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The map label for this gene is cyoE

Identifier: 167622192

GI number: 167622192

Start: 301349

End: 302254

Strand: Reverse

Name: cyoE

Synonym: Shal_0251

Alternate gene names: 167622192

Gene position: 302254-301349 (Counterclockwise)

Preceding gene: 167622193

Following gene: 167622191

Centisome position: 5.78

GC content: 47.24

Gene sequence:

>906_bases
ATGCCAAAACCCATCGCACTCTCAAGAAACCCATCAACACCACTGTTTCAATGGCGTGCTTATTACGAAATGACTAAGCC
TAAGGTCGTGGCACTCATGCTACTCACCGTATTAGTCGGTATGTGCTTAGCCGTACCTGGAGTCGTACCTATAGTCCCTT
TAATTGCTGGCATGGCAGGAATTGGCATGATGGCAGGCTCGGCGGCGGCCTTTAATCATCTCATCGATCGTCGTATCGAT
GGCTTAATGGCGCGTACCTATAATCGTCCACTGCCCAAAGGGCGAGTATCGATAGCAAAAGCACTAACCTTTTCATGTAG
TCTTGGGATCCTTGGCTTTGTGGTGCTGTATGTCCTAGTCAACCCACTAACAGCATGGCTCACCTTTGCGAGTTTGATAG
GTTATGCCGTGGTTTATACCGCTTACTTAAAGCGTGCGACACCACAAAATATTGTAGTTGGCGGCTTAGCGGGTGCCATG
CCGCCACTACTTGGCTGGACAGCGGTAACAGGCGAGTTTCACGGTAACGCGCTGCTACTCGTCATCATCATTTTTGCCTG
GACGCCACCGCATTTTTGGGCGTTGGCTATTCATCGAAGAGTCGAATATGCCAAGGTCGACATCCCTATGCTGCCAGTGA
CTCATGGTGTCGAGTTCACCAAAACCTGTATTTTCCTCTATACCATCTTGTTAGCTATCGCTTGCTTATTGCCAGTATTA
GTAGGCATGTGTGGCCCGGTTTATTTGGTGAGTTCAACACTACTCAGCGTCACTTTTATCTATAAAGCTTGGCAACTCAA
GTTCCATGAAGAACCCGGCATGGCGATGAAGCTATTTAAGTTTTCAATCTATCACCTGATGCTACTGTTTATTGCTCTAT
TGGTTGATCATTACTTGTGGCTATAA

Upstream 100 bases:

>100_bases
GGTATCGCCGTTTCCCATAATGGCGGCGCAGCGCTATTACTACTGACATTAGTCTTTATTAATTACGCCCTATGGCGCAA
AGCATAGCGAGGTTTAACTT

Downstream 100 bases:

>100_bases
ATCTATGGTTATAAATCTATGCCTATAAAAATTATTAGTATTAGCTGTCAGGTATTGGGCGCACTTCGTCATACGCTAGG
AGATAAAAGATGAGGCTCAC

Product: protoheme IX farnesyltransferase

Products: NA

Alternate protein names: Heme B farnesyltransferase; Heme O synthase

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MPKPIALSRNPSTPLFQWRAYYEMTKPKVVALMLLTVLVGMCLAVPGVVPIVPLIAGMAGIGMMAGSAAAFNHLIDRRID
GLMARTYNRPLPKGRVSIAKALTFSCSLGILGFVVLYVLVNPLTAWLTFASLIGYAVVYTAYLKRATPQNIVVGGLAGAM
PPLLGWTAVTGEFHGNALLLVIIIFAWTPPHFWALAIHRRVEYAKVDIPMLPVTHGVEFTKTCIFLYTILLAIACLLPVL
VGMCGPVYLVSSTLLSVTFIYKAWQLKFHEEPGMAMKLFKFSIYHLMLLFIALLVDHYLWL

Sequences:

