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The map label for this gene is menD

Identifier: 167622238

GI number: 167622238

Start: 356282

End: 357991

Strand: Reverse

Name: menD

Synonym: Shal_0297

Alternate gene names: 167622238

Gene position: 357991-356282 (Counterclockwise)

Preceding gene: 167622240

Following gene: 167622237

Centisome position: 6.85

GC content: 48.77

Gene sequence:

>1710_bases
ATGCAAACCGAACATACAGCTGAACTGAACCTGCTTTGGGGAACGTTAATCTTAGAAGAACTGGCCCGTCTTGGTGTCAA
ACACGTCTGTATGGCGCCAGGTTCCCGCTCCACTCCATTAACTCTGGCAGCGGCAAAACAGTCGAAGCTTAGCCAACATA
TTCATTTTGATGAAAGAGGCCTGGGCTTTATGGCACTCGGCTTAGCTAAAGCGAGCCAAGCTCCAGTTGCGATTATTACC
ACTTCAGGGACGGCAGTAGCCAACCTTTACCCTGCGATTATTGAAGCTTGGCTCACTCACGTTCCTTTAATCGTGTTATC
TGGCGATAGACCACCAGAGCTCATCGACTGTGGTGCTAACCAAGCCATTATTCAGCCTGCTATTTTCGCTCAGTATGCCA
AACAGCTCAATTTGCCAACCCCCGACTTGAACATAGCGCCCCAAGCACTACTGAGCCTGTTAGATGACGCTGTGTGTAAT
CAATCACAACCCGTGCATATCAACTGCATGTACCGTGAGCCTCTATATCCAAATGAGCCTAGTGTCGACTTTTCACGTTA
TTTAGCGCCCATTGCAAAGTGGCAGCAACAAACTAAGCCTTATAGCCAGTTCGCCACAATGAATTCAGGTCAGATGCCGA
CATCCGATGCCCTCGCACGTTTTGTTCACGGTAAAGGGGTGATTGTTGCGGCAACACTAGCGCCTGAGCAGCAGAGCGAA
GAGCTTATTGTGCTTGCACAAAAGCTTGGCTGGCCCATTGTCACCGATGCTCAGTCACAGCTCAGACAGCACGGCGGCGC
AATCGGTAATATCGATCAATTGCTGCAGCAGCCAAAATCTAAGGCGTTATTAGCCCAAGCAGAAAAAGTGCTTGTGTTTG
GCGGACGTATTCTGTCAAAACGCTTGATTAGTTATCTCAATGATCAAGATTGGAAAGATTACTGGCAAGTGCTCCCGCAG
CAACAGCGACTTGACCCAAGCAATAAACCTAAACAGGTTTGGATTGCTCCGGCGCATCAGTTTGCTGCACTAGCATGGCC
AAGATCATCACAGGCCAATTGGGCACTAAACTTAGTACAGTTTAACGATGAGCTAGAAACCCTCTATCAGCAGCAAATTG
ATCATGCTGAATTTGGTGAAGCGCAAGCCATTCGCGCTATTGCTAAGCGCCAGACCAATGAGCAACAGCTGTTTATCGGT
AATAGTTTGCCAATAAGACTGTATGATATGTTTGCGCCAATCACTACCTCTAGCGCCAATGTATTTACTAATCGCGGCGC
ATCGGGCATCGATGGCTTACTGGCAACGGCATGTGGCGTTGCCATAGCGGGGGCGAAACCAACGACACTGATCATTGGTG
ATATCTCGGCTTTACATGATCTTAATTCGTTGGCGATTGCACGCACGGTAACCAGCCCTTTCGTGATCGTTATCCTCAAC
AACGATGGCGGTAATATCTTTAATTTGCTGCCCGTGCCGAATGAACAACTGAGAAGCGACTACTACCGCTTGTCACACGG
ACTCGAATTTGGCTTCGGCGCGGCGATGTTTGGCCTAGCCTACAATCAGGTTGACTCGTTAAGCGACTTTATTGAGTCCT
ATGAGTACGCCATGACCTACTCGGGTCCCTCTGTCATTGAGGTCACGGTTGCGCAGAACCAAGCCAGTGAGCAGATAGCT
CAGATATCAAGCTGGGTAAAACAGAGCTAA

