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The map label for this gene is murD [H]

Identifier: 167622393

GI number: 167622393

Start: 531711

End: 533030

Strand: Direct

Name: murD [H]

Synonym: Shal_0453

Alternate gene names: 167622393

Gene position: 531711-533030 (Clockwise)

Preceding gene: 167622392

Following gene: 167622394

Centisome position: 10.17

GC content: 47.73

Gene sequence:

>1320_bases
ATGGCTCAGCAATATTCACACATAGTTTTAGGTTTAGGCGCAACAGGTTTATCTGTAGTGCGTTACCTGTGTGGGCAAGG
CATCGTTCCTTTAGTGATGGATAGCCGAAACCAGCCTCCAGGGGCTGACACATTAGCAGCAGAGTTTCCTCAAGTTGAGT
TGGTAACTGGCGGTTTTGACTGTCGTTACTTAGTACAGGCACAGCAGATCATTATTAGTCCTGGTATTGCGTGTGAAACA
CCTGAAGTCAGGGTTGCATTCGATATGGGGATCGAGGTGATCGGTGATGTTGAACTTTTTGCTCGAGAGGTGAGCCAAGG
TTCTGCCAAGGTTATCGGGATCACAGGCTCTAATGGCAAGACCACTGTCACTACCTTAGTGGGTGAGATGGCTAAAGCCT
GTGGCTGCTCTTATGCCGTCGGTGGCAATATTGGTGTGCCTGCACTGGATCTACTGCTCGAGCCTAAAGAGTTATATATC
TTGGAGCTATCGAGTTTTCAGCTAGAGACAACCCATAGCTTGCAGTGTACTGCAGCAACGTGTCTCAACATTAGCGAAGA
TCATATGGATCGTTACAGCGATCTAGAGTCATATCGTCAGGCCAAGCTTAAGCTGTATAAGCAGAGCAAAATGGTGCTGT
TTAACCGTGAAGATGCGTTAACAGTACCGAATAACCCAACCAACCAAGAAAGTTTTGGTTTAGATGCGCCAGAAATGGAT
GCATGGGGAATTTCTGGCGGAATGATAGTACATGGTAGTAGCCAGATCATGGCCGTTAGCGATGTGGCGCTAGTGGGTAG
TCATAACCACGCCAATATCATTGCAGCGATGGCGTTAGCGACTGCCGCAGGTATTGAACGAGACTTTCAAATTGAGGTGG
CTAAGACGTTTTCAGGTCTTGCCCATCGCTGCGAAGTGGTTGCTGAAATTGCCGGTGTCACGTATGTCAACGACTCTAAG
GCAACCAATATTGGTGCTACGGTCGCAGCCATTGACGGCTTAAGCGAGCACTTAGGTGAACTGATTTTAATCGCCGGCGG
AGACGGTAAAGGCGCAGACTTTAAACCGTTAGAGCCTGTGCTTGAGAAGGTGAGTCAGCTAATCGTTTTAGGCAAAGACG
CTGACAAGATCGCTGCATTAAAACCTGAGGCGATAAAAGTGACCACGATGGCAGAAGCTGTGCTGAAAGCCAATGAACTT
GCCACTGCAGGTGATATTGTTATTTTATCACCCGCTTGCTCTAGCTTAGATATGTATCCTAATTTTATGGCGCGTGGCGA
TGAATTTCGCAGCTTGGTAGAAGCGCTTCATGGCGAGTAG

Upstream 100 bases:

>100_bases
TCATCGTTCGTTTTTGGATTATCTCCTTGTTCCTAGTCCTACTTGGACTGGCAACCTTGAAACTTAGATAACGGATTAAA
CTGGATTAGATAGGTCTGAT

Downstream 100 bases:

>100_bases
TGAGAAGCAGTTAAGCCTATTTAGCAACCGCTTTAAGTGGTCGTTGCCGAATTTGTTTAATGATAACTCTGCTCCAGGGA
TGCAGCTGTACGATAGAGCA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAQQYSHIVLGLGATGLSVVRYLCGQGIVPLVMDSRNQPPGADTLAAEFPQVELVTGGFDCRYLVQAQQIIISPGIACET
PEVRVAFDMGIEVIGDVELFAREVSQGSAKVIGITGSNGKTTVTTLVGEMAKACGCSYAVGGNIGVPALDLLLEPKELYI
LELSSFQLETTHSLQCTAATCLNISEDHMDRYSDLESYRQAKLKLYKQSKMVLFNREDALTVPNNPTNQESFGLDAPEMD
AWGISGGMIVHGSSQIMAVSDVALVGSHNHANIIAAMALATAAGIERDFQIEVAKTFSGLAHRCEVVAEIAGVTYVNDSK
ATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDADKIAALKPEAIKVTTMAEAVLKANEL
ATAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE

