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The map label for this gene is narK [H]

Identifier: 167622117

GI number: 167622117

Start: 205341

End: 206945

Strand: Direct

Name: narK [H]

Synonym: Shal_0176

Alternate gene names: 167622117

Gene position: 205341-206945 (Clockwise)

Preceding gene: 167622114

Following gene: 167622118

Centisome position: 3.93

GC content: 45.92

Gene sequence:

>1605_bases
ATGTCCGATATAGATAAATGGAATGTCGAGGATCCCAAATTTTGGGACGGAGAAGGGAAAAAAATCGCCAATAGAAACCT
TTGGATATCAATCCCGAGTTTACTTTGCGGTTTTGCGGTTTGGCTTTACTGGGGAATTATCACTATCCAGATGTTGAATC
TGGGTTTCCCATATGCAACATCAGAACTGTTTACCTTGATGGCTATTGCCGGCTTAACCGGCGCAACATTGCGTATTCCA
AGCAGTTTTTTCATTCGTTTATGTGGTGGCAGAAATACCATCGTCTTTACCACCGCATTATTAATGATCCCGGCTATAGG
CACGGGTATTGCCTTGCAGGATAAGAATACACCGCTATGGGTATTCCAAGGTCTTGCTTTATTGTCAGGTTTAGGTGGCG
GTAACTTCGCCTCTTCAATGTCAAATATCAGTTTCTTCTTCCCTAAGAAGCAGCAGGGATTGGCACTTGGATTAAATGCG
GGTCTAGGTAACTTTGGTGTAACCACCATGCAGATCCTAGTTCCACTAGTGATGACATTTGGCATCTTCGGCGGTGAGCC
GATGGAACTGGTTAATACCTCTGGCACCTTGATTGGCAAGATCCCTGCGGGATCTGATGCTTGGATTCAAAATGCGGGTT
ATGTCTGGCTGCTGTTTTTGATTCCGTTAGCCGTGGTTGGCTGGTTTGGTATGAATAATATCAAGGCATCTCATGTCACA
CCGGGATTACCTAGTCCTGTAGGCTCTTTCGGCATTATTTCTGGCATGCTGGCTATCGGTTTCCTTACTTCTATCCTTGG
CCTGTGGTTGATGCTACCGGAAAAGGTCGGTGGTTCTGGGATAGAGTTGAGCAAGTGGATCGTACTGCCAATTGTGATCG
CTCTGACCGTGTTCTTGCTTAAGCTTATTCCTGGTCAGGTTAAGGAAAACTTAACCCACCAATACAAAATATTCGGCAAT
ACCCATACCTGGGTAATGAGTGTTATCTACACCATGACCTTTGGCTCTTTTATCGGTTTTTCTGCAGGCTTTGGTCTATC
AATCAAAGTTATCTTCGGATATCAGCATGTGATGGTCGATGGTGTGATGACCCACGACATTATTAACCCTAACGGCCCAA
GTGCACTGATGTACGCATGGATGGGGCCTTTCATCGGTGCGCTTATTCGCCCTGTCGGTGGTTGGATTGCTGATAAGTTT
GGTGGTGCAAAAGTGACACAGATCTGTGCCATTGTCATGGTGGCGAGCGCTCTTGGTGTGGCTTATTTCATGAAGGCTGC
ATATTCCTCGGAAACCCCACAAGAGTTCTTTGTTCCGTTCCTTGTGTTGTTCCTAGTGCTGTTTGCCGCAACGGGTATTG
GTAACGGTTCGACTTTCCGAACTATCTCTATGGTGTTTGATAAAGAACAGGCTGGACCGGTTCTTGGTTGGACGTCTGCC
GTTGCTGCTTATGGCGCATTCATCATTCCTAAAGTGCTTGGTGAGCAGATTAAGCTGACAACCCCTGAAAATGCATTGTA
TGGCTTCGCTGTTTTCTATGGCTTATGCATCTTAGTTAACTGGTGGTTCTATCTGCGTCCAGGTGCGGAATACAAGAATC
CATAA

Upstream 100 bases:

>100_bases
GATGTTTCAAATATACATAAAAAGAAACTGATTATCCTGCCCAATATCAGCAACTGCAACTGGCCAGTTTAATTAAGTGA
TTTAATGATTGGAGAAACCA

Downstream 100 bases:

>100_bases
TGACTTTGCCGTTTTCTACACGACGGCAATCCTAGCCAATAAACGCAAAATATAACAATGAGAAGTGGATGATATCGGCA
AGATATCATCCACTCAAATG

Product: major facilitator transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSDIDKWNVEDPKFWDGEGKKIANRNLWISIPSLLCGFAVWLYWGIITIQMLNLGFPYATSELFTLMAIAGLTGATLRIP
SSFFIRLCGGRNTIVFTTALLMIPAIGTGIALQDKNTPLWVFQGLALLSGLGGGNFASSMSNISFFFPKKQQGLALGLNA
GLGNFGVTTMQILVPLVMTFGIFGGEPMELVNTSGTLIGKIPAGSDAWIQNAGYVWLLFLIPLAVVGWFGMNNIKASHVT
PGLPSPVGSFGIISGMLAIGFLTSILGLWLMLPEKVGGSGIELSKWIVLPIVIALTVFLLKLIPGQVKENLTHQYKIFGN
THTWVMSVIYTMTFGSFIGFSAGFGLSIKVIFGYQHVMVDGVMTHDIINPNGPSALMYAWMGPFIGALIRPVGGWIADKF
GGAKVTQICAIVMVASALGVAYFMKAAYSSETPQEFFVPFLVLFLVLFAATGIGNGSTFRTISMVFDKEQAGPVLGWTSA
VAAYGAFIIPKVLGEQIKLTTPENALYGFAVFYGLCILVNWWFYLRPGAEYKNP

Sequences:

>Translated_534_residues
MSDIDKWNVEDPKFWDGEGKKIANRNLWISIPSLLCGFAVWLYWGIITIQMLNLGFPYATSELFTLMAIAGLTGATLRIP
SSFFIRLCGGRNTIVFTTALLMIPAIGTGIALQDKNTPLWVFQGLALLSGLGGGNFASSMSNISFFFPKKQQGLALGLNA
GLGNFGVTTMQILVPLVMTFGIFGGEPMELVNTSGTLIGKIPAGSDAWIQNAGYVWLLFLIPLAVVGWFGMNNIKASHVT
PGLPSPVGSFGIISGMLAIGFLTSILGLWLMLPEKVGGSGIELSKWIVLPIVIALTVFLLKLIPGQVKENLTHQYKIFGN
THTWVMSVIYTMTFGSFIGFSAGFGLSIKVIFGYQHVMVDGVMTHDIINPNGPSALMYAWMGPFIGALIRPVGGWIADKF
GGAKVTQICAIVMVASALGVAYFMKAAYSSETPQEFFVPFLVLFLVLFAATGIGNGSTFRTISMVFDKEQAGPVLGWTSA
VAAYGAFIIPKVLGEQIKLTTPENALYGFAVFYGLCILVNWWFYLRPGAEYKNP
>Mature_533_residues
SDIDKWNVEDPKFWDGEGKKIANRNLWISIPSLLCGFAVWLYWGIITIQMLNLGFPYATSELFTLMAIAGLTGATLRIPS
SFFIRLCGGRNTIVFTTALLMIPAIGTGIALQDKNTPLWVFQGLALLSGLGGGNFASSMSNISFFFPKKQQGLALGLNAG
LGNFGVTTMQILVPLVMTFGIFGGEPMELVNTSGTLIGKIPAGSDAWIQNAGYVWLLFLIPLAVVGWFGMNNIKASHVTP
GLPSPVGSFGIISGMLAIGFLTSILGLWLMLPEKVGGSGIELSKWIVLPIVIALTVFLLKLIPGQVKENLTHQYKIFGNT
HTWVMSVIYTMTFGSFIGFSAGFGLSIKVIFGYQHVMVDGVMTHDIINPNGPSALMYAWMGPFIGALIRPVGGWIADKFG
GAKVTQICAIVMVASALGVAYFMKAAYSSETPQEFFVPFLVLFLVLFAATGIGNGSTFRTISMVFDKEQAGPVLGWTSAV
AAYGAFIIPKVLGEQIKLTTPENALYGFAVFYGLCILVNWWFYLRPGAEYKNP

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=244, Percent_Identity=43.8524590163934, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1787743, Length=244, Percent_Identity=43.0327868852459, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57703; Mature: 57572

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDIDKWNVEDPKFWDGEGKKIANRNLWISIPSLLCGFAVWLYWGIITIQMLNLGFPYAT
CCCCCCCCCCCCCEECCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SELFTLMAIAGLTGATLRIPSSFFIRLCGGRNTIVFTTALLMIPAIGTGIALQDKNTPLW
HHHHHHHHHHCCCCCEEECCHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHH
VFQGLALLSGLGGGNFASSMSNISFFFPKKQQGLALGLNAGLGNFGVTTMQILVPLVMTF
HHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
GIFGGEPMELVNTSGTLIGKIPAGSDAWIQNAGYVWLLFLIPLAVVGWFGMNNIKASHVT
HCCCCCCHHHCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PGLPSPVGSFGIISGMLAIGFLTSILGLWLMLPEKVGGSGIELSKWIVLPIVIALTVFLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KLIPGQVKENLTHQYKIFGNTHTWVMSVIYTMTFGSFIGFSAGFGLSIKVIFGYQHVMVD
HHCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHC
GVMTHDIINPNGPSALMYAWMGPFIGALIRPVGGWIADKFGGAKVTQICAIVMVASALGV
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AYFMKAAYSSETPQEFFVPFLVLFLVLFAATGIGNGSTFRTISMVFDKEQAGPVLGWTSA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCHHHH
VAAYGAFIIPKVLGEQIKLTTPENALYGFAVFYGLCILVNWWFYLRPGAEYKNP
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDIDKWNVEDPKFWDGEGKKIANRNLWISIPSLLCGFAVWLYWGIITIQMLNLGFPYAT
CCCCCCCCCCCCEECCCCCEEECCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
SELFTLMAIAGLTGATLRIPSSFFIRLCGGRNTIVFTTALLMIPAIGTGIALQDKNTPLW
HHHHHHHHHHCCCCCEEECCHHHHHEECCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHH
VFQGLALLSGLGGGNFASSMSNISFFFPKKQQGLALGLNAGLGNFGVTTMQILVPLVMTF
HHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
GIFGGEPMELVNTSGTLIGKIPAGSDAWIQNAGYVWLLFLIPLAVVGWFGMNNIKASHVT
HCCCCCCHHHCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PGLPSPVGSFGIISGMLAIGFLTSILGLWLMLPEKVGGSGIELSKWIVLPIVIALTVFLL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
KLIPGQVKENLTHQYKIFGNTHTWVMSVIYTMTFGSFIGFSAGFGLSIKVIFGYQHVMVD
HHCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHC
GVMTHDIINPNGPSALMYAWMGPFIGALIRPVGGWIADKFGGAKVTQICAIVMVASALGV
CHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AYFMKAAYSSETPQEFFVPFLVLFLVLFAATGIGNGSTFRTISMVFDKEQAGPVLGWTSA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECHHCCCCCCCHHHH
VAAYGAFIIPKVLGEQIKLTTPENALYGFAVFYGLCILVNWWFYLRPGAEYKNP
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]