Upstream 100 bases:

>100_bases
CTGACAAACAAAGCGCTTCATAAAGAAAACATAAGAGCGATCATACTCCTTTAGGCTAAAGCAAGTTAAACTAAGGCTAT
CAATAAGATGGTACTTATCT

Downstream 100 bases:

>100_bases
TGCTGAGCTATCGCTGCAGAGGTCAAATAGGATCACCAGCCGTGGTACTGCTACACGGCTTTTTAGGCAGCAAAGAGGAT
TGGAATGAAATCATCGAGCA

Product: 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQSKLSQHIHFDERGLGFMALGLAKASQAPVAIIT
TSGTAVANLYPAIIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQLNLPTPDLNIAPQALLSLLDDAVCN
QSQPVHINCMYREPLYPNEPSVDFSRYLAPIAKWQQQTKPYSQFATMNSGQMPTSDALARFVHGKGVIVAATLAPEQQSE
ELIVLAQKLGWPIVTDAQSQLRQHGGAIGNIDQLLQQPKSKALLAQAEKVLVFGGRILSKRLISYLNDQDWKDYWQVLPQ
QQRLDPSNKPKQVWIAPAHQFAALAWPRSSQANWALNLVQFNDELETLYQQQIDHAEFGEAQAIRAIAKRQTNEQQLFIG
NSLPIRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAIAGAKPTTLIIGDISALHDLNSLAIARTVTSPFVIVILN
NDGGNIFNLLPVPNEQLRSDYYRLSHGLEFGFGAAMFGLAYNQVDSLSDFIESYEYAMTYSGPSVIEVTVAQNQASEQIA
QISSWVKQS

Sequences:

>Translated_569_residues
MQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQSKLSQHIHFDERGLGFMALGLAKASQAPVAIIT
TSGTAVANLYPAIIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQLNLPTPDLNIAPQALLSLLDDAVCN
QSQPVHINCMYREPLYPNEPSVDFSRYLAPIAKWQQQTKPYSQFATMNSGQMPTSDALARFVHGKGVIVAATLAPEQQSE
ELIVLAQKLGWPIVTDAQSQLRQHGGAIGNIDQLLQQPKSKALLAQAEKVLVFGGRILSKRLISYLNDQDWKDYWQVLPQ
QQRLDPSNKPKQVWIAPAHQFAALAWPRSSQANWALNLVQFNDELETLYQQQIDHAEFGEAQAIRAIAKRQTNEQQLFIG
NSLPIRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAIAGAKPTTLIIGDISALHDLNSLAIARTVTSPFVIVILN
NDGGNIFNLLPVPNEQLRSDYYRLSHGLEFGFGAAMFGLAYNQVDSLSDFIESYEYAMTYSGPSVIEVTVAQNQASEQIA
QISSWVKQS
>Mature_569_residues
MQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQSKLSQHIHFDERGLGFMALGLAKASQAPVAIIT
TSGTAVANLYPAIIEAWLTHVPLIVLSGDRPPELIDCGANQAIIQPAIFAQYAKQLNLPTPDLNIAPQALLSLLDDAVCN
QSQPVHINCMYREPLYPNEPSVDFSRYLAPIAKWQQQTKPYSQFATMNSGQMPTSDALARFVHGKGVIVAATLAPEQQSE
ELIVLAQKLGWPIVTDAQSQLRQHGGAIGNIDQLLQQPKSKALLAQAEKVLVFGGRILSKRLISYLNDQDWKDYWQVLPQ
QQRLDPSNKPKQVWIAPAHQFAALAWPRSSQANWALNLVQFNDELETLYQQQIDHAEFGEAQAIRAIAKRQTNEQQLFIG
NSLPIRLYDMFAPITTSSANVFTNRGASGIDGLLATACGVAIAGAKPTTLIIGDISALHDLNSLAIARTVTSPFVIVILN
NDGGNIFNLLPVPNEQLRSDYYRLSHGLEFGFGAAMFGLAYNQVDSLSDFIESYEYAMTYSGPSVIEVTVAQNQASEQIA
QISSWVKQS

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=556, Percent_Identity=40.2877697841727, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_SHEHH (B0TNX0)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672532.1
- ProteinModelPortal:   B0TNX0
- GeneID:   5904512
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0297
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   PRK07449
- BioCyc:   SHAL458817:SHAL_0297-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 62285; Mature: 62285

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQSKLSQHIHFDERG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCCC
LGFMALGLAKASQAPVAIITTSGTAVANLYPAIIEAWLTHVPLIVLSGDRPPELIDCGAN
CCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHEECCCC
QAIIQPAIFAQYAKQLNLPTPDLNIAPQALLSLLDDAVCNQSQPVHINCMYREPLYPNEP
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
SVDFSRYLAPIAKWQQQTKPYSQFATMNSGQMPTSDALARFVHGKGVIVAATLAPEQQSE
CCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCC
ELIVLAQKLGWPIVTDAQSQLRQHGGAIGNIDQLLQQPKSKALLAQAEKVLVFGGRILSK
HHEEEHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCEEEEECHHHHHH
RLISYLNDQDWKDYWQVLPQQQRLDPSNKPKQVWIAPAHQFAALAWPRSSQANWALNLVQ
HHHHHHCCCCHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHHEEECCCCCCCCEEEEEEE
FNDELETLYQQQIDHAEFGEAQAIRAIAKRQTNEQQLFIGNSLPIRLYDMFAPITTSSAN
ECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHCCCCCCCCC
VFTNRGASGIDGLLATACGVAIAGAKPTTLIIGDISALHDLNSLAIARTVTSPFVIVILN
CEECCCCCCHHHHHHHHHHHEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEE
NDGGNIFNLLPVPNEQLRSDYYRLSHGLEFGFGAAMFGLAYNQVDSLSDFIESYEYAMTY
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
SGPSVIEVTVAQNQASEQIAQISSWVKQS
CCCCEEEEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTEHTAELNLLWGTLILEELARLGVKHVCMAPGSRSTPLTLAAAKQSKLSQHIHFDERG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCCC
LGFMALGLAKASQAPVAIITTSGTAVANLYPAIIEAWLTHVPLIVLSGDRPPELIDCGAN
CCEEEEHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCEEEECCCCCCHHEECCCC
QAIIQPAIFAQYAKQLNLPTPDLNIAPQALLSLLDDAVCNQSQPVHINCMYREPLYPNEP
CHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCC
SVDFSRYLAPIAKWQQQTKPYSQFATMNSGQMPTSDALARFVHGKGVIVAATLAPEQQSE
CCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCC
ELIVLAQKLGWPIVTDAQSQLRQHGGAIGNIDQLLQQPKSKALLAQAEKVLVFGGRILSK
HHEEEHHHHCCCEEECHHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHCEEEEECHHHHHH
RLISYLNDQDWKDYWQVLPQQQRLDPSNKPKQVWIAPAHQFAALAWPRSSQANWALNLVQ
HHHHHHCCCCHHHHHHHCCHHHCCCCCCCCCEEEEECCHHHHEEECCCCCCCCEEEEEEE
FNDELETLYQQQIDHAEFGEAQAIRAIAKRQTNEQQLFIGNSLPIRLYDMFAPITTSSAN
ECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEEHHCCCCCCCCC
VFTNRGASGIDGLLATACGVAIAGAKPTTLIIGDISALHDLNSLAIARTVTSPFVIVILN
CEECCCCCCHHHHHHHHHHHEEECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEEE
NDGGNIFNLLPVPNEQLRSDYYRLSHGLEFGFGAAMFGLAYNQVDSLSDFIESYEYAMTY
CCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
SGPSVIEVTVAQNQASEQIAQISSWVKQS
CCCCEEEEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA