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The map label for this gene is folD [H]

Identifier: 167040173

GI number: 167040173

Start: 1557946

End: 1558806

Strand: Direct

Name: folD [H]

Synonym: Teth514_1535

Alternate gene names: 167040173

Gene position: 1557946-1558806 (Clockwise)

Preceding gene: 167040172

Following gene: 167040174

Centisome position: 63.4

GC content: 35.31

Gene sequence:

>861_bases
ATGAAGGATTGGAGGAAGGTGAATCAAATGATAATTGATGGTAAGGAGATTTCTAAAAAGATAAGAGAAGAGGTAAAAAG
AGAGATAAAGAAGTTTGGCTACAAGCCCAAATTAGCGATATTGATGGCGGGAGATGATGAGTCTTCAAAGGTTTACGCAA
ACTCTAAAGTTAAAGCCTGTGAAAATGTGGGGATTGAAGCTAAAATCTATTATTTTTCAGAAAGTGAAGAGTCAAAATTT
TTTGACACCTTAAAGTTTTTAAATGAAGATAAAAATACTCACGGCATAATGATAGAAATGCCCCTTCCTAAAAACTTTAA
TGCTGAGTTGGTCTACGATACTATAAACCCTTATAAAGACGTTGATTGCATATCTAATTATAATATGGGAAGGCTATTTG
CAGGGAAACCACTTTTTGTACCTTGTACTCCTAAAGCAATTTTACATATACTGGAAGGTATTAATACTCAATTTGAGGGA
AAACATGCTGTTGTAATAGGAAGAAGTAATATCTTAGGAAAACCTGTAGCTAAGCTTTTATTAGACAAAAATTGTACGGT
TACTGTGTGCCATTCAAAAACAAAAGACTTGGCTTATTATACTAGACAAGCGGATATTTTAGTGTTAGCGGCTGGTAAGA
TGAATTTGGTCAAGGGAGATATGGTTAAGGAGGGAGCAATACTTATAGATGCGGGTATAAACGTTTACCAAGATAATATA
TATGGAGATGCAGACTTTGAAAGTGTAAAAGACCTGTGTTCTTACGTAACACCAGTTCCAGGAGGAGTTGGACCTGTGAC
GACAGCTATGATACTTAAAAATACCCTGGAGGCTTTTAAATATGCAGTTAAAGGCGCTTGA

Upstream 100 bases:

>100_bases
GTAAATATTTCTATAAACGAAGCAGTAGAAATAGCCAAAAAATACAGCACAGAAGATTCTCCTGCTTTTATAAACGGTTT
GTTGGGAGCATTTGTAAGGG

Downstream 100 bases:

>100_bases
AGTCAGAGAAATTACAGACTATATCAAAAAAATGATGGATAATGATATAATTTTGAGAAATGTGCGGGTTAAAGGAGAAA
TTTCTAATTTGAAATACCAT

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKDWRKVNQMIIDGKEISKKIREEVKREIKKFGYKPKLAILMAGDDESSKVYANSKVKACENVGIEAKIYYFSESEESKF
FDTLKFLNEDKNTHGIMIEMPLPKNFNAELVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAILHILEGINTQFEG
KHAVVIGRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYYTRQADILVLAAGKMNLVKGDMVKEGAILIDAGINVYQDNI
YGDADFESVKDLCSYVTPVPGGVGPVTTAMILKNTLEAFKYAVKGA

Sequences:

>Translated_286_residues
MKDWRKVNQMIIDGKEISKKIREEVKREIKKFGYKPKLAILMAGDDESSKVYANSKVKACENVGIEAKIYYFSESEESKF
FDTLKFLNEDKNTHGIMIEMPLPKNFNAELVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAILHILEGINTQFEG
KHAVVIGRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYYTRQADILVLAAGKMNLVKGDMVKEGAILIDAGINVYQDNI
YGDADFESVKDLCSYVTPVPGGVGPVTTAMILKNTLEAFKYAVKGA
>Mature_286_residues
MKDWRKVNQMIIDGKEISKKIREEVKREIKKFGYKPKLAILMAGDDESSKVYANSKVKACENVGIEAKIYYFSESEESKF
FDTLKFLNEDKNTHGIMIEMPLPKNFNAELVYDTINPYKDVDCISNYNMGRLFAGKPLFVPCTPKAILHILEGINTQFEG
KHAVVIGRSNILGKPVAKLLLDKNCTVTVCHSKTKDLAYYTRQADILVLAAGKMNLVKGDMVKEGAILIDAGINVYQDNI
YGDADFESVKDLCSYVTPVPGGVGPVTTAMILKNTLEAFKYAVKGA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=287, Percent_Identity=41.4634146341463, Blast_Score=224, Evalue=5e-59,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=37.6712328767123, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=34.1216216216216, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI36796743, Length=227, Percent_Identity=26.8722466960352, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=42.3357664233577, Blast_Score=226, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17568735, Length=287, Percent_Identity=39.7212543554007, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=40, Blast_Score=220, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321643, Length=295, Percent_Identity=39.3220338983051, Blast_Score=207, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6322933, Length=294, Percent_Identity=24.4897959183673, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=40.8450704225352, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=40.8450704225352, Blast_Score=213, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=40.8450704225352, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=40.8450704225352, Blast_Score=213, Evalue=2e-55,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=37.4570446735395, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=37.4570446735395, Blast_Score=196, Evalue=1e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31869; Mature: 31869

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDWRKVNQMIIDGKEISKKIREEVKREIKKFGYKPKLAILMAGDDESSKVYANSKVKAC
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHH
ENVGIEAKIYYFSESEESKFFDTLKFLNEDKNTHGIMIEMPLPKNFNAELVYDTINPYKD
HCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCC
VDCISNYNMGRLFAGKPLFVPCTPKAILHILEGINTQFEGKHAVVIGRSNILGKPVAKLL
CHHHCCCCCCEEECCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHH
LDKNCTVTVCHSKTKDLAYYTRQADILVLAAGKMNLVKGDMVKEGAILIDAGINVYQDNI
HCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCEEEECCCCCCCCEEEECCCEEEECCC
YGDADFESVKDLCSYVTPVPGGVGPVTTAMILKNTLEAFKYAVKGA
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKDWRKVNQMIIDGKEISKKIREEVKREIKKFGYKPKLAILMAGDDESSKVYANSKVKAC
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHH
ENVGIEAKIYYFSESEESKFFDTLKFLNEDKNTHGIMIEMPLPKNFNAELVYDTINPYKD
HCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCC
VDCISNYNMGRLFAGKPLFVPCTPKAILHILEGINTQFEGKHAVVIGRSNILGKPVAKLL
CHHHCCCCCCEEECCCEEEEECCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHH
LDKNCTVTVCHSKTKDLAYYTRQADILVLAAGKMNLVKGDMVKEGAILIDAGINVYQDNI
HCCCCEEEEECCCCCCCEEEECCCCEEEEECCCCEEEECCCCCCCCEEEECCCEEEECCC
YGDADFESVKDLCSYVTPVPGGVGPVTTAMILKNTLEAFKYAVKGA
CCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]