>Translated_301_residues
MPKPIALSRNPSTPLFQWRAYYEMTKPKVVALMLLTVLVGMCLAVPGVVPIVPLIAGMAGIGMMAGSAAAFNHLIDRRID
GLMARTYNRPLPKGRVSIAKALTFSCSLGILGFVVLYVLVNPLTAWLTFASLIGYAVVYTAYLKRATPQNIVVGGLAGAM
PPLLGWTAVTGEFHGNALLLVIIIFAWTPPHFWALAIHRRVEYAKVDIPMLPVTHGVEFTKTCIFLYTILLAIACLLPVL
VGMCGPVYLVSSTLLSVTFIYKAWQLKFHEEPGMAMKLFKFSIYHLMLLFIALLVDHYLWL
>Mature_300_residues
PKPIALSRNPSTPLFQWRAYYEMTKPKVVALMLLTVLVGMCLAVPGVVPIVPLIAGMAGIGMMAGSAAAFNHLIDRRIDG
LMARTYNRPLPKGRVSIAKALTFSCSLGILGFVVLYVLVNPLTAWLTFASLIGYAVVYTAYLKRATPQNIVVGGLAGAMP
PLLGWTAVTGEFHGNALLLVIIIFAWTPPHFWALAIHRRVEYAKVDIPMLPVTHGVEFTKTCIFLYTILLAIACLLPVLV
GMCGPVYLVSSTLLSVTFIYKAWQLKFHEEPGMAMKLFKFSIYHLMLLFIALLVDHYLWL

Specific function: Converts heme B (protoheme IX) to heme O by substitution of the vinyl group on carbon 2 of heme B porphyrin ring with a hydroxyethyl farnesyl side group

COG id: COG0109

COG function: function code O; Polyprenyltransferase (cytochrome oxidase assembly factor)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiA prenyltransferase family. Protoheme IX farnesyltransferase subfamily

Homologues:

Organism=Homo sapiens, GI17921982, Length=193, Percent_Identity=35.7512953367876, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI1786631, Length=280, Percent_Identity=37.8571428571429, Blast_Score=175, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71998235, Length=264, Percent_Identity=29.9242424242424, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325085, Length=263, Percent_Identity=27.7566539923954, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19921064, Length=302, Percent_Identity=30.1324503311258, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYOE_SHEHH (B0TNS4)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001672486.1
- GeneID:   5906529
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_0251
- HOGENOM:   HBG656003
- OMA:   LLKPRIM
- ProtClustDB:   PRK04375
- BioCyc:   SHAL458817:SHAL_0251-MONOMER
- HAMAP:   MF_00154
- InterPro:   IPR006369
- InterPro:   IPR000537
- PANTHER:   PTHR11048:SF3
- TIGRFAMs:   TIGR01473

Pfam domain/function: PF01040 UbiA

EC number: 2.5.1.-

Molecular weight: Translated: 33180; Mature: 33049

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00943 UBIA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20f3ae80)-; HASH(0x24333d34)-; HASH(0x2462a698)-; HASH(0x259fba98)-; HASH(0x25e4e30c)-; HASH(0x25f1ee50)-; HASH(0x2640bf8c)-; HASH(0x257bdcb4)-; HASH(0x2563580c)-;

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKPIALSRNPSTPLFQWRAYYEMTKPKVVALMLLTVLVGMCLAVPGVVPIVPLIAGMAG
CCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
IGMMAGSAAAFNHLIDRRIDGLMARTYNRPLPKGRVSIAKALTFSCSLGILGFVVLYVLV
HHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
NPLTAWLTFASLIGYAVVYTAYLKRATPQNIVVGGLAGAMPPLLGWTAVTGEFHGNALLL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHCHHHHHHHHCCCCCCCCHHHH
VIIIFAWTPPHFWALAIHRRVEYAKVDIPMLPVTHGVEFTKTCIFLYTILLAIACLLPVL
HHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VGMCGPVYLVSSTLLSVTFIYKAWQLKFHEEPGMAMKLFKFSIYHLMLLFIALLVDHYLW
HHHCCHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
L
H
>Mature Secondary Structure 
PKPIALSRNPSTPLFQWRAYYEMTKPKVVALMLLTVLVGMCLAVPGVVPIVPLIAGMAG
CCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH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H

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA