Click here to switch to the map view.

The map label for this gene is ppsA [C]

Identifier: 167038778

GI number: 167038778

Start: 101675

End: 103378

Strand: Direct

Name: ppsA [C]

Synonym: Teth514_0105

Alternate gene names: 167038778

Gene position: 101675-103378 (Clockwise)

Preceding gene: 167038777

Following gene: 167038779

Centisome position: 4.14

GC content: 32.04

Gene sequence:

>1704_bases
ATGTCTGAGACTTTATTATATAAAAATTACAATCCGCGCTTAAACAGAAAAAACGAAGATAGAATTTTTGGGGAAGGGTA
TATTGGAGGAAAAGCTTCAGGACTTTGCTTTGCAGAAGAGGTATTAGAAAAATATATTGATGTTTTTAAGCAAAGTGTAA
AGATACCTGAAAGTTTTTTTATAGCTACTGATTATTATCAAATGTTTTTAGATTATAATAATTTAAATGATATAACGGAA
GATACTCCTTATGAGGAAGTAGAAATGAGATTTAGAGAAGCTAAATTTCCTCCAGAATTTAAAAAAGTGCTGACAGAGAT
TTTAAATAAAATGTATTATCCTTTAGCTGTGAGGTCTTCTTCTTTATTAGAAGATAATGTAAAATATTCTTTTGCTGGCA
AGTATTATACCACTTTTATTCCTAACAAGGGGACAGATAGAGAAAGGCTTAAACAATTAGAAAAAGCTATTAAAGAGATT
TATGTAAGCGTATATGGCCCAGATGCCGTCGCATATAGGAGAAAACATGCTCCTGATCAAAAAGAATTAATGGGGGTAAT
TGTGCAACAACTGATAGGTTCACAAAAAGGTATGTATTTTTATCCAGAGGTTTCTGGAGTAGGCTTCTCTCGAAATTACA
GACGGTGGACTGACAGAATTAAAATAGAAGATGGGGTTGTGAGATTAGTTTTTGGTTTAGGGACAAAGTGTACAGGTAGA
GGATATGCGAGAATTTTTTCTCTAACTAATTTGCGTTTAAGGCCCGAAGGCAATAATCCAAAAGAAATAGCGAAAAATTC
ACAGGAAACTTTTGATGTGCTAAATTTAATTACAGGGGAAGTTGAGACCTATAACATAAATGAAGTACCGCAATTTTTAA
CATATCACGATAAGATAGGTGATATTGCTCAAATATACTCAAAAAGAGAGAACGCTATTTTTGATATCAATATACTGAAT
AACAGCAATGATGTTTTTAATAAATATATTTTTACTTTTGAAAACTTTCCGAGGAAACACAGAGGATTTTTTAACATAAT
CTCTAATTTGTTTAAATATCTTGAGGAAGAAATGGGAATGGCAGTTGACATTGAATTCACTTATGACTTAAAAAAAGGCG
AATTTTATCTTCTTCAAACACGACCATTGAGTTCTTATGAGAGTTTCAGAAAAGTCCACATTCCTAAAGATATTGAAAAA
AAATGTGTCTTGTTGAAAGGGGATAGAATGCTTACAAATGGCATCTTGAAAAACATAAAATATATAGTATACGTAGACCA
TAAAGTTTACAGCCAATATAGAGATAAACATGAAATTGCAAGAATAATAGGTAGACTCAATAAAAGTATAGGAGATAGAT
ACATTCTTGTGGGTCCGGGAAGATGGGGTTCCTCTAATCCCTATTTAGGGGTACCTGTTATATACAACGAAATATCTAAT
GCTGCTATGATTGTAGAGCTTGGAATAAAAAATGGCGATTTTATGCCAGAGCTTTCTTATGGGACTCACTTTTTTACTGA
TTTAGACATTGATAACATTTTATACATGCCGGTATTTGATGGTTATGAAAATAATATTTTCAATGAAGAATGGTTTAAAA
GGGGGACATATATCGACACGGCTGTCAAAATATTTGAAGGACGATTTGATGCTTATTTAGATGGAGACAAAATGATAGGG
TATGTCATTTCTCATGAGAAGTAA

Upstream 100 bases:

