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The map label for this gene is rlmB [H]

Identifier: 162149380

GI number: 162149380

Start: 3696024

End: 3696725

Strand: Direct

Name: rlmB [H]

Synonym: GDI_3614

Alternate gene names: 162149380

Gene position: 3696024-3696725 (Clockwise)

Preceding gene: 162149379

Following gene: 162149381

Centisome position: 93.71

GC content: 71.65

Gene sequence:

>702_bases
CTGTACGGGCTGCATCCCGCGCTGGCGGCGCTGGGCAACCCGGAACGCCGCATCCGCCAGATCCTGGCGACCGAGGACGG
CGAGGCCGCGCTGCGCGAGCGCCTGAATGCTCCCCTGCCGATGCAGCCCCACCGCACCGATCGCGCCCGGCTGGAATCGC
TGTGCGGGCGCGACGCCGTTCACCAGGGAATCGCGGTCCTGGCCGATGCGCTGCCGTCGCTGGCGATCGAGGACGTGACC
GGGCGGCCCGGCCCCCTTCTGCTGCTGGACCAGGTGACCGATCCGCGCAATGTCGGCGCCATCCTGCGCTCCGCCGCCGC
CTTCGGCGCCGCCGCCGTGGTGGTGATGGAACGCAACGCGCCGGACGAGACCGGTGCCCTGGCCAAGGCCGCGTCGGGGG
CGCTGGAAATCGTGCCGCTGGGTGCGGTGGTGAACCTGGCCCGCACGCTGGACGCGCTGAAGAGCCTGGATTTCTGGGTC
GTGGGGCTGGATGCCGGCGGCGGAATACTGGACGGCACCAGTTTCGGCCAGCGGCGTGTGGCGCTGGTCCTGGGGGCCGA
GGGCGACGGGTTGCGCCGCCTGACCCGCGAGCATTGCGACGAAATCGCCGGACTGGCGATGCCCGGCGACATGGAAAGCC
TGAACGTCTCCAACGCCGCCGCCGTCGCCCTGTACGAACTGATACGGCGCGGAAAGGCATAA

Upstream 100 bases:

>100_bases
GCCCCCGGCCTCGGGTACATCCCCGGGCGCGGCCCCGGGCCGCCAGGGGCGGCGCGGCGGCGGCGCGGCACGCGGCGGCC
CCCCGCGTGGCACCTACTGG

Downstream 100 bases:

>100_bases
TCTTAAAATCGCGCTTACCCCGCTGACCTCGCCGCCGGTCCGTGGCACGGTCTGCCGATGCAGCAGAGATGGAGATCCGG
AATGAAGCCTGCCCTGCCCA

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase rlmB

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MYGLHPALAALGNPERRIRQILATEDGEAALRERLNAPLPMQPHRTDRARLESLCGRDAVHQGIAVLADALPSLAIEDVT
GRPGPLLLLDQVTDPRNVGAILRSAAAFGAAAVVVMERNAPDETGALAKAASGALEIVPLGAVVNLARTLDALKSLDFWV
VGLDAGGGILDGTSFGQRRVALVLGAEGDGLRRLTREHCDEIAGLAMPGDMESLNVSNAAAVALYELIRRGKA

Sequences:

>Translated_233_residues
MYGLHPALAALGNPERRIRQILATEDGEAALRERLNAPLPMQPHRTDRARLESLCGRDAVHQGIAVLADALPSLAIEDVT
GRPGPLLLLDQVTDPRNVGAILRSAAAFGAAAVVVMERNAPDETGALAKAASGALEIVPLGAVVNLARTLDALKSLDFWV
VGLDAGGGILDGTSFGQRRVALVLGAEGDGLRRLTREHCDEIAGLAMPGDMESLNVSNAAAVALYELIRRGKA
>Mature_233_residues
MYGLHPALAALGNPERRIRQILATEDGEAALRERLNAPLPMQPHRTDRARLESLCGRDAVHQGIAVLADALPSLAIEDVT
GRPGPLLLLDQVTDPRNVGAILRSAAAFGAAAVVVMERNAPDETGALAKAASGALEIVPLGAVVNLARTLDALKSLDFWV
VGLDAGGGILDGTSFGQRRVALVLGAEGDGLRRLTREHCDEIAGLAMPGDMESLNVSNAAAVALYELIRRGKA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=260, Percent_Identity=29.6153846153846, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790623, Length=248, Percent_Identity=37.5, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1788935, Length=194, Percent_Identity=30.9278350515464, Blast_Score=60, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17555908, Length=249, Percent_Identity=28.5140562248996, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 24384; Mature: 24384

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYGLHPALAALGNPERRIRQILATEDGEAALRERLNAPLPMQPHRTDRARLESLCGRDAV
CCCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHH
HQGIAVLADALPSLAIEDVTGRPGPLLLLDQVTDPRNVGAILRSAAAFGAAAVVVMERNA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCC
PDETGALAKAASGALEIVPLGAVVNLARTLDALKSLDFWVVGLDAGGGILDGTSFGQRRV
CCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEE
ALVLGAEGDGLRRLTREHCDEIAGLAMPGDMESLNVSNAAAVALYELIRRGKA
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MYGLHPALAALGNPERRIRQILATEDGEAALRERLNAPLPMQPHRTDRARLESLCGRDAV
CCCCCHHHHHCCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHHH
HQGIAVLADALPSLAIEDVTGRPGPLLLLDQVTDPRNVGAILRSAAAFGAAAVVVMERNA
HHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCC
PDETGALAKAASGALEIVPLGAVVNLARTLDALKSLDFWVVGLDAGGGILDGTSFGQRRV
CCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEE
ALVLGAEGDGLRRLTREHCDEIAGLAMPGDMESLNVSNAAAVALYELIRRGKA
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]