Sequences:

>Translated_439_residues
MAQQYSHIVLGLGATGLSVVRYLCGQGIVPLVMDSRNQPPGADTLAAEFPQVELVTGGFDCRYLVQAQQIIISPGIACET
PEVRVAFDMGIEVIGDVELFAREVSQGSAKVIGITGSNGKTTVTTLVGEMAKACGCSYAVGGNIGVPALDLLLEPKELYI
LELSSFQLETTHSLQCTAATCLNISEDHMDRYSDLESYRQAKLKLYKQSKMVLFNREDALTVPNNPTNQESFGLDAPEMD
AWGISGGMIVHGSSQIMAVSDVALVGSHNHANIIAAMALATAAGIERDFQIEVAKTFSGLAHRCEVVAEIAGVTYVNDSK
ATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDADKIAALKPEAIKVTTMAEAVLKANEL
ATAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE
>Mature_438_residues
AQQYSHIVLGLGATGLSVVRYLCGQGIVPLVMDSRNQPPGADTLAAEFPQVELVTGGFDCRYLVQAQQIIISPGIACETP
EVRVAFDMGIEVIGDVELFAREVSQGSAKVIGITGSNGKTTVTTLVGEMAKACGCSYAVGGNIGVPALDLLLEPKELYIL
ELSSFQLETTHSLQCTAATCLNISEDHMDRYSDLESYRQAKLKLYKQSKMVLFNREDALTVPNNPTNQESFGLDAPEMDA
WGISGGMIVHGSSQIMAVSDVALVGSHNHANIIAAMALATAAGIERDFQIEVAKTFSGLAHRCEVVAEIAGVTYVNDSKA
TNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPVLEKVSQLIVLGKDADKIAALKPEAIKVTTMAEAVLKANELA
TAGDIVILSPACSSLDMYPNFMARGDEFRSLVEALHGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=443, Percent_Identity=48.0812641083521, Blast_Score=353, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 46431; Mature: 46299

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQQYSHIVLGLGATGLSVVRYLCGQGIVPLVMDSRNQPPGADTLAAEFPQVELVTGGFD
CCCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCEEEEEECCCC
CRYLVQAQQIIISPGIACETPEVRVAFDMGIEVIGDVELFAREVSQGSAKVIGITGSNGK
EEEHEEEHHHEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEEEEECCCCC
TTVTTLVGEMAKACGCSYAVGGNIGVPALDLLLEPKELYILELSSFQLETTHSLQCTAAT
CHHHHHHHHHHHHHCCCEECCCCCCCCHHHHEECCCCEEEEEECCEEEECCCCEEEEEHH
CLNISEDHMDRYSDLESYRQAKLKLYKQSKMVLFNREDALTVPNNPTNQESFGLDAPEMD
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCC
AWGISGGMIVHGSSQIMAVSDVALVGSHNHANIIAAMALATAAGIERDFQIEVAKTFSGL
CCCCCCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
AHRCEVVAEIAGVTYVNDSKATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPV
HHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
LEKVSQLIVLGKDADKIAALKPEAIKVTTMAEAVLKANELATAGDIVILSPACSSLDMYP
HHHHHHEEEECCCCCHHEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
NFMARGDEFRSLVEALHGE
HHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AQQYSHIVLGLGATGLSVVRYLCGQGIVPLVMDSRNQPPGADTLAAEFPQVELVTGGFD
CCCCCEEEEEECCHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHCCCEEEEEECCCC
CRYLVQAQQIIISPGIACETPEVRVAFDMGIEVIGDVELFAREVSQGSAKVIGITGSNGK
EEEHEEEHHHEECCCCCCCCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEEEEECCCCC
TTVTTLVGEMAKACGCSYAVGGNIGVPALDLLLEPKELYILELSSFQLETTHSLQCTAAT
CHHHHHHHHHHHHHCCCEECCCCCCCCHHHHEECCCCEEEEEECCEEEECCCCEEEEEHH
CLNISEDHMDRYSDLESYRQAKLKLYKQSKMVLFNREDALTVPNNPTNQESFGLDAPEMD
HCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCC
AWGISGGMIVHGSSQIMAVSDVALVGSHNHANIIAAMALATAAGIERDFQIEVAKTFSGL
CCCCCCCEEEECCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEHHHHHHHH
AHRCEVVAEIAGVTYVNDSKATNIGATVAAIDGLSEHLGELILIAGGDGKGADFKPLEPV
HHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
LEKVSQLIVLGKDADKIAALKPEAIKVTTMAEAVLKANELATAGDIVILSPACSSLDMYP
HHHHHHEEEECCCCCHHEECCCCCEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
NFMARGDEFRSLVEALHGE
HHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA