Click here to switch to the map view.

The map label for this gene is aes [H]

Identifier: 167622168

GI number: 167622168

Start: 274046

End: 274984

Strand: Direct

Name: aes [H]

Synonym: Shal_0227

Alternate gene names: 167622168

Gene position: 274046-274984 (Clockwise)

Preceding gene: 167622167

Following gene: 167622169

Centisome position: 5.24

GC content: 49.52

Gene sequence:

>939_bases
ATGCCGTTAGATCCAAAAGTCGCACTGTATTTGCAACAAGTGCGTGATTCTGGCGCACAAGCCTATGAAGACATGACCCC
CGCAGAGTCTAGACGCCTCGATATGAATGAGCTGACGAAGACGAAAGCCGATCGAGTGTTAGAGCCCGTGGCCGCGATAG
AGCATAGCTTTATTCCTGGACCCACTGCAGATCTGCCCATTCGGATCTACCGTCCTAAAGGTGACACGAGTGAGTTACAG
CCGGCGATTATCTTTATTCATGGCAGCGGTTGGGTGGTATCGAACATAGAGACCAATGACCATTTTAGCCGTGCCCTCGC
TAATCGCACCGGATCGGTAGTGATCGCGATTAATTATCAAAAGGCACCTGAGCACAAGTTTCCCATCCCAATGGATGACT
GCTATGCATCGACATTGTGGATCTTTGAGCATGCGACTTTGCTGGGATTAGACTCGAACCGTATCGGCATCTTAGGTGAC
AGTGCCGGTGGTAATTTAGCAGCGGCGGTGACGCTAAGGCTGCGTGACGAACAGGGGCCAAAATTGGCTTACCAAGTACT
CGTTTATCCAGCGGTGCAATATGGTTGGCAAACGCCATCGGCTATCGCTCATGCAGAAGGTTATCTATTGCAACAGGCCA
GTATGAAGTATTACTGGGGCCACTACCTGCGTAGCGAAGCCGACGCACAAAATCCCTATTGTTCACCGCTGAATGCCCTG
AGCCACAAAGATCTGCCGCCAACACTTATCTATACCGCAGAGTTTGATCCATTATGCGATGACGGCTATCTCTATGCCCG
AGAATTACAAGGCAGTGGTGTTACAGTCAAATACCGCTGTTTTGACGGCGTGATCCATGGGTTTATTAAGATGCTTGGGG
TGTTTGATCAGGCCGATGAGTTTCTAGATGAACTTAAGCAAGATCTGTTAGAGATCTAG

Upstream 100 bases:

>100_bases
AGCACCTCAGGCAGCACGCTTAATAATTGAAACTATTAATTGAAACTATAGGCAGCTGAACAACCATGGTTGGCAGACTG
CAATTGAGAAGGAAAGCGGT

Downstream 100 bases:

>100_bases
GTGTGATTAGTTAAAAACATAACTTGGGAATGCTCACGAAACAAACGGTTTCGTGAGCTTGTTCGAAGAATCATCAACTT
GTTCCTCTAGAAAAATTAAT

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MPLDPKVALYLQQVRDSGAQAYEDMTPAESRRLDMNELTKTKADRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQ
PAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHATLLGLDSNRIGILGD
SAGGNLAAAVTLRLRDEQGPKLAYQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAQNPYCSPLNAL
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGVTVKYRCFDGVIHGFIKMLGVFDQADEFLDELKQDLLEI

Sequences:

>Translated_312_residues
MPLDPKVALYLQQVRDSGAQAYEDMTPAESRRLDMNELTKTKADRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQ
PAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHATLLGLDSNRIGILGD
SAGGNLAAAVTLRLRDEQGPKLAYQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAQNPYCSPLNAL
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGVTVKYRCFDGVIHGFIKMLGVFDQADEFLDELKQDLLEI
>Mature_311_residues
PLDPKVALYLQQVRDSGAQAYEDMTPAESRRLDMNELTKTKADRVLEPVAAIEHSFIPGPTADLPIRIYRPKGDTSELQP
AIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQKAPEHKFPIPMDDCYASTLWIFEHATLLGLDSNRIGILGDS
AGGNLAAAVTLRLRDEQGPKLAYQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAQNPYCSPLNALS
HKDLPPTLIYTAEFDPLCDDGYLYARELQGSGVTVKYRCFDGVIHGFIKMLGVFDQADEFLDELKQDLLEI

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=195, Percent_Identity=34.8717948717949, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI157041239, Length=138, Percent_Identity=36.231884057971, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI206597554, Length=137, Percent_Identity=37.2262773722628, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI61966717, Length=161, Percent_Identity=31.6770186335404, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI68051721, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI157041237, Length=107, Percent_Identity=37.3831775700935, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI21328446, Length=91, Percent_Identity=37.3626373626374, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI226423947, Length=178, Percent_Identity=29.2134831460674, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=264, Percent_Identity=31.0606060606061, Blast_Score=121, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71996133, Length=308, Percent_Identity=30.1948051948052, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17540028, Length=314, Percent_Identity=26.7515923566879, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=264, Percent_Identity=31.0606060606061, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=36.1538461538462, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115533410, Length=137, Percent_Identity=27.7372262773723, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=137, Percent_Identity=27.7372262773723, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34782; Mature: 34651

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDPKVALYLQQVRDSGAQAYEDMTPAESRRLDMNELTKTKADRVLEPVAAIEHSFIPG
CCCCCHHHHHHHHHHHCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PTADLPIRIYRPKGDTSELQPAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQ
CCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEEECC
KAPEHKFPIPMDDCYASTLWIFEHATLLGLDSNRIGILGDSAGGNLAAAVTLRLRDEQGP
CCCCCCCCCCHHHHHHHHHHEEECEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
KLAYQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAQNPYCSPLNAL
CEEEHHEEHHHHHHCCCCCHHHHHHCCHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHH
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGVTVKYRCFDGVIHGFIKMLGVFDQADE
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
FLDELKQDLLEI
HHHHHHHHHHCC
>Mature Secondary Structure 
PLDPKVALYLQQVRDSGAQAYEDMTPAESRRLDMNELTKTKADRVLEPVAAIEHSFIPG
CCCCHHHHHHHHHHHCHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
PTADLPIRIYRPKGDTSELQPAIIFIHGSGWVVSNIETNDHFSRALANRTGSVVIAINYQ
CCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEECCCCHHHHHHHHCCCCCEEEEEECC
KAPEHKFPIPMDDCYASTLWIFEHATLLGLDSNRIGILGDSAGGNLAAAVTLRLRDEQGP
CCCCCCCCCCHHHHHHHHHHEEECEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCC
KLAYQVLVYPAVQYGWQTPSAIAHAEGYLLQQASMKYYWGHYLRSEADAQNPYCSPLNAL
CEEEHHEEHHHHHHCCCCCHHHHHHCCHHHHHHCHHHHHHHHHHCCCCCCCCCCCCHHHH
SHKDLPPTLIYTAEFDPLCDDGYLYARELQGSGVTVKYRCFDGVIHGFIKMLGVFDQADE
CCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
FLDELKQDLLEI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA