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The map label for this gene is leuB [H]

Identifier: 167622379

GI number: 167622379

Start: 516033

End: 517127

Strand: Direct

Name: leuB [H]

Synonym: Shal_0439

Alternate gene names: 167622379

Gene position: 516033-517127 (Clockwise)

Preceding gene: 167622378

Following gene: 167622380

Centisome position: 9.87

GC content: 48.68

Gene sequence:

>1095_bases
ATGAGTTATCAGATTGCAGTACTGGCGGGAGATGGCATAGGGCCAGAAGTGATGGTTGAAGCGCGCAAAGTGCTAAAGGC
AACAGAAGAGCGTTTTGCACTGTCTATTGAATATACCGAGTACGATGTGGGTGGCGCGGCTATCGATAATCATGGTTGCC
CGCTACCTGATGCCACGCTTAAAGGGTGTGAAGCGGCCGATGCGATTTTATTTGGCTCTGTTGGCGGCCCGAAGTGGGAG
CACCTTGCGCCTAACGATCAACCTGAGCGCGGTGCACTACTTCCTCTTCGTGGTCACTTTGAGTTGTTTTGTAATATGCG
CCCGGCAAAGCTACATGCGGGTCTTGAGCATATGTCACCACTACGCAGCGACATCTCTTCTCGTGGTTTCGATGTGCTTT
GTGTGCGTGAGCTAACCAGTGGTATTTACTTTGGTAAGCCAAAAGGTCGCATAGGAGAAGGTGAGAATGAAGAAGCGTTC
GATACTATGCGCTATAGCCGCAAAGAGATCAGACGTATCGCAAAAATCGCCTTCGAGACTGCGCAAGGTCGCCGTAAAAA
AGTCACTTCAGTCGATAAAGCCAATGTACTCGCTTGTTCAGTTTTATGGCGAGAAGTGGTTGAAGAGGTTGCTAAAGACT
TTCCAGATGTCGAGCTCGAGCATATCTATATCGATAACGCGACGATGCAGTTACTACGTCGTCCAGATGAATTTGATGTC
ATGCTGTGCTCTAACCTTTTCGGCGACATCATCTCAGATGAAATTGCCATGCTAACAGGCTCTATGGGTTTGCTTTCTTC
TATCAGTATGAATAGCGCAGGCTTTGGTTTATATGAGCCTGCGGGTGGTAGTGCGCCTGATATTGCCGGTCAAGGCATTG
CTAACCCTGTGGCGCAAATCCTTTCTGCAGCGCTATTACTACGTCATAGCCTCAAGCAGGAAGAGGCGGCGACAGTAATA
GAGAATGCAGTCGGTAAAGCCTTGAGCGATGGTTATCTAACGGGTGAGCTACTCGCAATTGAAGAGCGCCATAATGCAAA
ATCAACCAGTGAGATGGGTGACTATATTGCCAAGCTTGTGCGAGAAGGAGTGTAA

Upstream 100 bases:

>100_bases
ACAAGAGTTCGGCAGCGTATAAGCTTGCCCATTTAATGTCACCCAATACTTATAAATTGAAAATTAAATTAAAGAGCAAA
TTACAGGAGTTAGCTGACGT

Downstream 100 bases:

>100_bases
CCATGGCGAAGACATTGTATGAAAAGGTATGGGATAACCATATTGTGTTTGCTGGCGAAGGTGAGGCGCCAATCATCTAT
GTAGATAGGCATTTAGTGCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYQIAVLAGDGIGPEVMVEARKVLKATEERFALSIEYTEYDVGGAAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
HLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVLCVRELTSGIYFGKPKGRIGEGENEEAF
DTMRYSRKEIRRIAKIAFETAQGRRKKVTSVDKANVLACSVLWREVVEEVAKDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSAGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKQEEAATVI
ENAVGKALSDGYLTGELLAIEERHNAKSTSEMGDYIAKLVREGV

Sequences:

>Translated_364_residues
MSYQIAVLAGDGIGPEVMVEARKVLKATEERFALSIEYTEYDVGGAAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWE
HLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVLCVRELTSGIYFGKPKGRIGEGENEEAF
DTMRYSRKEIRRIAKIAFETAQGRRKKVTSVDKANVLACSVLWREVVEEVAKDFPDVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSAGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKQEEAATVI
ENAVGKALSDGYLTGELLAIEERHNAKSTSEMGDYIAKLVREGV
>Mature_363_residues
SYQIAVLAGDGIGPEVMVEARKVLKATEERFALSIEYTEYDVGGAAIDNHGCPLPDATLKGCEAADAILFGSVGGPKWEH
LAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVLCVRELTSGIYFGKPKGRIGEGENEEAFD
TMRYSRKEIRRIAKIAFETAQGRRKKVTSVDKANVLACSVLWREVVEEVAKDFPDVELEHIYIDNATMQLLRRPDEFDVM
LCSNLFGDIISDEIAMLTGSMGLLSSISMNSAGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKQEEAATVIE
NAVGKALSDGYLTGELLAIEERHNAKSTSEMGDYIAKLVREGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=31.547619047619, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=31.437125748503, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI4758582, Length=332, Percent_Identity=29.5180722891566, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI28178838, Length=339, Percent_Identity=28.6135693215339, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=31.437125748503, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI28178819, Length=162, Percent_Identity=39.5061728395062, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=67.7685950413223, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1788101, Length=370, Percent_Identity=35.6756756756757, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=24.6268656716418, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=31.2865497076023, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=29.940119760479, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.9659090909091, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=336, Percent_Identity=30.3571428571429, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=42.4324324324324, Blast_Score=258, Evalue=8e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=347, Percent_Identity=34.5821325648415, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=335, Percent_Identity=30.4477611940298, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=28.9017341040462, Blast_Score=123, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=33.9181286549708, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=33.9181286549708, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI161078635, Length=331, Percent_Identity=30.2114803625378, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078637, Length=333, Percent_Identity=30.03003003003, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078633, Length=333, Percent_Identity=30.03003003003, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650122, Length=333, Percent_Identity=30.03003003003, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078639, Length=327, Percent_Identity=30.5810397553517, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=29.3413173652695, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281362242, Length=334, Percent_Identity=27.2455089820359, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=334, Percent_Identity=27.2455089820359, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=333, Percent_Identity=25.2252252252252, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39530; Mature: 39398

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQIAVLAGDGIGPEVMVEARKVLKATEERFALSIEYTEYDVGGAAIDNHGCPLPDATL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEHLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRSDISSRGFDVLCVRELTSGIYFGKPKGRIGEGENEEAFDTMRYSRKEIRRIAKIAFET
HHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQGRRKKVTSVDKANVLACSVLWREVVEEVAKDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSAGFGLYEPAGGSAPDIAGQGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LSAALLLRHSLKQEEAATVIENAVGKALSDGYLTGELLAIEERHNAKSTSEMGDYIAKLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
REGV
HHCC
>Mature Secondary Structure 
SYQIAVLAGDGIGPEVMVEARKVLKATEERFALSIEYTEYDVGGAAIDNHGCPLPDATL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCEECCCCCCCCCHHH
KGCEAADAILFGSVGGPKWEHLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSP
CCCCHHCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRSDISSRGFDVLCVRELTSGIYFGKPKGRIGEGENEEAFDTMRYSRKEIRRIAKIAFET
HHHHHHHCCCEEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQGRRKKVTSVDKANVLACSVLWREVVEEVAKDFPDVELEHIYIDNATMQLLRRPDEFDV
HCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSAGFGLYEPAGGSAPDIAGQGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LSAALLLRHSLKQEEAATVIENAVGKALSDGYLTGELLAIEERHNAKSTSEMGDYIAKLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHH
REGV
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]