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The map label for this gene is proB

Identifier: 167040157

GI number: 167040157

Start: 1546854

End: 1547972

Strand: Direct

Name: proB

Synonym: Teth514_1519

Alternate gene names: 167040157

Gene position: 1546854-1547972 (Clockwise)

Preceding gene: 167040156

Following gene: 167040158

Centisome position: 62.95

GC content: 33.78

Gene sequence:

>1119_bases
ATGAGAATTGTAGTAAAAGTTGGTACAAGCACTTTGACATATGAAAATGGTAAATTGAATTTAGAAATAATGGAGAAGTT
AGTAAGGCAAATTGCTAATTTATTAAACAGAGGAGAGGAAGTAGTATTAGTAACTTCTGGGGCAATTGGGGCGGGAATGG
GAAAATTAAATTTAAAGGAAAAACCTAAAACCATCCCAGAAAAGCAATCCTTAGCGGCTATAGGGCAAGGGCTTTTAATT
GAAATTTATGAAAAGTTTTTTAATGAATATGGCAAAATTACTGCGCAAGTTCTTTTGACTAAGGAAGATTTTAGCGATAG
AAGAAGATATTTAAATGTCAGTTATACCTTGTCAAACCTGCTCAAATGGGGTGTTGTGCCAATAATAAATGAAAATGATA
CAGTAACTGTTGATGAAATTAAAATTGGAGACAATGACACTTTAGCGGCGCTGCTTGCTAGTTTAGTTGAGGCTGATATT
TTAATAATTTTAACTGACATTGATGGGCTTTATGATAAAGACCCTCGAATTTATAAAGAGGCAAAAATTATAGAAGTCGT
AGAAGAGTTTTCAGATGAATTGTTTAAAATTGCTGGTAGTGCTGGGACAAAAAGAGGAACTGGGGGAATGTATACAAAGA
TTCAGGCAGCAAAGATATGTTGGAATTCAGGAGTTAAGATGATTATAGCGAATGGGAAAATTGACAATGTATTAAATCAA
ATAGCAAATGGCGAAAAAATTGGCACTACATTTTTGCCTATGAAAAAGCCAATAAGTAGCAGAAAAGTTTGGATTGCTTT
TAATGCGAAAGTGAGCGGAAGACTTTTTATTGATGAAGGAGCAGCGAAAGCTATTATAAAGCATGGGAAAAGTCTATTAC
CAAGTGGAGTTGTAAAAACTGAAGGGGATTATGATGTAGGAGACTGTGTTGCAGTGGTTGACCATCAAGAAAAAGAGATT
GCCAGAGGACTTATTAATTATTCTTCAGAAGAAGTTGAAAAGATAAAAGGTTGTAAAACTCATGAGATAGAAAAGATATT
AGGTTATAAATATTACGATGAAGTAATCCATAGGGATAATCTTGTAATACTTGAAAGAGGTGAGAAGTTTGGAAGTTGA

Upstream 100 bases:

>100_bases
TGGAGCCTATTGTCATATGTGAATCGAGAGGAACTATGGCAGAAGATGCTTTAAAACTAAAAGAGATATATCTAAATATT
GCAAAAAGGATGTCGAAAGT

Downstream 100 bases:

>100_bases
AGTAAAGGCAAAACAGGCAAAAGCTGCGGCCAGAAGAATGGCTGTGTTAGATGAAAATACAAAGAATTTAGCTTTGAATC
ACATGGCAGATGCTTTAATA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRIVVKVGTSTLTYENGKLNLEIMEKLVRQIANLLNRGEEVVLVTSGAIGAGMGKLNLKEKPKTIPEKQSLAAIGQGLLI
EIYEKFFNEYGKITAQVLLTKEDFSDRRRYLNVSYTLSNLLKWGVVPIINENDTVTVDEIKIGDNDTLAALLASLVEADI
LIILTDIDGLYDKDPRIYKEAKIIEVVEEFSDELFKIAGSAGTKRGTGGMYTKIQAAKICWNSGVKMIIANGKIDNVLNQ
IANGEKIGTTFLPMKKPISSRKVWIAFNAKVSGRLFIDEGAAKAIIKHGKSLLPSGVVKTEGDYDVGDCVAVVDHQEKEI
ARGLINYSSEEVEKIKGCKTHEIEKILGYKYYDEVIHRDNLVILERGEKFGS

Sequences:

>Translated_372_residues
MRIVVKVGTSTLTYENGKLNLEIMEKLVRQIANLLNRGEEVVLVTSGAIGAGMGKLNLKEKPKTIPEKQSLAAIGQGLLI
EIYEKFFNEYGKITAQVLLTKEDFSDRRRYLNVSYTLSNLLKWGVVPIINENDTVTVDEIKIGDNDTLAALLASLVEADI
LIILTDIDGLYDKDPRIYKEAKIIEVVEEFSDELFKIAGSAGTKRGTGGMYTKIQAAKICWNSGVKMIIANGKIDNVLNQ
IANGEKIGTTFLPMKKPISSRKVWIAFNAKVSGRLFIDEGAAKAIIKHGKSLLPSGVVKTEGDYDVGDCVAVVDHQEKEI
ARGLINYSSEEVEKIKGCKTHEIEKILGYKYYDEVIHRDNLVILERGEKFGS
>Mature_372_residues
MRIVVKVGTSTLTYENGKLNLEIMEKLVRQIANLLNRGEEVVLVTSGAIGAGMGKLNLKEKPKTIPEKQSLAAIGQGLLI
EIYEKFFNEYGKITAQVLLTKEDFSDRRRYLNVSYTLSNLLKWGVVPIINENDTVTVDEIKIGDNDTLAALLASLVEADI
LIILTDIDGLYDKDPRIYKEAKIIEVVEEFSDELFKIAGSAGTKRGTGGMYTKIQAAKICWNSGVKMIIANGKIDNVLNQ
IANGEKIGTTFLPMKKPISSRKVWIAFNAKVSGRLFIDEGAAKAIIKHGKSLLPSGVVKTEGDYDVGDCVAVVDHQEKEI
ARGLINYSSEEVEKIKGCKTHEIEKILGYKYYDEVIHRDNLVILERGEKFGS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=289, Percent_Identity=33.9100346020761, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI62912457, Length=287, Percent_Identity=33.7979094076655, Blast_Score=146, Evalue=3e-35,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=43.6111111111111, Blast_Score=298, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI17569969, Length=314, Percent_Identity=32.484076433121, Blast_Score=151, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17569967, Length=314, Percent_Identity=32.484076433121, Blast_Score=151, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=35.7314148681055, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6321822, Length=413, Percent_Identity=34.6246973365617, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI21357643, Length=307, Percent_Identity=35.8306188925081, Blast_Score=155, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_THEP3 (B0K9C4)

Other databases:

- EMBL:   CP000924
- RefSeq:   YP_001665073.1
- ProteinModelPortal:   B0K9C4
- SMR:   B0K9C4
- GeneID:   5875377
- GenomeReviews:   CP000924_GR
- KEGG:   tpd:Teth39_1082
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   TPSE340099:TETH39_1082-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 41186; Mature: 41186

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVVKVGTSTLTYENGKLNLEIMEKLVRQIANLLNRGEEVVLVTSGAIGAGMGKLNLKE
CEEEEEECCCEEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
KPKTIPEKQSLAAIGQGLLIEIYEKFFNEYGKITAQVLLTKEDFSDRRRYLNVSYTLSNL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECCCHHCHHHEEEEHHHHHHH
LKWGVVPIINENDTVTVDEIKIGDNDTLAALLASLVEADILIILTDIDGLYDKDPRIYKE
HHHCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
AKIIEVVEEFSDELFKIAGSAGTKRGTGGMYTKIQAAKICWNSGVKMIIANGKIDNVLNQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCHHHHHHH
IANGEKIGTTFLPMKKPISSRKVWIAFNAKVSGRLFIDEGAAKAIIKHGKSLLPSGVVKT
HCCCCCCCCEECCCCCCCCCCEEEEEEECEECCEEEEECCHHHHHHHCCHHHCCCCCEEC
EGDYDVGDCVAVVDHQEKEIARGLINYSSEEVEKIKGCKTHEIEKILGYKYYDEVIHRDN
CCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCC
LVILERGEKFGS
EEEEECCCCCCC
>Mature Secondary Structure
MRIVVKVGTSTLTYENGKLNLEIMEKLVRQIANLLNRGEEVVLVTSGAIGAGMGKLNLKE
CEEEEEECCCEEEEECCEEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
KPKTIPEKQSLAAIGQGLLIEIYEKFFNEYGKITAQVLLTKEDFSDRRRYLNVSYTLSNL
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECCCHHCHHHEEEEHHHHHHH
LKWGVVPIINENDTVTVDEIKIGDNDTLAALLASLVEADILIILTDIDGLYDKDPRIYKE
HHHCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
AKIIEVVEEFSDELFKIAGSAGTKRGTGGMYTKIQAAKICWNSGVKMIIANGKIDNVLNQ
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCHHHHHHH
IANGEKIGTTFLPMKKPISSRKVWIAFNAKVSGRLFIDEGAAKAIIKHGKSLLPSGVVKT
HCCCCCCCCEECCCCCCCCCCEEEEEEECEECCEEEEECCHHHHHHHCCHHHCCCCCEEC
EGDYDVGDCVAVVDHQEKEIARGLINYSSEEVEKIKGCKTHEIEKILGYKYYDEVIHRDN
CCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHCCCC
LVILERGEKFGS
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA