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The map label for this gene is narI [H]

Identifier: 167622121

GI number: 167622121

Start: 213128

End: 213805

Strand: Direct

Name: narI [H]

Synonym: Shal_0180

Alternate gene names: 167622121

Gene position: 213128-213805 (Clockwise)

Preceding gene: 167622120

Following gene: 167622122

Centisome position: 4.08

GC content: 43.36

Gene sequence:

>678_bases
ATGTCAAATTTAAATTTTTTATTGTTTGGGATTTATCCCTTTATCGCTATAACGGTATTTGTTGTAGGTTGTTGGATCCG
CTACGACCGTGAGCCCTATTCATGGAAGGCTGACTCGAGTCAGATATTCGATCAAAAGGGCTTCCGTTTGGCCAGCTATC
TGTTCCATATCGGTGTCATCTTTATCTTAGGTGGTCACTTCGTTGGTTTGCTAACACCGGAATCTATCTATCACCACTTC
ATTTCTACGCCTGACAAGCAGTTACTGGCTATGGTGAGTGGTGGCTTCTTTGGCATACTGTGCTTTATTGGTACTGTGAT
GTTGATTCGTCGTCGTTTAAACAATGATAGAGTGAAGGCGAATACCAAGCGTTCGGATCTAGTGGTGTTGTTCTTGCTGC
TACTACAGGTCTCTTTAGGCTTGTTGACGATTATCGCATCGGCTCAGCACTTAGATGGCTCGGTGATGGTATTACTCGCT
AATTGGGCGCAAAGTATTGTGACTCTGCAGGGCGTTCAAGCGACAGAGTCAATTGAGTCGGTCAATATCATCTATAAGTT
ACACATCCTTGTAGGATTAACCTTGATAATGATATTCCCATTCACCCGTTTGGTGCATGTGATTAGTGTTCCGGTGAAGT
ACTTCACCAGAAGTTATCAGATCGTTCGTCAGCGTTAA

Upstream 100 bases:

>100_bases
CAATCGCAGAACTCGCTTGATCCCAATACTGTAAATCAAGTGCCTATTAAGTGGCATGACGCAGATGCTGCACAAGTTGC
AGGATTGTAAGGAGAAAATA

Downstream 100 bases:

>100_bases
TTTACTCATCGGGCTCAGTTTTAGGTGGTGTTACTACTTATTATTGAGCCCTTTTTTTAGTAATTAACACATTTTTATTT
AGCGGGATTGAGAAGAGCAG

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSNLNFLLFGIYPFIAITVFVVGCWIRYDREPYSWKADSSQIFDQKGFRLASYLFHIGVIFILGGHFVGLLTPESIYHHF
ISTPDKQLLAMVSGGFFGILCFIGTVMLIRRRLNNDRVKANTKRSDLVVLFLLLLQVSLGLLTIIASAQHLDGSVMVLLA
NWAQSIVTLQGVQATESIESVNIIYKLHILVGLTLIMIFPFTRLVHVISVPVKYFTRSYQIVRQR

Sequences:

>Translated_225_residues
MSNLNFLLFGIYPFIAITVFVVGCWIRYDREPYSWKADSSQIFDQKGFRLASYLFHIGVIFILGGHFVGLLTPESIYHHF
ISTPDKQLLAMVSGGFFGILCFIGTVMLIRRRLNNDRVKANTKRSDLVVLFLLLLQVSLGLLTIIASAQHLDGSVMVLLA
NWAQSIVTLQGVQATESIESVNIIYKLHILVGLTLIMIFPFTRLVHVISVPVKYFTRSYQIVRQR
>Mature_224_residues
SNLNFLLFGIYPFIAITVFVVGCWIRYDREPYSWKADSSQIFDQKGFRLASYLFHIGVIFILGGHFVGLLTPESIYHHFI
STPDKQLLAMVSGGFFGILCFIGTVMLIRRRLNNDRVKANTKRSDLVVLFLLLLQVSLGLLTIIASAQHLDGSVMVLLAN
WAQSIVTLQGVQATESIESVNIIYKLHILVGLTLIMIFPFTRLVHVISVPVKYFTRSYQIVRQR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=225, Percent_Identity=51.1111111111111, Blast_Score=242, Evalue=1e-65,
Organism=Escherichia coli, GI1787738, Length=222, Percent_Identity=49.0990990990991, Blast_Score=223, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25527; Mature: 25396

Theoretical pI: Translated: 10.08; Mature: 10.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLNFLLFGIYPFIAITVFVVGCWIRYDREPYSWKADSSQIFDQKGFRLASYLFHIGVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHH
FILGGHFVGLLTPESIYHHFISTPDKQLLAMVSGGFFGILCFIGTVMLIRRRLNNDRVKA
HHHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEC
NTKRSDLVVLFLLLLQVSLGLLTIIASAQHLDGSVMVLLANWAQSIVTLQGVQATESIES
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VNIIYKLHILVGLTLIMIFPFTRLVHVISVPVKYFTRSYQIVRQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLNFLLFGIYPFIAITVFVVGCWIRYDREPYSWKADSSQIFDQKGFRLASYLFHIGVI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHH
FILGGHFVGLLTPESIYHHFISTPDKQLLAMVSGGFFGILCFIGTVMLIRRRLNNDRVKA
HHHHHHHHEECCHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEC
NTKRSDLVVLFLLLLQVSLGLLTIIASAQHLDGSVMVLLANWAQSIVTLQGVQATESIES
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VNIIYKLHILVGLTLIMIFPFTRLVHVISVPVKYFTRSYQIVRQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]