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The map label for this gene is leuC

Identifier: 167622380

GI number: 167622380

Start: 517130

End: 518530

Strand: Direct

Name: leuC

Synonym: Shal_0440

Alternate gene names: 167622380

Gene position: 517130-518530 (Clockwise)

Preceding gene: 167622379

Following gene: 167622381

Centisome position: 9.89

GC content: 49.82

Gene sequence:

>1401_bases
ATGGCGAAGACATTGTATGAAAAGGTATGGGATAACCATATTGTGTTTGCTGGCGAAGGTGAGGCGCCAATCATCTATGT
AGATAGGCATTTAGTGCATGAGGTGACGTCTCCACAAGCCTTTAGTGGCTTAAAAGTGGCGGGCCGCAAGTTACGTGCGC
CAGAAAAAACCTTCGCCACCATGGATCACAATACTTCAACTACAAGTGCAAGTTTAGATGCGTTAAGCCCAATGGCGCGA
ACGCAAGTTGAAACGCTTGAGCGAAACTGTAAAGAGTTTGGCGTGCGTCTTTATGATATCCACCATAAAAACCAGGGTAT
TGTGCATGTGATGGGGCCGGAGTTAGGTATTACTCTACCGGGCACTGTGATTGTCTGTGGTGACTCCCACACCGCAACCC
ATGGCGCTTTTGGTGCGCTTGCCTTTGGTATTGGCACCTCGGAAGTTGAGCATGTGATGGCAACGCAGACTTTGCGTCAG
CTTAAAGCTAAGACGATGAAAATTGAGGTGCGTGGCAAAGTGGCTGATGGTATTACGGCTAAAGATATCGTGCTGGCGAT
TATTGGTAAAATTGGTATGGACGGCGGTACAGGCTACGTGGTCGAGTTCTGTGGTGAGGCGATTGAAGCCTTGTCGATGG
AGGGGCGGATGACGGTTTGTAATATGGCCATCGAAATGGGCGCTAAAGCGGGCATGATCGCACCCGATACCACTACTGCT
GAGTACTTAGAGGGTCGCGAGTTTGCACCTAAAGGAGCAAGTTGGGAGCAGGCGGTTGAAGCATGGAGTCAGTTAAAGAC
TGATGCCGATGCAGTATTTGATGCCACTGTTGTGATTGAAGCTAAAGATATTGCCCCGCAATTAACCTGGGGGACTAACC
CTGGCCAAGTGGTTGCGATTGATCAGCTTGTGCCTAACCCGTTAGATGCCACCAATCCTACCGTGCGTACCAGTATTGAA
AATGCCTTACAGTACGTTGATTTAACCGCAGGCACCTTGATGACAGATGTCAGTATTAACAAGGTGTTTATCGGTTCATG
TACTAATTCTCGCATTGAAGATCTGCGCGCTGCAGCGGTGCATGCCAAAGGCCGCAAGGTTGCTGACGGGGTTAAAGCTA
TCGTGGTACCCGGGTCGGGCCTAGTGAAAGAGCAAGCAGAAGCAGAGGGTTTAGATAAGATCTTTTTGGAAGCGGGCTTT
GAGTGGCGCTTACCGGGATGCTCTATGTGTTTGGCAATGAACGATGACAAGTTAGAGGTGGGCGATCGCTGCGCGTCGAC
CAGTAATCGTAATTTTGAAGGTCGACAAGGGCGTGGTAGCCGTACTCACTTAGTGAGCCCTGCCATGGCGGCAGCTGCAG
CCGTTGCAGGCCATTTTGTTGATATTCGTAAACCATACTAG

Upstream 100 bases:

>100_bases
TAACGGGTGAGCTACTCGCAATTGAAGAGCGCCATAATGCAAAATCAACCAGTGAGATGGGTGACTATATTGCCAAGCTT
GTGCGAGAAGGAGTGTAACC

Downstream 100 bases:

>100_bases
GAGAGCAGCATGCAACCATTTATTTCACATACAGGGCTTGCGGTTATCTTAGATAGTGCAAACGTCGATACCGACCAGAT
TATCCCTAAACAATTTTTGT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKVWDNHIVFAGEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTTSASLDALSPMAR
TQVETLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQ
LKAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDTTTA
EYLEGREFAPKGASWEQAVEAWSQLKTDADAVFDATVVIEAKDIAPQLTWGTNPGQVVAIDQLVPNPLDATNPTVRTSIE
NALQYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNDDKLEVGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Sequences:

>Translated_466_residues
MAKTLYEKVWDNHIVFAGEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTTSASLDALSPMAR
TQVETLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQ
LKAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDTTTA
EYLEGREFAPKGASWEQAVEAWSQLKTDADAVFDATVVIEAKDIAPQLTWGTNPGQVVAIDQLVPNPLDATNPTVRTSIE
NALQYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGF
EWRLPGCSMCLAMNDDKLEVGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
>Mature_465_residues
AKTLYEKVWDNHIVFAGEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFATMDHNTSTTSASLDALSPMART
QVETLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQL
KAKTMKIEVRGKVADGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDTTTAE
YLEGREFAPKGASWEQAVEAWSQLKTDADAVFDATVVIEAKDIAPQLTWGTNPGQVVAIDQLVPNPLDATNPTVRTSIEN
ALQYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGFE
WRLPGCSMCLAMNDDKLEVGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=365, Percent_Identity=27.1232876712329, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=27.2727272727273, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI41352693, Length=364, Percent_Identity=27.1978021978022, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=70.6896551724138, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=368, Percent_Identity=30.1630434782609, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87081781, Length=342, Percent_Identity=24.8538011695906, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25149337, Length=366, Percent_Identity=29.2349726775956, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32564738, Length=386, Percent_Identity=28.7564766839378, Blast_Score=118, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=448, Percent_Identity=27.9017857142857, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=28.8961038961039, Blast_Score=109, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=61.1940298507463, Blast_Score=565, Evalue=1e-162,
Organism=Saccharomyces cerevisiae, GI6320440, Length=426, Percent_Identity=27.2300469483568, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=398, Percent_Identity=28.1407035175879, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=30.3523035230352, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI281365315, Length=383, Percent_Identity=26.3707571801567, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17864292, Length=383, Percent_Identity=26.3707571801567, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI161076999, Length=385, Percent_Identity=26.7532467532467, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=27.5482093663912, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.5350877192982, Blast_Score=95, Evalue=8e-20,
Organism=Drosophila melanogaster, GI17137564, Length=441, Percent_Identity=26.3038548752834, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SHEHH (B0TQM2)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672674.1
- ProteinModelPortal:   B0TQM2
- SMR:   B0TQM2
- GeneID:   5905823
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0440
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   SHAL458817:SHAL_0440-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49868; Mature: 49736

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKVWDNHIVFAGEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCCCCCHHHEE
MDHNTSTTSASLDALSPMARTQVETLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP
ECCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGKVADGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDTTTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
EYLEGREFAPKGASWEQAVEAWSQLKTDADAVFDATVVIEAKDIAPQLTWGTNPGQVVAI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEEEHHCCCEEEECCCCCCEEEE
DQLVPNPLDATNPTVRTSIENALQYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAV
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECEECEEEEECCCCHHHHHHHHHHH
HAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNDDKLEV
HCCCCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure 
AKTLYEKVWDNHIVFAGEGEAPIIYVDRHLVHEVTSPQAFSGLKVAGRKLRAPEKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHCCCCCHHHEE
MDHNTSTTSASLDALSPMARTQVETLERNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP
ECCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGKVADGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDTTTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
EYLEGREFAPKGASWEQAVEAWSQLKTDADAVFDATVVIEAKDIAPQLTWGTNPGQVVAI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCHHHEEEEEEEEEHHCCCEEEECCCCCCEEEE
DQLVPNPLDATNPTVRTSIENALQYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAV
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECEECEEEEECCCCHHHHHHHHHHH
HAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFLEAGFEWRLPGCSMCLAMNDDKLEV
HCCCCCCCCCCEEEEECCCCCHHHHHHHCCHHHHHHHCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CHHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA