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The map label for this gene is menH [H]

Identifier: 167622237

GI number: 167622237

Start: 355431

End: 356282

Strand: Reverse

Name: menH [H]

Synonym: Shal_0296

Alternate gene names: 167622237

Gene position: 356282-355431 (Counterclockwise)

Preceding gene: 167622238

Following gene: 167622236

Centisome position: 6.82

GC content: 50.0

Gene sequence:

>852_bases
ATGCTGAGCTATCGCTGCAGAGGTCAAATAGGATCACCAGCCGTGGTACTGCTACACGGCTTTTTAGGCAGCAAAGAGGA
TTGGAATGAAATCATCGAGCAGTTAGCCGATGATTTCTTCTGCATCAGCATCGATCTTCCCGGCCACGGCGGTCATTGTA
GTCATAACAGCCATGGCGATACCACACAGGCCAGCGGTAACAATCAGCTCGATTTCACGAGCCAGAGTCTCGAGTTACCG
ACGCCGGGGTTTAACCGCTGCGCCGAGCTTATTCACACCACGCTCGAACAACTTAATATCAGTCAATATCACTTAGTGGG
TTATTCACTTGGTGGCCGTATCGCGCTGCATTTAGCGCGTCTCTACCCACAGGCATTGCTAAGCCTTTGCCTAGAGTCGT
GTCATCCTGGATTGCAAAATCAAGCCGATAGAGTCGCTCGTAAAGCCAATGACTTAGCTTGGGCGAGTAAACTAGAGACC
CTATCTATCAGGGATTTTTTAAGCCTTTGGTATCAGCAAGCGGTATTTAGCGACTTAACCGAAGCGGAGCGGGCAAAGCT
TATCGACAAACGCGCCGATAACCGCCCTTTGCAGCTACTAAATTGCTACCTCGCAACCTCCTTAGCCGAGCAGCAAGACC
TTTGGGATACCCCGAGCTGCCTACCTGTTGACTGCCACTTTATTGCCGGTCATCAAGACCATAAATTTATGGCGTTAGCC
AAACGCTGGCAGCAGCAACAGCCACTATCACTACACTGCATCGAAGCCAGTGGCCATAACGTACATCTGGCAGCTCCCGA
GGCTTTTGCAGAGCAACTCAAAACCATTTTTGCATCGAACTCTAACTTCTAA

Upstream 100 bases:

>100_bases
CCATGACCTACTCGGGTCCCTCTGTCATTGAGGTCACGGTTGCGCAGAACCAAGCCAGTGAGCAGATAGCTCAGATATCA
AGCTGGGTAAAACAGAGCTA

Downstream 100 bases:

>100_bases
CTAGACAGTGAGCACTCCATGACCGCGACTCTACAGCAAGCAAAGCCATTAACCTTAGCTAGGTTAGGTCTGTATCAGTT
TGCTATCGATTTAGATAAGC

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MLSYRCRGQIGSPAVVLLHGFLGSKEDWNEIIEQLADDFFCISIDLPGHGGHCSHNSHGDTTQASGNNQLDFTSQSLELP
TPGFNRCAELIHTTLEQLNISQYHLVGYSLGGRIALHLARLYPQALLSLCLESCHPGLQNQADRVARKANDLAWASKLET
LSIRDFLSLWYQQAVFSDLTEAERAKLIDKRADNRPLQLLNCYLATSLAEQQDLWDTPSCLPVDCHFIAGHQDHKFMALA
KRWQQQQPLSLHCIEASGHNVHLAAPEAFAEQLKTIFASNSNF

Sequences:

>Translated_283_residues
MLSYRCRGQIGSPAVVLLHGFLGSKEDWNEIIEQLADDFFCISIDLPGHGGHCSHNSHGDTTQASGNNQLDFTSQSLELP
TPGFNRCAELIHTTLEQLNISQYHLVGYSLGGRIALHLARLYPQALLSLCLESCHPGLQNQADRVARKANDLAWASKLET
LSIRDFLSLWYQQAVFSDLTEAERAKLIDKRADNRPLQLLNCYLATSLAEQQDLWDTPSCLPVDCHFIAGHQDHKFMALA
KRWQQQQPLSLHCIEASGHNVHLAAPEAFAEQLKTIFASNSNF
>Mature_283_residues
MLSYRCRGQIGSPAVVLLHGFLGSKEDWNEIIEQLADDFFCISIDLPGHGGHCSHNSHGDTTQASGNNQLDFTSQSLELP
TPGFNRCAELIHTTLEQLNISQYHLVGYSLGGRIALHLARLYPQALLSLCLESCHPGLQNQADRVARKANDLAWASKLET
LSIRDFLSLWYQQAVFSDLTEAERAKLIDKRADNRPLQLLNCYLATSLAEQQDLWDTPSCLPVDCHFIAGHQDHKFMALA
KRWQQQQPLSLHCIEASGHNVHLAAPEAFAEQLKTIFASNSNF

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Homo sapiens, GI189027141, Length=164, Percent_Identity=29.8780487804878, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1788598, Length=270, Percent_Identity=33.3333333333333, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 31588; Mature: 31588

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSYRCRGQIGSPAVVLLHGFLGSKEDWNEIIEQLADDFFCISIDLPGHGGHCSHNSHGD
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
TTQASGNNQLDFTSQSLELPTPGFNRCAELIHTTLEQLNISQYHLVGYSLGGRIALHLAR
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
LYPQALLSLCLESCHPGLQNQADRVARKANDLAWASKLETLSIRDFLSLWYQQAVFSDLT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAERAKLIDKRADNRPLQLLNCYLATSLAEQQDLWDTPSCLPVDCHFIAGHQDHKFMALA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHH
KRWQQQQPLSLHCIEASGHNVHLAAPEAFAEQLKTIFASNSNF
HHHHHCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLSYRCRGQIGSPAVVLLHGFLGSKEDWNEIIEQLADDFFCISIDLPGHGGHCSHNSHGD
CCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCC
TTQASGNNQLDFTSQSLELPTPGFNRCAELIHTTLEQLNISQYHLVGYSLGGRIALHLAR
CCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
LYPQALLSLCLESCHPGLQNQADRVARKANDLAWASKLETLSIRDFLSLWYQQAVFSDLT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAERAKLIDKRADNRPLQLLNCYLATSLAEQQDLWDTPSCLPVDCHFIAGHQDHKFMALA
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHH
KRWQQQQPLSLHCIEASGHNVHLAAPEAFAEQLKTIFASNSNF
HHHHHCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA