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The map label for this gene is tnpR [H]

Identifier: 162149379

GI number: 162149379

Start: 3694948

End: 3695514

Strand: Direct

Name: tnpR [H]

Synonym: GDI_3613

Alternate gene names: 162149379

Gene position: 3694948-3695514 (Clockwise)

Preceding gene: 162149376

Following gene: 162149380

Centisome position: 93.68

GC content: 67.2

Gene sequence:

>567_bases
ATGCGGATCGGATACGCGCGAACCAGCACGACGGATCAGGAGGCAGGGCTGGCCGCCCAGCGGAGGGACCTTGAAGCATT
CGGTTGCGACTGGCTGTTCTCGGAGCAGGTGTCGGGCACCCTCACCGTCAGGCCGGAACTGGAAGCGGCCATCGACTATC
TGCGCCGGGGTGACGCGCTGGTGGTGTGCAAGCCGGACCGCCTGGCGCGATCAACGGCCGACCTGCTGGCGTTCGTGGAG
CGCATCAAGGCCAAGGGGTGCGAACTGGTCATTCTGTCGATGGGCGGCCAGACGCTGGATACCAGCAGCCCGACCAGCAA
GCTGATGCTGTCGATGCTCGCGGCGGTCGCCGAGTTCGAGCGTGACCTGATGCTGGAGCGGCAACGGGAAGGGATCGCCA
AGGCCAAGGCGGAAGGGCGCTATCTGGGGCGGAAACCCACCGCGCGGGCACAGGCCGAAGAGGTCCAGAAGCTGGCAGCG
GAGGGGGTCAGCCCCACCGAGATCGCCAAGCGGCTGGGGATCGGGCGGACGTCCGTCTATCGGTGCCTGGGAAAGGTCGC
AGCCTGA

Upstream 100 bases:

>100_bases
CAATAGGGCTTGCCCCAGCGGAACATTCGAAGCGGAACAGATTTGTGTTCCAATAAAATACCGTTTGATGTTTTGGAACA
GATCGCCGAAGGAACAACCC

Downstream 100 bases:

>100_bases
CGATTGTCCGCAGGCGTCCGTGGCCGTCCCTGGCAAGCCCCGTTTCTAAGAGGGGTAGCAAGAGGGGTAAATTTCCGACC
TCTAGCCTAATATTCTGATT

Product: resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MRIGYARTSTTDQEAGLAAQRRDLEAFGCDWLFSEQVSGTLTVRPELEAAIDYLRRGDALVVCKPDRLARSTADLLAFVE
RIKAKGCELVILSMGGQTLDTSSPTSKLMLSMLAAVAEFERDLMLERQREGIAKAKAEGRYLGRKPTARAQAEEVQKLAA
EGVSPTEIAKRLGIGRTSVYRCLGKVAA

Sequences:

>Translated_188_residues
MRIGYARTSTTDQEAGLAAQRRDLEAFGCDWLFSEQVSGTLTVRPELEAAIDYLRRGDALVVCKPDRLARSTADLLAFVE
RIKAKGCELVILSMGGQTLDTSSPTSKLMLSMLAAVAEFERDLMLERQREGIAKAKAEGRYLGRKPTARAQAEEVQKLAA
EGVSPTEIAKRLGIGRTSVYRCLGKVAA
>Mature_188_residues
MRIGYARTSTTDQEAGLAAQRRDLEAFGCDWLFSEQVSGTLTVRPELEAAIDYLRRGDALVVCKPDRLARSTADLLAFVE
RIKAKGCELVILSMGGQTLDTSSPTSKLMLSMLAAVAEFERDLMLERQREGIAKAKAEGRYLGRKPTARAQAEEVQKLAA
EGVSPTEIAKRLGIGRTSVYRCLGKVAA

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=182, Percent_Identity=36.2637362637363, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787827, Length=189, Percent_Identity=35.4497354497354, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1787638, Length=189, Percent_Identity=35.4497354497354, Blast_Score=103, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20495; Mature: 20495

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGYARTSTTDQEAGLAAQRRDLEAFGCDWLFSEQVSGTLTVRPELEAAIDYLRRGDAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCHHHHHHHHHCCCEE
VVCKPDRLARSTADLLAFVERIKAKGCELVILSMGGQTLDTSSPTSKLMLSMLAAVAEFE
EEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
RDLMLERQREGIAKAKAEGRYLGRKPTARAQAEEVQKLAAEGVSPTEIAKRLGIGRTSVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHH
RCLGKVAA
HHHHHHCC
>Mature Secondary Structure
MRIGYARTSTTDQEAGLAAQRRDLEAFGCDWLFSEQVSGTLTVRPELEAAIDYLRRGDAL
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCHHHHHHHHHCCCEE
VVCKPDRLARSTADLLAFVERIKAKGCELVILSMGGQTLDTSSPTSKLMLSMLAAVAEFE
EEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
RDLMLERQREGIAKAKAEGRYLGRKPTARAQAEEVQKLAAEGVSPTEIAKRLGIGRTSVY
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHH
RCLGKVAA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]