>100_bases
ACCTCTCACAAAGAAATTTTGTGGGAGGTTTTTTGTTGTTTACTAAAAAATCTCATGTTATAATTTTTGTAAAATTAAAA
TCTCAATAGGCGGTGGAAAA

Downstream 100 bases:

>100_bases
GTCGATTTATGTATAAAAAGATATAAAAATAACAAAAAATGAGACTTAAGTCCTATTTAAAGCGCCAAAATAATGTAATA
TAATTTAAACTGTAAGAATG

Product: pyruvate phosphate dikinase, PEP/pyruvate-binding

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: Phosphoenolpyruvate Synthase/Pyruvate Phosphate Dikinase; Pyruvate Water Dikinase; Response Regulator Receiver Protein

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSETLLYKNYNPRLNRKNEDRIFGEGYIGGKASGLCFAEEVLEKYIDVFKQSVKIPESFFIATDYYQMFLDYNNLNDITE
DTPYEEVEMRFREAKFPPEFKKVLTEILNKMYYPLAVRSSSLLEDNVKYSFAGKYYTTFIPNKGTDRERLKQLEKAIKEI
YVSVYGPDAVAYRRKHAPDQKELMGVIVQQLIGSQKGMYFYPEVSGVGFSRNYRRWTDRIKIEDGVVRLVFGLGTKCTGR
GYARIFSLTNLRLRPEGNNPKEIAKNSQETFDVLNLITGEVETYNINEVPQFLTYHDKIGDIAQIYSKRENAIFDINILN
NSNDVFNKYIFTFENFPRKHRGFFNIISNLFKYLEEEMGMAVDIEFTYDLKKGEFYLLQTRPLSSYESFRKVHIPKDIEK
KCVLLKGDRMLTNGILKNIKYIVYVDHKVYSQYRDKHEIARIIGRLNKSIGDRYILVGPGRWGSSNPYLGVPVIYNEISN
AAMIVELGIKNGDFMPELSYGTHFFTDLDIDNILYMPVFDGYENNIFNEEWFKRGTYIDTAVKIFEGRFDAYLDGDKMIG
YVISHEK

Sequences:

>Translated_567_residues
MSETLLYKNYNPRLNRKNEDRIFGEGYIGGKASGLCFAEEVLEKYIDVFKQSVKIPESFFIATDYYQMFLDYNNLNDITE
DTPYEEVEMRFREAKFPPEFKKVLTEILNKMYYPLAVRSSSLLEDNVKYSFAGKYYTTFIPNKGTDRERLKQLEKAIKEI
YVSVYGPDAVAYRRKHAPDQKELMGVIVQQLIGSQKGMYFYPEVSGVGFSRNYRRWTDRIKIEDGVVRLVFGLGTKCTGR
GYARIFSLTNLRLRPEGNNPKEIAKNSQETFDVLNLITGEVETYNINEVPQFLTYHDKIGDIAQIYSKRENAIFDINILN
NSNDVFNKYIFTFENFPRKHRGFFNIISNLFKYLEEEMGMAVDIEFTYDLKKGEFYLLQTRPLSSYESFRKVHIPKDIEK
KCVLLKGDRMLTNGILKNIKYIVYVDHKVYSQYRDKHEIARIIGRLNKSIGDRYILVGPGRWGSSNPYLGVPVIYNEISN
AAMIVELGIKNGDFMPELSYGTHFFTDLDIDNILYMPVFDGYENNIFNEEWFKRGTYIDTAVKIFEGRFDAYLDGDKMIG
YVISHEK
>Mature_566_residues
SETLLYKNYNPRLNRKNEDRIFGEGYIGGKASGLCFAEEVLEKYIDVFKQSVKIPESFFIATDYYQMFLDYNNLNDITED
TPYEEVEMRFREAKFPPEFKKVLTEILNKMYYPLAVRSSSLLEDNVKYSFAGKYYTTFIPNKGTDRERLKQLEKAIKEIY
VSVYGPDAVAYRRKHAPDQKELMGVIVQQLIGSQKGMYFYPEVSGVGFSRNYRRWTDRIKIEDGVVRLVFGLGTKCTGRG
YARIFSLTNLRLRPEGNNPKEIAKNSQETFDVLNLITGEVETYNINEVPQFLTYHDKIGDIAQIYSKRENAIFDINILNN
SNDVFNKYIFTFENFPRKHRGFFNIISNLFKYLEEEMGMAVDIEFTYDLKKGEFYLLQTRPLSSYESFRKVHIPKDIEKK
CVLLKGDRMLTNGILKNIKYIVYVDHKVYSQYRDKHEIARIIGRLNKSIGDRYILVGPGRWGSSNPYLGVPVIYNEISNA
AMIVELGIKNGDFMPELSYGTHFFTDLDIDNILYMPVFDGYENNIFNEEWFKRGTYIDTAVKIFEGRFDAYLDGDKMIGY
VISHEK

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 66227; Mature: 66096

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLLYKNYNPRLNRKNEDRIFGEGYIGGKASGLCFAEEVLEKYIDVFKQSVKIPESFF
CCCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEE
IATDYYQMFLDYNNLNDITEDTPYEEVEMRFREAKFPPEFKKVLTEILNKMYYPLAVRSS
EEHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCC
SLLEDNVKYSFAGKYYTTFIPNKGTDRERLKQLEKAIKEIYVSVYGPDAVAYRRKHAPDQ
HHHHCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCH
KELMGVIVQQLIGSQKGMYFYPEVSGVGFSRNYRRWTDRIKIEDGVVRLVFGLGTKCTGR
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHCEEEECCCCEEEEECCCCCCCCC
GYARIFSLTNLRLRPEGNNPKEIAKNSQETFDVLNLITGEVETYNINEVPQFLTYHDKIG
CCEEEEEEECEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
DIAQIYSKRENAIFDINILNNSNDVFNKYIFTFENFPRKHRGFFNIISNLFKYLEEEMGM
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
AVDIEFTYDLKKGEFYLLQTRPLSSYESFRKVHIPKDIEKKCVLLKGDRMLTNGILKNIK
EEEEEEEEEECCCCEEEEECCCCHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHHCCE
YIVYVDHKVYSQYRDKHEIARIIGRLNKSIGDRYILVGPGRWGSSNPYLGVPVIYNEISN
EEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECHHHHHCCCC
AAMIVELGIKNGDFMPELSYGTHFFTDLDIDNILYMPVFDGYENNIFNEEWFKRGTYIDT
CEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHH
AVKIFEGRFDAYLDGDKMIGYVISHEK
HHHHHHCCCCEEECCCCEEEEEEECCC
>Mature Secondary Structure 
SETLLYKNYNPRLNRKNEDRIFGEGYIGGKASGLCFAEEVLEKYIDVFKQSVKIPESFF
CCCEEEECCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEE
IATDYYQMFLDYNNLNDITEDTPYEEVEMRFREAKFPPEFKKVLTEILNKMYYPLAVRSS
EEHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEECCC
SLLEDNVKYSFAGKYYTTFIPNKGTDRERLKQLEKAIKEIYVSVYGPDAVAYRRKHAPDQ
HHHHCCCCEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCH
KELMGVIVQQLIGSQKGMYFYPEVSGVGFSRNYRRWTDRIKIEDGVVRLVFGLGTKCTGR
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHCEEEECCCCEEEEECCCCCCCCC
GYARIFSLTNLRLRPEGNNPKEIAKNSQETFDVLNLITGEVETYNINEVPQFLTYHDKIG
CCEEEEEEECEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHH
DIAQIYSKRENAIFDINILNNSNDVFNKYIFTFENFPRKHRGFFNIISNLFKYLEEEMGM
HHHHHHHCCCCEEEEEEEECCCCHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
AVDIEFTYDLKKGEFYLLQTRPLSSYESFRKVHIPKDIEKKCVLLKGDRMLTNGILKNIK
EEEEEEEEEECCCCEEEEECCCCHHHHHHHEECCCCCCCCCEEEEECCHHHHHHHHHCCE
YIVYVDHKVYSQYRDKHEIARIIGRLNKSIGDRYILVGPGRWGSSNPYLGVPVIYNEISN
EEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEECHHHHHCCCC
AAMIVELGIKNGDFMPELSYGTHFFTDLDIDNILYMPVFDGYENNIFNEEWFKRGTYIDT
CEEEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHH
AVKIFEGRFDAYLDGDKMIGYVISHEK
HHHHHHCCCCEEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA