGGAGATGGGCGATGTTATCGACATCTATCCATATGAAGGCATCGTTAAGCGTCATGGC
ACTGATGAAGTGATTTCACAGTTCTCTCTAAAGACAGATGTGTTGATGGATGAAGTGCGTGCGGGTGGCCGTATTCCATT
GATTATCGGTCGTGGCCTAACCGATCGTGCTCGTGAAACGCTAAACCTTGAAGCATCAACTGAGTTTGTTCGTCCAAAGG
ATGTGGCAGACACGGGTAAGGGCTTCACCCTTGCGCAGAAGATGGTGGGTAAGGCATGTGGCGTTACCGGTATTCGCCCT
GGCCAATATTGTGAACCTAAGATGACCTCTGTTGGCTCTCAAGATACTACCGGTCCTATGACGCGTGATGAGCTTAAAGA
TTTAGCTTGTCTTGGTTTTAGTGCCGATTTGACCATGCAGTCTTTCTGTCACACAGCGGCTTATCCAAAGCCAGTTGACG
TGAACACGCACCACACACTGCCAGATTTCATCATGAATCGCGGTGGTGTTTCACTACGTCCTGGTGACGGTGTTATTCAC
TCTTGGTTAAACCGCATGCTACTGCCTGATACCGTGGGTACGGGTGGTGACTCTCATACGCGCTTCCCAATCGGTATCTC
GTTCCCTGCAGGTTCTGGCCTTGTGGCGTTTGCTGCTGCAACAGGTGTCATGCCTCTTGATATGCCTGAATCAATCTTGG
TTCGCTTTAAGGGTGAGATGCAGCCTGGTATTACATTACGTGACTTGGTACATGCGATTCCACATAAAGCCATTGAGATG
GGACTGCTAACCGTTGAGAAGAAGGGTAAGATCAATGCCTTCTCGGGTCGCGTTCTAGAGATCGAAGGTCTTGAGCAGCT
TAAAGTTGAGCAGGCATTTGAACTTTCAGATGCATCGGCTGAGCGTAGTGCGGCGGGCTGTACTATTAAGCTAGATAAAG
AACCGATTATTGAATACCTAAACTCAAACATCGTCATGCTTAAGTGGATGATTAGCGAGGGGTATGGCGATCGTCGCACC
ATCGAACGTCGTATTACAGCGATGCAAGAGTGGTTAGCAACCCCAGAGCTAATGGAGGCGGACAGCGATGCTGAGTATGC
TGAAGTTATCGAAATCGATTTGAACGAAATCAAAGAGCCTATTCTTTGTGCGCCAAATGATCCAGATGATGCCATCTTAC
TGTCTTCAGTGGTTAATACTAAGATCGACGAAGTATTCGTAGGTTCTTGTATGACCAACATCGGTCACTTCCGCGCGACG
GGTAAGATGCTAGATAAGTTTGCATCGACGCTGCCAACACGTCTGTGGATCGCTCCACCGACCAAGATGGATCGCGATCA
GCTTACTGAGGAAGGCTACTACGCCATCTTCGGACGCGTGGGCGCTCGAATTGAGATCCCGGGTTGTTCATTGTGTATGG
GTAACCAAGCACGTGTTGCCGAAGGTGCGACTGTAGTTTCAACATCTACACGTAACTTCCCGAACCGTCTAGGTACAGGT
GCTAACGTATACTTAGCCTCGGCAGAGCTCGCGTCCGTCGCTGCGCTACTGGGTCGTCTACCATCACCTGATGAATATCA
GACATACGCGAAAGAGTTAGATGCGACTGCAGCCGACACTTACCGTTACCTGAGCTTCGATAAGATAGCATCGTACACGG
CAAAAGCTGACGAAGTGATTTTCCAAACGGCGGTTTAA

Upstream 100 bases:

>100_bases
AATGGCGCTCGAAATAAGGGGCGAGCCTGCCGAGCTGTCAGTTTCAGTTTGAAGACGAGTAGTTTCGCTGTTAGAACGCC
AAACAAAGAGGAAGGTTGCC

Downstream 100 bases:

>100_bases
GCCTGATTGATACACTGAAGTTGATGCTCTGAAATAGAAAAACTAAAAAGGCCTGCTAATTAGCAGGCCTTTTTGTTAAT
TCATTTTAAATTTTTGCGAG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKCEIASPI
LSSQRAVELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDSFHDVVDKMQAGNDYAKQVVHAWADAKWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEAGVVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGIVKRHG
TDEVISQFSLKTDVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPKDVADTGKGFTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLATPELMEADSDAEYAEVIEIDLNEIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELASVAALLGRLPSPDEYQTYAKELDATAADTYRYLSFDKIASYTAKADEVIFQTAV

Sequences:

>Translated_865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKCEIASPI
LSSQRAVELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDSFHDVVDKMQAGNDYAKQVVHAWADAKWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEAGVVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGIVKRHG
TDEVISQFSLKTDVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPKDVADTGKGFTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLATPELMEADSDAEYAEVIEIDLNEIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELASVAALLGRLPSPDEYQTYAKELDATAADTYRYLSFDKIASYTAKADEVIFQTAV
>Mature_865_residues
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEAAYVKAGFLDAVAKCEIASPI
LSSQRAVELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALSHTLLMFDSFHDVVDKMQAGNDYAKQVVHAWADAKWFLSRP
KLAEKISLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEAGVVGPIKEIESLKTKGFPLVYVGDVV
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYEGIVKRHG
TDEVISQFSLKTDVLMDEVRAGGRIPLIIGRGLTDRARETLNLEASTEFVRPKDVADTGKGFTLAQKMVGKACGVTGIRP
GQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEMQPGITLRDLVHAIPHKAIEM
GLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRT
IERRITAMQEWLATPELMEADSDAEYAEVIEIDLNEIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTG
ANVYLASAELASVAALLGRLPSPDEYQTYAKELDATAADTYRYLSFDKIASYTAKADEVIFQTAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=80.5780346820809, Blast_Score=1434, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=385, Percent_Identity=24.4155844155844, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=25.9842519685039, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321429, Length=359, Percent_Identity=23.6768802228412, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93833; Mature: 93833

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAVAKCEIASPILSSQRAVELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHH
HTLLMFDSFHDVVDKMQAGNDYAKQVVHAWADAKWFLSRPKLAEKISLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCHHHEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEAGVVGPIKEIESLKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYEGIVKRHGTDEVISQFSLKTDVLMDEVRAGGRIPLIIGRGLTDRARET
HCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LNLEASTEFVRPKDVADTGKGFTLAQKMVGKACGVTGIRPGQYCEPKMTSVGSQDTTGPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVHAIPHKAIEMGLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASA
CCCCCHHHHHHHCCHHHHHHCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRTIERRITAMQEWLATPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCC
DSDAEYAEVIEIDLNEIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
CCCCCCHHHEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELASVAALLGRLPSPDEYQTYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
YRYLSFDKIASYTAKADEVIFQTAV
HHHHHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MLEAYRKHVAERASEGVVPKPLDAQQVAQLVELVKNPPAGEEAFILDLLENRIPPGVDEA
CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCH
AYVKAGFLDAVAKCEIASPILSSQRAVELLGTMQGGYNIEPLIAQLDNDAQAPLAVKALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCHHHHHHH
HTLLMFDSFHDVVDKMQAGNDYAKQVVHAWADAKWFLSRPKLAEKISLTVFKVSGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHCCCCHHHEEEEEEEEECCCCCCC
DLSPAPDAWSRPDIPLHALAMLKNARDGIVPDEAGVVGPIKEIESLKTKGFPLVYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEE
GTGSSRKSATNSVLWFMGDDIPNVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEEEEECHHCCCCCEEEEEHHH
MEMGDVIDIYPYEGIVKRHGTDEVISQFSLKTDVLMDEVRAGGRIPLIIGRGLTDRARET
HCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
LNLEASTEFVRPKDVADTGKGFTLAQKMVGKACGVTGIRPGQYCEPKMTSVGSQDTTGPM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCCCC
TRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNTHHTLPDFIMNRGGVSLRPGDGVIH
CHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCEECCCCCHHH
SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGEM
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEHHHCCCCCCCCCHHHHHEECCCC
QPGITLRDLVHAIPHKAIEMGLLTVEKKGKINAFSGRVLEIEGLEQLKVEQAFELSDASA
CCCCCHHHHHHHCCHHHHHHCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHCCCCCC
ERSAAGCTIKLDKEPIIEYLNSNIVMLKWMISEGYGDRRTIERRITAMQEWLATPELMEA
HHCCCCCEEEECHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHCC
DSDAEYAEVIEIDLNEIKEPILCAPNDPDDAILLSSVVNTKIDEVFVGSCMTNIGHFRAT
CCCCCCHHHEEEEHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GKMLDKFASTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQARVA
HHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEEEEECCCCEEECCCCCEECCCCCCCC
EGATVVSTSTRNFPNRLGTGANVYLASAELASVAALLGRLPSPDEYQTYAKELDATAADT
CCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHH
YRYLSFDKIASYTAKADEVIFQTAV
HHHHHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]