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The map label for this gene is narK [H]

Identifier: 167622114

GI number: 167622114

Start: 201090

End: 202364

Strand: Direct

Name: narK [H]

Synonym: Shal_0173

Alternate gene names: 167622114

Gene position: 201090-202364 (Clockwise)

Preceding gene: 167622112

Following gene: 167622117

Centisome position: 3.85

GC content: 48.16

Gene sequence:

>1275_bases
ATGAGCCACCAGGAACGTAAATCATATCTCGCCCTTACTCTAGCGACACTGGCATTTGCTGCATGTTTTTCTGTTTGGAC
TCTATATTCAATTCTGGGGATTGAGCTACAACGTCAGCTCAATCTGAGTTCGACTGAGTTTGGTCTGTTACTGGCTGCGC
CCATTTTTACCGGGGCGATCTTTCGTATACCGATAGGTTTTTTAGCTGAGAAAGTCTCTTGTCGGTCACTGTTTTTCTGG
CAAATGCTTTCGGTTGTTCCTCCACTTTTTTACCTTCCTTATGTCGAAACATTTTCCGGTTATATCACCTTGGGGTTCTT
AATCGGGATTAGTGGTGTCTCCTTCACCATCGGCATCCGCTACGTTACCGATTGGTTTGAAACCAAGCACCAAGGTTTTG
CGCTAGGGGTGTTCGGCGCCGGTAACGCCGGGGCGGCGATCACTCTGGTGCTGGTACCTCTGGTCGTGGAGTTTTATGGC
TGGAAAACCATCGGCACAGTGTACGGATTTGGCATGATATTTATGGCTATCCTGTTTCGCCTATTTGCTCCAGAGGTTAA
TGCCTTTTATAAGAATAAGCAGCAAACTAAGAGTTTTGAGTTCTATTGGGAGCCATTAAAGCAGCTGCAGGTATGGCGCT
TTGGGCTCTATTACTATTTTGTTTTTGGCAGCTTTCTGGCCTTGCTGCTTTGGCTGCCACAATATTATGTATCGGCATAC
GGTCTGTCTTTGACCCAATCCTTGGCGCTGACTCTGCTGTTTGTTACTAGCTCTAGTATTGTGCGTGCGGTGGGGGGCTG
GTTTGCCGACACCTTTGGTGCTCGCACGGTTAACTGGAGCGCCTTTTGGATCTGCATGGTGTGTCTGTTTTTCCTGAGTT
ACCCTCCAACAACCATGACGGTCCACGGCGTAGAAAAAGATGTCACCGTTGCGTTTGAAGTTAATGTTTGGCTGTTTACT
CTGCTGATTGTGATCATCGGTTTGGCACAGGGTTTTGGTCGAGCCAGTATCTACAAGACAATATATGACTATTATCCACA
CCATATGGGGAGCGTCGGGGGCGTCGTCGCTGCAGTGGGGGCGCTGGGTGGCTGTACGCTACCGATTTTATTTGGTCTGT
CTGTTGACGTGATCGGCGTATATAGCGCGTGCTTTATGCTGCTGTATGGCGTGTTAGCTCTGTGTATGGTGGTGATGTAC
TTTGCTATTAGGGCCGAGCGGGAGCAGAAACTGCTTAAGGAAGCGATTGCGAACAATTTCTTGGAAAAGGATTAG

Upstream 100 bases:

>100_bases
AGTAGTTTTTGATTTTTATTTAAGCATTGGTTATTGTTGATGGGTTAAGTTGTCGTTTTAGTGTAATTCTCTTATATCAT
TGATGATTAAGGTTTTAATT

Downstream 100 bases:

>100_bases
CCCTAACGTACTGCACGCTAAGGCTCATTGATGTCGTCGCTCTATATCGGAGAGTGCTTCTCTACTAGAGGAGTAAGCTA
GTAGAGAGCGACGACAAACT

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MSHQERKSYLALTLATLAFAACFSVWTLYSILGIELQRQLNLSSTEFGLLLAAPIFTGAIFRIPIGFLAEKVSCRSLFFW
QMLSVVPPLFYLPYVETFSGYITLGFLIGISGVSFTIGIRYVTDWFETKHQGFALGVFGAGNAGAAITLVLVPLVVEFYG
WKTIGTVYGFGMIFMAILFRLFAPEVNAFYKNKQQTKSFEFYWEPLKQLQVWRFGLYYYFVFGSFLALLLWLPQYYVSAY
GLSLTQSLALTLLFVTSSSIVRAVGGWFADTFGARTVNWSAFWICMVCLFFLSYPPTTMTVHGVEKDVTVAFEVNVWLFT
LLIVIIGLAQGFGRASIYKTIYDYYPHHMGSVGGVVAAVGALGGCTLPILFGLSVDVIGVYSACFMLLYGVLALCMVVMY
FAIRAEREQKLLKEAIANNFLEKD

Sequences:

>Translated_424_residues
MSHQERKSYLALTLATLAFAACFSVWTLYSILGIELQRQLNLSSTEFGLLLAAPIFTGAIFRIPIGFLAEKVSCRSLFFW
QMLSVVPPLFYLPYVETFSGYITLGFLIGISGVSFTIGIRYVTDWFETKHQGFALGVFGAGNAGAAITLVLVPLVVEFYG
WKTIGTVYGFGMIFMAILFRLFAPEVNAFYKNKQQTKSFEFYWEPLKQLQVWRFGLYYYFVFGSFLALLLWLPQYYVSAY
GLSLTQSLALTLLFVTSSSIVRAVGGWFADTFGARTVNWSAFWICMVCLFFLSYPPTTMTVHGVEKDVTVAFEVNVWLFT
LLIVIIGLAQGFGRASIYKTIYDYYPHHMGSVGGVVAAVGALGGCTLPILFGLSVDVIGVYSACFMLLYGVLALCMVVMY
FAIRAEREQKLLKEAIANNFLEKD
>Mature_423_residues
SHQERKSYLALTLATLAFAACFSVWTLYSILGIELQRQLNLSSTEFGLLLAAPIFTGAIFRIPIGFLAEKVSCRSLFFWQ
MLSVVPPLFYLPYVETFSGYITLGFLIGISGVSFTIGIRYVTDWFETKHQGFALGVFGAGNAGAAITLVLVPLVVEFYGW
KTIGTVYGFGMIFMAILFRLFAPEVNAFYKNKQQTKSFEFYWEPLKQLQVWRFGLYYYFVFGSFLALLLWLPQYYVSAYG
LSLTQSLALTLLFVTSSSIVRAVGGWFADTFGARTVNWSAFWICMVCLFFLSYPPTTMTVHGVEKDVTVAFEVNVWLFTL
LIVIIGLAQGFGRASIYKTIYDYYPHHMGSVGGVVAAVGALGGCTLPILFGLSVDVIGVYSACFMLLYGVLALCMVVMYF
AIRAEREQKLLKEAIANNFLEKD

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI2367379, Length=243, Percent_Identity=24.2798353909465, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1789152, Length=429, Percent_Identity=25.4079254079254, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47435; Mature: 47304

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure 
SHQERKSYLALTLATLAFAACFSVWTLYSILGIELQRQLNLSSTEFGLLLAAPIFTGAI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH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CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]