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The map label for this gene is acrA [H]

Identifier: 167622138

GI number: 167622138

Start: 232852

End: 234003

Strand: Direct

Name: acrA [H]

Synonym: Shal_0197

Alternate gene names: 167622138

Gene position: 232852-234003 (Clockwise)

Preceding gene: 167622137

Following gene: 167622139

Centisome position: 4.45

GC content: 46.53

Gene sequence:

>1152_bases
ATGCGGCAAATAGTGAAAATTGCCTCTGTTTTAAGTGTGGCGTTATGGATAAGTGGCTGTGAGCAAAAACCTGAACAGGG
GCCGCAACAGCGTGGACCAATGGAAGTGGGGGTTATAACTATTGCTGCTAAGCCTCAATCAATCATGGTTGAATTACCGG
GTCGTAGTAAGTCTTATTTAGAGGCTGAAGTGCGTCCACAGGTAAGCGGTATTATTATGGAACGTGGTTTTGTTGAAGGT
AGGGATGTCGAAAAAGGACAATCTTTATATCAGATCGAGCCATCAACCTACGAAGCAGCACTCGTTAGTGCTAAAGCCGA
TCTTAAGAGTGCGCAAGCGGGTCTCGTTTCGGCTAAAGCAAAGGCACTGCGTTTTGAAAAACTAGTCAAGCTTAACTCAA
TCAGTGAGCAAGATTATGATGAAGCGGATGCGGCTTACAAAGAAGCTCTAGCACGTGTAACGGTTGCTGAAGCGAATATT
AATACTGCTCAAATTAATCTAGAGTACACCAAGGTAACGGCGCCAATCTCTGGCCGTATCGGTAAGTCTGATGTGACTGC
TGGTGCACTTGTTACCGCAAATCAGAGCCAAACCTTGGCGACGATTCAGCAACTTGACCCAATGAATATCGATATCGCTC
AGTCAAGCACGCAGATGCTGCGTCTTAAGTCTCAGCTAAAGCGTGGTCAAATTGAGGCTGCGGCAAATGCCAATGTTGAG
CTGATTTTAGAAGACGGTACTCCGTACCATCATACTGGTGTGTTGCAGTTTGCTGAGGTGACGGTCAACGAAAATACCGG
TTCGGTGATTATCCGTGCAGAATTTCCAAACCCAGACGGTGTGTTGATGCCTGGCATGTATGTACGTGCAGTATTGAATA
CGGGTACCGATCCTGAAGCGATTCTTGTCCCACAAAAAGCCATCACTCGTAATACTAAGGGACAGGCTGTGGCTATGGTT
ATTGAAGATGGCAAGGTTGCAGCGCGCATCGTGACTACAGCTGAAGTGATTGACAATCAATGGCGTATTACCAGCGGCCT
TAAAGTAGGCGAACAGCTAATCGTTGAAGGCCTTCAAAAAATTCGTCCTGGAGCCCCTGTTAAAGCGGGACCTTTGGCCG
CTGATAAGAAACCAGAAACACAACAGAAATAG

Upstream 100 bases:

>100_bases
TAAATATTCGACAACAATTTATGCTTCCAAAGGTGGATTTGATGGCACTTTCTGCTTTCAAAAAATATTACCGAGTAGAT
GGCTTTTATTAGGAGTTCTC

Downstream 100 bases:

>100_bases
GATAGGGTTATGGCACGTTTCTTTATCGATCGCCCTATTTTTGCATGGGTGATCGCTATTATTGTGATGCTGGCAGGTGT
GCTCTCTATTGTGGGCCTAC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRQIVKIASVLSVALWISGCEQKPEQGPQQRGPMEVGVITIAAKPQSIMVELPGRSKSYLEAEVRPQVSGIIMERGFVEG
RDVEKGQSLYQIEPSTYEAALVSAKADLKSAQAGLVSAKAKALRFEKLVKLNSISEQDYDEADAAYKEALARVTVAEANI
NTAQINLEYTKVTAPISGRIGKSDVTAGALVTANQSQTLATIQQLDPMNIDIAQSSTQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYHHTGVLQFAEVTVNENTGSVIIRAEFPNPDGVLMPGMYVRAVLNTGTDPEAILVPQKAITRNTKGQAVAMV
IEDGKVAARIVTTAEVIDNQWRITSGLKVGEQLIVEGLQKIRPGAPVKAGPLAADKKPETQQK

Sequences:

>Translated_383_residues
MRQIVKIASVLSVALWISGCEQKPEQGPQQRGPMEVGVITIAAKPQSIMVELPGRSKSYLEAEVRPQVSGIIMERGFVEG
RDVEKGQSLYQIEPSTYEAALVSAKADLKSAQAGLVSAKAKALRFEKLVKLNSISEQDYDEADAAYKEALARVTVAEANI
NTAQINLEYTKVTAPISGRIGKSDVTAGALVTANQSQTLATIQQLDPMNIDIAQSSTQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYHHTGVLQFAEVTVNENTGSVIIRAEFPNPDGVLMPGMYVRAVLNTGTDPEAILVPQKAITRNTKGQAVAMV
IEDGKVAARIVTTAEVIDNQWRITSGLKVGEQLIVEGLQKIRPGAPVKAGPLAADKKPETQQK
>Mature_383_residues
MRQIVKIASVLSVALWISGCEQKPEQGPQQRGPMEVGVITIAAKPQSIMVELPGRSKSYLEAEVRPQVSGIIMERGFVEG
RDVEKGQSLYQIEPSTYEAALVSAKADLKSAQAGLVSAKAKALRFEKLVKLNSISEQDYDEADAAYKEALARVTVAEANI
NTAQINLEYTKVTAPISGRIGKSDVTAGALVTANQSQTLATIQQLDPMNIDIAQSSTQMLRLKSQLKRGQIEAAANANVE
LILEDGTPYHHTGVLQFAEVTVNENTGSVIIRAEFPNPDGVLMPGMYVRAVLNTGTDPEAILVPQKAITRNTKGQAVAMV
IEDGKVAARIVTTAEVIDNQWRITSGLKVGEQLIVEGLQKIRPGAPVKAGPLAADKKPETQQK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=49.7282608695652, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1789665, Length=362, Percent_Identity=48.3425414364641, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1789929, Length=362, Percent_Identity=44.7513812154696, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI87082036, Length=308, Percent_Identity=25.974025974026, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=24.3975903614458, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41173; Mature: 41173

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIVKIASVLSVALWISGCEQKPEQGPQQRGPMEVGVITIAAKPQSIMVELPGRSKSYL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEEEEEECCCEEEEECCCCCCCCE
EAEVRPQVSGIIMERGFVEGRDVEKGQSLYQIEPSTYEAALVSAKADLKSAQAGLVSAKA
EEECCCCHHEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALRFEKLVKLNSISEQDYDEADAAYKEALARVTVAEANINTAQINLEYTKVTAPISGRI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEECCCCCCC
GKSDVTAGALVTANQSQTLATIQQLDPMNIDIAQSSTQMLRLKSQLKRGQIEAAANANVE
CCCCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEECCCCEE
LILEDGTPYHHTGVLQFAEVTVNENTGSVIIRAEFPNPDGVLMPGMYVRAVLNTGTDPEA
EEEECCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEECCCHHHHHHHCCCCCCCE
ILVPQKAITRNTKGQAVAMVIEDGKVAARIVTTAEVIDNQWRITSGLKVGEQLIVEGLQK
EEECCHHHHCCCCCCEEEEEEECCCEEEEEEEEHHHHCCCEEEECCCHHHHHHHHHHHHH
IRPGAPVKAGPLAADKKPETQQK
HCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRQIVKIASVLSVALWISGCEQKPEQGPQQRGPMEVGVITIAAKPQSIMVELPGRSKSYL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCEEEEEEEEEECCCEEEEECCCCCCCCE
EAEVRPQVSGIIMERGFVEGRDVEKGQSLYQIEPSTYEAALVSAKADLKSAQAGLVSAKA
EEECCCCHHEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALRFEKLVKLNSISEQDYDEADAAYKEALARVTVAEANINTAQINLEYTKVTAPISGRI
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEECCCCEEEEEEEEEEEECCCCCCC
GKSDVTAGALVTANQSQTLATIQQLDPMNIDIAQSSTQMLRLKSQLKRGQIEAAANANVE
CCCCCCCCEEEECCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCEEEECCCCEE
LILEDGTPYHHTGVLQFAEVTVNENTGSVIIRAEFPNPDGVLMPGMYVRAVLNTGTDPEA
EEEECCCCCCCCCEEEEEEEEEECCCCEEEEEEECCCCCCEECCCHHHHHHHCCCCCCCE
ILVPQKAITRNTKGQAVAMVIEDGKVAARIVTTAEVIDNQWRITSGLKVGEQLIVEGLQK
EEECCHHHHCCCCCCEEEEEEECCCEEEEEEEEHHHHCCCEEEECCCHHHHHHHHHHHHH
IRPGAPVKAGPLAADKKPETQQK
HCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]