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The map label for this gene is sigE [H]

Identifier: 167040624

GI number: 167040624

Start: 2009793

End: 2010518

Strand: Reverse

Name: sigE [H]

Synonym: Teth514_2001

Alternate gene names: 167040624

Gene position: 2010518-2009793 (Counterclockwise)

Preceding gene: 167040625

Following gene: 167040623

Centisome position: 81.82

GC content: 28.37

Gene sequence:

>726_bases
GTGCAACTAAAAACAAAAGTAAAAATAGAAATGCTATTGTTGTGGAAAAAAATCCTTGATTTTTTAGATTTATCTGAAGG
CATATTTTATATTGGAGGAAGTGAAGCATTACCTCCACCTCTTTCTGTAGAAGAAGAAATGTATTTAATTTCAAAGATGG
AAAAAGGAGATAATGAAGCAAAAACGGTACTTATTGAAAGAAATTTAAGATTGGTAGTATATATAGCAAAAAAATTTGAA
AACACAGGAGTAGGAATCGAAGATTTAATATCTATTGGCACGATTGGACTTATAAAAGCTATTAATACTTTCAATCCTAA
GAAAAAAATAAAATTAGCTACTTATGCTTCTCGATGCATTGAGAATGAGATATTAATGTATTTAAGAAGGAATAGCAAAA
CTAAATTAGAGGTTTCTTTTGATGAACCTTTAAATGTAGATTGGGATGGAAATGAACTTTTGCTTTCAGATATTTTAGGC
ACTGATAATGAAACAGTTTATAAATATATCGAAGATGAAGTAGAAAAGGAATTATTGACTTCTGCATTAAAGAAATTACC
AGATAGAGAAAAAAAGATAATAGGACTTAGATTTGGATTAGAGGGTGGAGTAGAAAAGACTCAAAAAGAAGTGGCAGATA
TGTTGGGAATTTCACAGTCCTATATTTCTCGCTTAGAAAAAAAGATTTTAAAAAGATTAAAAAAAGAAATGAATAGACTA
GTTTAA

Upstream 100 bases:

>100_bases
GGAATGTAATAGTAGGAGTATACAAAAGCCAAATAGATAAATATGGTAATTATGCGGCGTTGTTAGCACCAGAAATTTTA
GTATAATGGGGGTGTTGGAA

Downstream 100 bases:

>100_bases
GCGTTTCTGTATATTTTTTTATCCTGCCGGCAATACTGTTAATAAGCTAAACATGTGGGGGCAGGATTTATGAATAACAA
AGTTGAAATTTGCGGTGTCA

Product: sporulation sigma factor SigE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MQLKTKVKIEMLLLWKKILDFLDLSEGIFYIGGSEALPPPLSVEEEMYLISKMEKGDNEAKTVLIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPKKKIKLATYASRCIENEILMYLRRNSKTKLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVEKELLTSALKKLPDREKKIIGLRFGLEGGVEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V

Sequences:

>Translated_241_residues
MQLKTKVKIEMLLLWKKILDFLDLSEGIFYIGGSEALPPPLSVEEEMYLISKMEKGDNEAKTVLIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPKKKIKLATYASRCIENEILMYLRRNSKTKLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVEKELLTSALKKLPDREKKIIGLRFGLEGGVEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V
>Mature_241_residues
MQLKTKVKIEMLLLWKKILDFLDLSEGIFYIGGSEALPPPLSVEEEMYLISKMEKGDNEAKTVLIERNLRLVVYIAKKFE
NTGVGIEDLISIGTIGLIKAINTFNPKKKIKLATYASRCIENEILMYLRRNSKTKLEVSFDEPLNVDWDGNELLLSDILG
TDNETVYKYIEDEVEKELLTSALKKLPDREKKIIGLRFGLEGGVEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
V

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=250, Percent_Identity=27.6, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1789871, Length=105, Percent_Identity=40.9523809523809, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=27.8761061946903, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014200
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27618; Mature: 27618

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKTKVKIEMLLLWKKILDFLDLSEGIFYIGGSEALPPPLSVEEEMYLISKMEKGDNEA
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHH
KTVLIERNLRLVVYIAKKFENTGVGIEDLISIGTIGLIKAINTFNPKKKIKLATYASRCI
HEEEEECCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ENEILMYLRRNSKTKLEVSFDEPLNVDWDGNELLLSDILGTDNETVYKYIEDEVEKELLT
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALKKLPDREKKIIGLRFGLEGGVEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
HHHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
V
C
>Mature Secondary Structure
MQLKTKVKIEMLLLWKKILDFLDLSEGIFYIGGSEALPPPLSVEEEMYLISKMEKGDNEA
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHH
KTVLIERNLRLVVYIAKKFENTGVGIEDLISIGTIGLIKAINTFNPKKKIKLATYASRCI
HEEEEECCCEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
ENEILMYLRRNSKTKLEVSFDEPLNVDWDGNELLLSDILGTDNETVYKYIEDEVEKELLT
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SALKKLPDREKKIIGLRFGLEGGVEKTQKEVADMLGISQSYISRLEKKILKRLKKEMNRL
HHHHHCCCCHHEEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHC
V
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]